pFD666

名称	编号	规格	价格	手册
pFD666

质粒编号:
质粒名称: pFD666
其他名称:
质粒大小（bp）: 5250
抗性:
质粒类型: Unspecified
是否病毒:
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组成型或诱导性:
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GenBank号:
其他属性: The order of the major features in this plasmid is:rrnC terminator – SP6 – SphI/MCS/HindIII – T7 – trpA terminator – neo – synthetic promoter -pMB1 ori – cos – M13 ori – pJV1 ori. [1] Cosmid shuttle vector permitting production of ssDNA and containing promoters for in vitro RNA synthesis. Terminators flanking the polylinker protect the vector from transcripts originating in cloned inserts. [1] The ori of pJV1 from Streptomyces phaeochromogenes is compatible with plasmids derived from pIJ101. This vector can be used for complementation studies using multiple cloned genes in one host. [1] M13K07 helper phage improves the yield of ssDNA in E.coli with this vector. M13mp18 may also be used. [1] The neo promoter was replaced by a synthetic promoter recognized by both hosts. [1] Restriction digests of the clone give the following sizes (kb): BamHI–5.4; PstI–5.4; PvuII–3.7, 1.7; SacI–4.5, 0.9. (ATCC staff) Medium is 1236 LB plus kanamycin. Hosts: E.coli, Actinomycetes, Streptomyces lividans, Saccharopolyspora erythraea. Related vectors: pFDNEO, pJV1.
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