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FCAR/CD89 Rabbit pAb(bs-6586R)

-50ul/100ul/200ul
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  • ￥1180 - 2800
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  • bs-6586R
  • 2025年10月24日
  • 产品信息以Bioss网站为准
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      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-6586R
    英文名称FCAR/CD89 Rabbit pAb
    中文名称免疫球蛋白A Fc段受体1抗体
    英文别名CD89; Fc alpha receptor; FCAR; FCAR_HUMAN; IgA Fc receptor; Immunoglobulin alpha Fc receptor.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Rat
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human FCAR
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量29 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    亚基Associates with the Fc epsilon RI gamma 2 receptor inducing tyrosine phosphorylation of gamma 2.
    亚细胞定位Isoform A.1: Cell membrane; Single-pass type I membrane protein.
    Isoform A.2: Cell membrane; Single-pass type I membrane protein.
    Isoform A.3: Cell membrane; Single-pass type I membrane protein.
    Isoform B: Secreted.
    Isoform B-delta-S2: Secreted.
    组织特异性Isoform A.1, isoform A.2 and isoform A.3 are differentially expressed between blood and mucosal myeloid cells. Isoform A.1, isoform A.2 and isoform A.3 are expressed in monocytes. Isoform A.1 and isoform A.2 are expressed in alveolar macrophages; however only one isoform is expressed at alveolar macrophages surfaces.
    相似性Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料Binds to the Fc region of immunoglobulins alpha. Mediates several functions including cytokine production. Tissue specificity;Isoform A.1, isoform A.2 and isoform A.3 are differentially expressed between blood and mucosal myeloid cells. Isoform A.1, isoform A.2 and isoform A.3 are expressed in monocytes. Isoform A.1 and isoform A.2 are expressed in alveolar macrophages; however only one isoform is expressed at alveolar macrophages surfaces.

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}
    {Flow-Cyt}{2ug/Test}

     

    产品细节图片1
    P‌‌araformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FCAR) Polyclonal Antibody, Unconjugated (bs-6586R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
    产品细节图片2
    P‌‌araformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FCAR) Polyclonal Antibody, Unconjugated (bs-6586R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
    产品细节图片3
    P‌‌araformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FCAR) Polyclonal Antibody, Unconjugated (bs-6586R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
    产品细节图片4
    P‌‌araformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FCAR) Polyclonal Antibody, Unconjugated (bs-6586R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
    产品细节图片5
    P‌‌araformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FCAR) Polyclonal Antibody, Unconjugated (bs-6586R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
    产品细节图片6
    Sample: Lane 1: Rat Liver tissue lysates Lane 2: Rat H9C2 cell lysates Lane 3: Human LOVO cell lysates Lane 4: Human K562 cell lysates Lane 5: Human HL-60 cell lysates Primary: Anti-FCAR/CD89 (bs-6586R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 29 kDa Observed band size: 50 kDa
    产品细节图片7
    Blank control:Jurkat.
    Primary Antibody (green line): Rabbit Anti-CD89 antibody (bs-6586R)
    Dilution: 2ug/Test;
    Secondary Antibody (white blue line) : Goat anti-rabbit IgG-FITC
    Dilution: 0.5ug/Test.
    Isotype control（orange line）：Normal Rabbit IgG
    Protocol
    The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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