pET-30c(+)

名称	编号	规格	价格	手册
pET-30c(+)(pET30c)(60908-2832)	60908-2832

质粒编号: 60908-2832
质粒名称: pET-30c(+)
其他名称: pET30c, pet 30c
质粒大小（bp）: 5423
抗性: Kanamycin (卡那霉素)
质粒类型: 大肠杆菌表达载体
是否病毒: Nonviral
启动子:
稳转或瞬转: Transient
组成型或诱导性: Constitutive
表达水平: High
蛋白标签: N-His, N-S, N-Thrombin, N-EK, C-His
5′ 测序引物:T7

5′ 测序引物序列: 5′-TAATACGACTCACTATAGGG-3′
GenBank号:
其他属性: Nterm thrombin cleavage site; Nterm enterokinase cleavage site; a,b,c vary by MCS

质粒图谱:

Feature Name	Start	End
T7_terminator	129	1
T7_Terminal_primer	69	87
EK	231	220
lacI	303	1909
T7_transl_en_RBS	373	357
lacO	418	391
T7_promoter	436	418
tet (300 – 563)	472	735
pBRrevBam_primer	543	524
tet (576 – 851)	1968	2243
ROP	2718	2909
pGEX_3_primer	2925	2903
pBR322_origin	3943	3324
KanR2	4049	4864

ORF	Start	End
ORF frame 1	328	1077
ORF frame 2	950	1909
ORF frame 2	4049	4864

Enzyme Name	Cut
XhoI	158
NotI	166
EagI	166
HindIII	173
SalI	179
SacI	190
EcoRI	192
BamHI	198
EcoRV	209
NcoI	213
KpnI	239
BglII	242
BstBI	269
NdeI	347
XbaI	385
BclI	1191
ApaI	1388
HpaI	1683
FspI	2259
NruI	4137
SmaI	4354
XmaI	4352

质粒序列:

1	atccggatat	agttcctcct	ttcagcaaaa	aacccctcaa	gacccgttta	50
51	gaggccccaa	ggggttatgc	tagttattgc	tcagcggtgg	cagcagccaa	100
101	ctcagcttcc	tttcgggctt	tgttagcagc	cggatctcag	tggtggtggt	150
151	ggtggtgctc	gagtgcggcc	gcaagcttgt	cgacggagct	cgaattcgga	200
201	tccacagata	tcccatggcc	ttgtcgtcgt	cgtcggtacc	cagatctggg	250
251	ctgtccatgt	gctggcgttc	gaatttagca	gcagcggttt	ctttcatacc	300
301	agaaccgcgt	ggcaccagac	cagaagaatg	atgatgatga	tggtgcatat	350
351	gtatatctcc	ttcttaaagt	taaacaaaat	tatttctaga	ggggaattgt	400
401	tatccgctca	caattcccct	atagtgagtc	gtattaattt	cgcgggatcg	450
451	agatcgatct	cgatcctcta	cgccggacgc	atcgtggccg	gcatcaccgg	500
501	cgccacaggt	gcggttgctg	gcgcctatat	cgccgacatc	accgatgggg	550
551	aagatcgggc	tcgccacttc	gggctcatga	gcgcttgttt	cggcgtgggt	600
601	atggtggcag	gccccgtggc	cgggggactg	ttgggcgcca	tctccttgca	650
651	tgcaccattc	cttgcggcgg	cggtgctcaa	cggcctcaac	ctactactgg	700
701	gctgcttcct	aatgcaggag	tcgcataagg	gagagcgtcg	agatcccgga	750
751	caccatcgaa	tggcgcaaaa	cctttcgcgg	tatggcatga	tagcgcccgg	800
801	aagagagtca	attcagggtg	gtgaatgtga	aaccagtaac	gttatacgat	850
851	gtcgcagagt	atgccggtgt	ctcttatcag	accgtttccc	gcgtggtgaa	900
901	ccaggccagc	cacgtttctg	cgaaaacgcg	ggaaaaagtg	gaagcggcga	950
951	tggcggagct	gaattacatt	cccaaccgcg	tggcacaaca	actggcgggc	1000
1001	aaacagtcgt	tgctgattgg	cgttgccacc	tccagtctgg	ccctgcacgc	1050
1051	gccgtcgcaa	attgtcgcgg	cgattaaatc	tcgcgccgat	caactgggtg	1100
1101	ccagcgtggt	ggtgtcgatg	gtagaacgaa	gcggcgtcga	agcctgtaaa	1150
1151	gcggcggtgc	acaatcttct	cgcgcaacgc	gtcagtgggc	tgatcattaa	1200
1201	ctatccgctg	gatgaccagg	atgccattgc	tgtggaagct	gcctgcacta	1250
1251	atgttccggc	gttatttctt	gatgtctctg	accagacacc	catcaacagt	1300
1301	attattttct	cccatgaaga	cggtacgcga	ctgggcgtgg	agcatctggt	1350
1351	cgcattgggt	caccagcaaa	tcgcgctgtt	agcgggccca	ttaagttctg	1400
1401	tctcggcgcg	tctgcgtctg	gctggctggc	ataaatatct	cactcgcaat	1450
1451	caaattcagc	cgatagcgga	acgggaaggc	gactggagtg	ccatgtccgg	1500
1501	ttttcaacaa	accatgcaaa	tgctgaatga	gggcatcgtt	cccactgcga	1550
1551	tgctggttgc	caacgatcag	atggcgctgg	gcgcaatgcg	cgccattacc	1600
1601	gagtccgggc	tgcgcgttgg	tgcggacatc	tcggtagtgg	gatacgacga	1650
1651	taccgaagac	agctcatgtt	atatcccgcc	gttaaccacc	atcaaacagg	1700
1701	attttcgcct	gctggggcaa	accagcgtgg	accgcttgct	gcaactctct	1750
1751	cagggccagg	cggtgaaggg	caatcagctg	ttgcccgtct	cactggtgaa	1800
1801	aagaaaaacc	accctggcgc	ccaatacgca	aaccgcctct	ccccgcgcgt	1850
1851	tggccgattc	attaatgcag	ctggcacgac	aggtttcccg	actggaaagc	1900
1901	gggcagtgag	cgcaacgcaa	ttaatgtaag	ttagctcact	cattaggcac	1950
1951	cgggatctcg	accgatgccc	ttgagagcct	tcaacccagt	cagctccttc	2000
2001	cggtgggcgc	ggggcatgac	tatcgtcgcc	gcacttatga	ctgtcttctt	2050
2051	tatcatgcaa	ctcgtaggac	aggtgccggc	agcgctctgg	gtcattttcg	2100
2101	gcgaggaccg	ctttcgctgg	agcgcgacga	tgatcggcct	gtcgcttgcg	2150
2151	gtattcggaa	tcttgcacgc	cctcgctcaa	gccttcgtca	ctggtcccgc	2200
2201	caccaaacgt	ttcggcgaga	agcaggccat	tatcgccggc	atggcggccc	2250
2251	cacgggtgcg	catgatcgtg	ctcctgtcgt	tgaggacccg	gctaggctgg	2300
2301	cggggttgcc	ttactggtta	gcagaatgaa	tcaccgatac	gcgagcgaac	2350
2351	gtgaagcgac	tgctgctgca	aaacgtctgc	gacctgagca	acaacatgaa	2400
2401	tggtcttcgg	tttccgtgtt	tcgtaaagtc	tggaaacgcg	gaagtcagcg	2450
2451	ccctgcacca	ttatgttccg	gatctgcatc	gcaggatgct	gctggctacc	2500
2501	ctgtggaaca	cctacatctg	tattaacgaa	gcgctggcat	tgaccctgag	2550
2551	tgatttttct	ctggtcccgc	cgcatccata	ccgccagttg	tttaccctca	2600
2601	caacgttcca	gtaaccgggc	atgttcatca	tcagtaaccc	gtatcgtgag	2650
2651	catcctctct	cgtttcatcg	gtatcattac	ccccatgaac	agaaatcccc	2700
2701	cttacacgga	ggcatcagtg	accaaacagg	aaaaaaccgc	ccttaacatg	2750
2751	gcccgcttta	tcagaagcca	gacattaacg	cttctggaga	aactcaacga	2800
2801	gctggacgcg	gatgaacagg	cagacatctg	tgaatcgctt	cacgaccacg	2850
2851	ctgatgagct	ttaccgcagc	tgcctcgcgc	gtttcggtga	tgacggtgaa	2900
2901	aacctctgac	acatgcagct	cccggagacg	gtcacagctt	gtctgtaagc	2950
2951	ggatgccggg	agcagacaag	cccgtcaggg	cgcgtcagcg	ggtgttggcg	3000
3001	ggtgtcgggg	cgcagccatg	acccagtcac	gtagcgatag	cggagtgtat	3050
3051	actggcttaa	ctatgcggca	tcagagcaga	ttgtactgag	agtgcaccat	3100
3101	atatgcggtg	tgaaataccg	cacagatgcg	taaggagaaa	ataccgcatc	3150
3151	aggcgctctt	ccgcttcctc	gctcactgac	tcgctgcgct	cggtcgttcg	3200
3201	gctgcggcga	gcggtatcag	ctcactcaaa	ggcggtaata	cggttatcca	3250
3251	cagaatcagg	ggataacgca	ggaaagaaca	tgtgagcaaa	aggccagcaa	3300
3301	aaggccagga	accgtaaaaa	ggccgcgttg	ctggcgtttt	tccataggct	3350
3351	ccgcccccct	gacgagcatc	acaaaaatcg	acgctcaagt	cagaggtggc	3400
3401	gaaacccgac	aggactataa	agataccagg	cgtttccccc	tggaagctcc	3450
3451	ctcgtgcgct	ctcctgttcc	gaccctgccg	cttaccggat	acctgtccgc	3500
3501	ctttctccct	tcgggaagcg	tggcgctttc	tcatagctca	cgctgtaggt	3550
3551	atctcagttc	ggtgtaggtc	gttcgctcca	agctgggctg	tgtgcacgaa	3600
3601	ccccccgttc	agcccgaccg	ctgcgcctta	tccggtaact	atcgtcttga	3650
3651	gtccaacccg	gtaagacacg	acttatcgcc	actggcagca	gccactggta	3700
3701	acaggattag	cagagcgagg	tatgtaggcg	gtgctacaga	gttcttgaag	3750
3751	tggtggccta	actacggcta	cactagaagg	acagtatttg	gtatctgcgc	3800
3801	tctgctgaag	ccagttacct	tcggaaaaag	agttggtagc	tcttgatccg	3850
3851	gcaaacaaac	caccgctggt	agcggtggtt	tttttgtttg	caagcagcag	3900
3901	attacgcgca	gaaaaaaagg	atctcaagaa	gatcctttga	tcttttctac	3950
3951	ggggtctgac	gctcagtgga	acgaaaactc	acgttaaggg	attttggtca	4000
4001	tgaacaataa	aactgtctgc	ttacataaac	agtaatacaa	ggggtgttat	4050
4051	gagccatatt	caacgggaaa	cgtcttgctc	taggccgcga	ttaaattcca	4100
4101	acatggatgc	tgatttatat	gggtataaat	gggctcgcga	taatgtcggg	4150
4151	caatcaggtg	cgacaatcta	tcgattgtat	gggaagcccg	atgcgccaga	4200
4201	gttgtttctg	aaacatggca	aaggtagcgt	tgccaatgat	gttacagatg	4250
4251	agatggtcag	actaaactgg	ctgacggaat	ttatgcctct	tccgaccatc	4300
4301	aagcatttta	tccgtactcc	tgatgatgca	tggttactca	ccactgcgat	4350
4351	ccccgggaaa	acagcattcc	aggtattaga	agaatatcct	gattcaggtg	4400
4401	aaaatattgt	tgatgcgctg	gcagtgttcc	tgcgccggtt	gcattcgatt	4450
4451	cctgtttgta	attgtccttt	taacagcgat	cgcgtatttc	gtctcgctca	4500
4501	ggcgcaatca	cgaatgaata	acggtttggt	tgatgcgagt	gattttgatg	4550
4551	acgagcgtaa	tggctggcct	gttgaacaag	tctggaaaga	aatgcataaa	4600
4601	cttttgccat	tctcaccgga	ttcagtcgtc	actcatggtg	atttctcact	4650
4651	tgataacctt	atttttgacg	aggggaaatt	aataggttgt	attgatgttg	4700
4701	gacgagtcgg	aatcgcagac	cgataccagg	atcttgccat	cctatggaac	4750
4751	tgcctcggtg	agttttctcc	ttcattacag	aaacggcttt	ttcaaaaata	4800
4801	tggtattgat	aatcctgata	tgaataaatt	gcagtttcat	ttgatgctcg	4850
4851	atgagttttt	ctaagaatta	attcatgagc	ggatacatat	ttgaatgtat	4900
4901	ttagaaaaat	aaacaaatag	gggttccgcg	cacatttccc	cgaaaagtgc	4950
4951	cacctgaaat	tgtaaacgtt	aatattttgt	taaaattcgc	gttaaatttt	5000
5001	tgttaaatca	gctcattttt	taaccaatag	gccgaaatcg	gcaaaatccc	5050
5051	ttataaatca	aaagaataga	ccgagatagg	gttgagtgtt	gttccagttt	5100
5101	ggaacaagag	tccactatta	aagaacgtgg	actccaacgt	caaagggcga	5150
5151	aaaaccgtct	atcagggcga	tggcccacta	cgtgaaccat	caccctaatc	5200
5201	aagttttttg	gggtcgaggt	gccgtaaagc	actaaatcgg	aaccctaaag	5250
5251	ggagcccccg	atttagagct	tgacggggaa	agccggcgaa	cgtggcgaga	5300
5301	aaggaaggga	agaaagcgaa	aggagcgggc	gctagggcgc	tggcaagtgt	5350
5351	agcggtcacg	ctgcgcgtaa	ccaccacacc	cgccgcgctt	aatgcgccgc	5400
5401	tacagggcgc	gtcccattcg	cca	5423