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文献和实验Reduced Glutathione and Glutathione Disulfide
Glutathione (GSH; L- -glutamyl-L-cysteinl-glycine) plays an important role in the prevention of radical mediated injury to the body. It does so as a radical scavenger and by supplying GSH to the antioxidant enzymes described in Chapter 29
Measurement of Oxidized/Reduced Glutathione Ratio
, acting as a free radical scavenger and inhibitor of lipid peroxidation. GSH also participates in the detoxification of hydrogen peroxide by various glutathione peroxidases. The ratio of reduced GSH to oxidized GSH (GSSG) is an indicator of cellular health
Measurement of Reduced Glutathione Using High-Pressure Liquid Chromatography
As outlined in Chapter 8 , glutathione in the intact cell is maintained predominantly in its reduced form by the cytosolic enzyme, glutathione reductase. Cell lysis can lead to rapid oxidation to the oxidized form, GSSG, and degradation by γ
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