Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-GAS6 Polyclonal Antibody, Unconjugated(bs-7549R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
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- bs-7549R
- 2025年10月24日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-7549R |
| 英文名称 | GAS 6 Rabbit pAb |
| 中文名称 | 生长停滞特异性蛋白6抗体 |
| 英文别名 | AXL receptor tyrosine kinase ligand; AXL stimulatory factor; AXLLG; AXS F; AXSF; Gas 6; GAS6; Growth-arrest-specific protein 6; Growth-arrest-specific protein 6 precursor. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat, Chicken, Dog, Pig, Cow, Horse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human GAS 6 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 76 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cardiovascular > Atherosclerosis > Thrombosis Signal Transduction > Protein Phosphorylation > Tyrosine Kinases > Receptor Tyrosine Kinases Stem Cells > Hematopoietic Progenitors > Myeloid > Thrombocytic Lineage Stem Cells > Mesenchymal Stem Cells > Chondrogenesis |
| 亚基 | Heterodimer and heterotetramer with AXL. |
| 亚细胞定位 | Secreted. |
| 组织特异性 | Plasma. Isoform 1 and isoform 2 are widely expressed. Isoform 1 is the predominant form in spleen. |
| 翻译后修饰 | Isoform 1 is proteolytically processed after secretion to yield a N-terminal 36 kDa protein and a C-terminal 50 kDa protein including the laminin G-like domains which activates AXL. |
| 相似性 | Contains 4 EGF-like domains. Contains 1 Gla (gamma-carboxy-glutamate) domain. Contains 2 laminin G-like domains. |
| 功能 | Ligand for tyrosine-protein kinase receptors AXL, TYRO3 and MER whose signaling is implicated in cell growth and survival, cell adhesion and cell migration. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | GAS 6 is a ligand for the tyrosine-protein kinase receptors AXL, TYRO3 and MER whose signaling is implicated in cell growth and survival, cell adhesion and cell migration. GAS 6 regulates chondrogenic differentiation from mesenchymal cells and also plays a role in thrombosis by amplifying platelet aggregation and secretion in response to known agonists. It is over expressed in human gliomas and regulate the phagocytic function of sertoli cells. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Sample:
Liver (Mouse) Lysate at 40 ug
Kidney (Mouse) Lysate at 40 ug
Primary: Anti-GAS 6 (bs-7549R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 76 kD
Observed band size: 76 kD
Liver (Mouse) Lysate at 40 ug
Kidney (Mouse) Lysate at 40 ug
Primary: Anti-GAS 6 (bs-7549R) at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 76 kD
Observed band size: 76 kD
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文献和实验该产品被引用文献
[IF={{ 8.322 }}] {Zhou, Hang. et al. AXL kinase-mediated astrocytic phagocytosis modulates outcomes of traumatic brain injury. J Neuroinflamm. 2021 Dec;18(1):1-17} {WB,IF} {Mouse}
[IF={{ 6.684 }}] {Shanyu Fang. et al. NTNG1 Modulates Cisplatin Resistance in Epithelial Ovarian Cancer Cells via the GAS6/AXL/Akt Pathway. Front Cell Dev Biol. 2021; 9: 652325} {WB} {Human}
[IF={{ 6.3 }}] {Aizhen Zhao. et al. Mangiferin Attenuates Myocardial Ischemia Reperfusion Injury by Regulating the GAS6/Axl Signaling Pathway. PHYTOTHER RES. 2025 Jan;:} {WB} {Mouse}
[IF={{ 5.882 }}] {Wangrui Lei. et al. Psoralidin protects against cerebral hypoxia/reoxygenation injury: Role of GAS6/Axl signaling. PHYTOTHER RES. 2022 May 18} {WB} {Human,Mouse}
[IF={{ 5.6 }}] {Xiaoyue Guan. et al. The Role of Macrophage Efferocytosis in the Pathogenesis of Apical Periodontitis. INT J MOL SCI. 2024 Jan;25(7):3854} {IHC,IF} {Human}
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GAS 6 Rabbit pAb(bs-7549R)-50ul/100ul/200ul
￥1180 - 2800
