FCAR | CD89/FcaRI Rabbit pAb(b

P‌‌araformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FCAR) Polyclonal Antibody, Unconjugated (bs-6586R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

P‌‌araformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FCAR) Polyclonal Antibody, Unconjugated (bs-6586R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

P‌‌araformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FCAR) Polyclonal Antibody, Unconjugated (bs-6586R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. _x000D_ _x000D_

P‌‌araformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FCAR) Polyclonal Antibody, Unconjugated (bs-6586R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

P‌‌araformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FCAR) Polyclonal Antibody, Unconjugated (bs-6586R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

Sample:_x000D_ Lane 1: Rat Liver tissue lysates_x000D_ Lane 2: Rat H9C2 cell lysates_x000D_ Lane 3: Human LOVO cell lysates_x000D_ Lane 4: Human K562 cell lysates_x000D_ Lane 5: Human HL-60 cell lysates_x000D_ Primary: Anti-FCAR/CD89 (bs-6586R) at 1/1000 dilution_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution_x000D_ Predicted band size: 29 kDa_x000D_ Observed band size: 50 kDa

Blank control:Jurkat.
_x000D_ Primary Antibody (green line): Rabbit Anti-CD89 antibody (bs-6586R)
_x000D_ Dilution: 2ug/Test;
_x000D_ Secondary Antibody (white blue line) : Goat anti-rabbit IgG-FITC
_x000D_ Dilution: 0.5ug/Test.
_x000D_ Isotype control（orange line）：Normal Rabbit IgG
_x000D_ Protocol
_x000D_ The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.