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- 2025年11月21日
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- 详细信息
- 技术资料
- 库存：
大量
- 英文名：
pET-30c(+)
- 保质期：
1年
- 供应商：
再康生物
- 保存条件：
低温运输，-20℃保存
质粒编号: 60908-2832
质粒名称: pET-30c(+)
其他名称: pET30c, pet 30c
质粒大小（bp）: 5423
抗性: Kanamycin (卡那霉素)
质粒类型: 大肠杆菌表达载体
是否病毒: Nonviral
启动子:
稳转或瞬转: Transient
组成型或诱导性: Constitutive
表达水平: High
蛋白标签: N-His, N-S, N-Thrombin, N-EK, C-His
5′ 测序引物:T7
5′ 测序引物序列: 5′-TAATACGACTCACTATAGGG-3′
GenBank号:
其他属性: Nterm thrombin cleavage site; Nterm enterokinase cleavage site; a,b,c vary by MCS
质粒图谱:
| Feature Name | Start | End | |
|---|---|---|---|
| T7_terminator | 129 | 1 | |
| T7_Terminal_primer | 69 | 87 | |
| EK | 231 | 220 | |
| lacI | 303 | 1909 | |
| T7_transl_en_RBS | 373 | 357 | |
| lacO | 418 | 391 | |
| T7_promoter | 436 | 418 | |
| tet (300 – 563) | 472 | 735 | |
| pBRrevBam_primer | 543 | 524 | |
| tet (576 – 851) | 1968 | 2243 | |
| ROP | 2718 | 2909 | |
| pGEX_3_primer | 2925 | 2903 | |
| pBR322_origin | 3943 | 3324 | |
| KanR2 | 4049 | 4864 |
| ORF | Start | End | |
|---|---|---|---|
| ORF frame 1 | 328 | 1077 | |
| ORF frame 2 | 950 | 1909 | |
| ORF frame 2 | 4049 | 4864 |
| Enzyme Name | Cut |
|---|---|
| XhoI | 158 |
| NotI | 166 |
| EagI | 166 |
| HindIII | 173 |
| SalI | 179 |
| SacI | 190 |
| EcoRI | 192 |
| BamHI | 198 |
| EcoRV | 209 |
| NcoI | 213 |
| KpnI | 239 |
| BglII | 242 |
| BstBI | 269 |
| NdeI | 347 |
| XbaI | 385 |
| BclI | 1191 |
| ApaI | 1388 |
| HpaI | 1683 |
| FspI | 2259 |
| NruI | 4137 |
| SmaI | 4354 |
| XmaI | 4352 |
质粒序列:
| 1 | atccggatat | agttcctcct | ttcagcaaaa | aacccctcaa | gacccgttta | 50 |
| 51 | gaggccccaa | ggggttatgc | tagttattgc | tcagcggtgg | cagcagccaa | 100 |
| 101 | ctcagcttcc | tttcgggctt | tgttagcagc | cggatctcag | tggtggtggt | 150 |
| 151 | ggtggtgctc | gagtgcggcc | gcaagcttgt | cgacggagct | cgaattcgga | 200 |
| 201 | tccacagata | tcccatggcc | ttgtcgtcgt | cgtcggtacc | cagatctggg | 250 |
| 251 | ctgtccatgt | gctggcgttc | gaatttagca | gcagcggttt | ctttcatacc | 300 |
| 301 | agaaccgcgt | ggcaccagac | cagaagaatg | atgatgatga | tggtgcatat | 350 |
| 351 | gtatatctcc | ttcttaaagt | taaacaaaat | tatttctaga | ggggaattgt | 400 |
| 401 | tatccgctca | caattcccct | atagtgagtc | gtattaattt | cgcgggatcg | 450 |
| 451 | agatcgatct | cgatcctcta | cgccggacgc | atcgtggccg | gcatcaccgg | 500 |
| 501 | cgccacaggt | gcggttgctg | gcgcctatat | cgccgacatc | accgatgggg | 550 |
| 551 | aagatcgggc | tcgccacttc | gggctcatga | gcgcttgttt | cggcgtgggt | 600 |
| 601 | atggtggcag | gccccgtggc | cgggggactg | ttgggcgcca | tctccttgca | 650 |
| 651 | tgcaccattc | cttgcggcgg | cggtgctcaa | cggcctcaac | ctactactgg | 700 |
| 701 | gctgcttcct | aatgcaggag | tcgcataagg | gagagcgtcg | agatcccgga | 750 |
| 751 | caccatcgaa | tggcgcaaaa | cctttcgcgg | tatggcatga | tagcgcccgg | 800 |
| 801 | aagagagtca | attcagggtg | gtgaatgtga | aaccagtaac | gttatacgat | 850 |
| 851 | gtcgcagagt | atgccggtgt | ctcttatcag | accgtttccc | gcgtggtgaa | 900 |
| 901 | ccaggccagc | cacgtttctg | cgaaaacgcg | ggaaaaagtg | gaagcggcga | 950 |
| 951 | tggcggagct | gaattacatt | cccaaccgcg | tggcacaaca | actggcgggc | 1000 |
| 1001 | aaacagtcgt | tgctgattgg | cgttgccacc | tccagtctgg | ccctgcacgc | 1050 |
| 1051 | gccgtcgcaa | attgtcgcgg | cgattaaatc | tcgcgccgat | caactgggtg | 1100 |
| 1101 | ccagcgtggt | ggtgtcgatg | gtagaacgaa | gcggcgtcga | agcctgtaaa | 1150 |
| 1151 | gcggcggtgc | acaatcttct | cgcgcaacgc | gtcagtgggc | tgatcattaa | 1200 |
| 1201 | ctatccgctg | gatgaccagg | atgccattgc | tgtggaagct | gcctgcacta | 1250 |
| 1251 | atgttccggc | gttatttctt | gatgtctctg | accagacacc | catcaacagt | 1300 |
| 1301 | attattttct | cccatgaaga | cggtacgcga | ctgggcgtgg | agcatctggt | 1350 |
| 1351 | cgcattgggt | caccagcaaa | tcgcgctgtt | agcgggccca | ttaagttctg | 1400 |
| 1401 | tctcggcgcg | tctgcgtctg | gctggctggc | ataaatatct | cactcgcaat | 1450 |
| 1451 | caaattcagc | cgatagcgga | acgggaaggc | gactggagtg | ccatgtccgg | 1500 |
| 1501 | ttttcaacaa | accatgcaaa | tgctgaatga | gggcatcgtt | cccactgcga | 1550 |
| 1551 | tgctggttgc | caacgatcag | atggcgctgg | gcgcaatgcg | cgccattacc | 1600 |
| 1601 | gagtccgggc | tgcgcgttgg | tgcggacatc | tcggtagtgg | gatacgacga | 1650 |
| 1651 | taccgaagac | agctcatgtt | atatcccgcc | gttaaccacc | atcaaacagg | 1700 |
| 1701 | attttcgcct | gctggggcaa | accagcgtgg | accgcttgct | gcaactctct | 1750 |
| 1751 | cagggccagg | cggtgaaggg | caatcagctg | ttgcccgtct | cactggtgaa | 1800 |
| 1801 | aagaaaaacc | accctggcgc | ccaatacgca | aaccgcctct | ccccgcgcgt | 1850 |
| 1851 | tggccgattc | attaatgcag | ctggcacgac | aggtttcccg | actggaaagc | 1900 |
| 1901 | gggcagtgag | cgcaacgcaa | ttaatgtaag | ttagctcact | cattaggcac | 1950 |
| 1951 | cgggatctcg | accgatgccc | ttgagagcct | tcaacccagt | cagctccttc | 2000 |
| 2001 | cggtgggcgc | ggggcatgac | tatcgtcgcc | gcacttatga | ctgtcttctt | 2050 |
| 2051 | tatcatgcaa | ctcgtaggac | aggtgccggc | agcgctctgg | gtcattttcg | 2100 |
| 2101 | gcgaggaccg | ctttcgctgg | agcgcgacga | tgatcggcct | gtcgcttgcg | 2150 |
| 2151 | gtattcggaa | tcttgcacgc | cctcgctcaa | gccttcgtca | ctggtcccgc | 2200 |
| 2201 | caccaaacgt | ttcggcgaga | agcaggccat | tatcgccggc | atggcggccc | 2250 |
| 2251 | cacgggtgcg | catgatcgtg | ctcctgtcgt | tgaggacccg | gctaggctgg | 2300 |
| 2301 | cggggttgcc | ttactggtta | gcagaatgaa | tcaccgatac | gcgagcgaac | 2350 |
| 2351 | gtgaagcgac | tgctgctgca | aaacgtctgc | gacctgagca | acaacatgaa | 2400 |
| 2401 | tggtcttcgg | tttccgtgtt | tcgtaaagtc | tggaaacgcg | gaagtcagcg | 2450 |
| 2451 | ccctgcacca | ttatgttccg | gatctgcatc | gcaggatgct | gctggctacc | 2500 |
| 2501 | ctgtggaaca | cctacatctg | tattaacgaa | gcgctggcat | tgaccctgag | 2550 |
| 2551 | tgatttttct | ctggtcccgc | cgcatccata | ccgccagttg | tttaccctca | 2600 |
| 2601 | caacgttcca | gtaaccgggc | atgttcatca | tcagtaaccc | gtatcgtgag | 2650 |
| 2651 | catcctctct | cgtttcatcg | gtatcattac | ccccatgaac | agaaatcccc | 2700 |
| 2701 | cttacacgga | ggcatcagtg | accaaacagg | aaaaaaccgc | ccttaacatg | 2750 |
| 2751 | gcccgcttta | tcagaagcca | gacattaacg | cttctggaga | aactcaacga | 2800 |
| 2801 | gctggacgcg | gatgaacagg | cagacatctg | tgaatcgctt | cacgaccacg | 2850 |
| 2851 | ctgatgagct | ttaccgcagc | tgcctcgcgc | gtttcggtga | tgacggtgaa | 2900 |
| 2901 | aacctctgac | acatgcagct | cccggagacg | gtcacagctt | gtctgtaagc | 2950 |
| 2951 | ggatgccggg | agcagacaag | cccgtcaggg | cgcgtcagcg | ggtgttggcg | 3000 |
| 3001 | ggtgtcgggg | cgcagccatg | acccagtcac | gtagcgatag | cggagtgtat | 3050 |
| 3051 | actggcttaa | ctatgcggca | tcagagcaga | ttgtactgag | agtgcaccat | 3100 |
| 3101 | atatgcggtg | tgaaataccg | cacagatgcg | taaggagaaa | ataccgcatc | 3150 |
| 3151 | aggcgctctt | ccgcttcctc | gctcactgac | tcgctgcgct | cggtcgttcg | 3200 |
| 3201 | gctgcggcga | gcggtatcag | ctcactcaaa | ggcggtaata | cggttatcca | 3250 |
| 3251 | cagaatcagg | ggataacgca | ggaaagaaca | tgtgagcaaa | aggccagcaa | 3300 |
| 3301 | aaggccagga | accgtaaaaa | ggccgcgttg | ctggcgtttt | tccataggct | 3350 |
| 3351 | ccgcccccct | gacgagcatc | acaaaaatcg | acgctcaagt | cagaggtggc | 3400 |
| 3401 | gaaacccgac | aggactataa | agataccagg | cgtttccccc | tggaagctcc | 3450 |
| 3451 | ctcgtgcgct | ctcctgttcc | gaccctgccg | cttaccggat | acctgtccgc | 3500 |
| 3501 | ctttctccct | tcgggaagcg | tggcgctttc | tcatagctca | cgctgtaggt | 3550 |
| 3551 | atctcagttc | ggtgtaggtc | gttcgctcca | agctgggctg | tgtgcacgaa | 3600 |
| 3601 | ccccccgttc | agcccgaccg | ctgcgcctta | tccggtaact | atcgtcttga | 3650 |
| 3651 | gtccaacccg | gtaagacacg | acttatcgcc | actggcagca | gccactggta | 3700 |
| 3701 | acaggattag | cagagcgagg | tatgtaggcg | gtgctacaga | gttcttgaag | 3750 |
| 3751 | tggtggccta | actacggcta | cactagaagg | acagtatttg | gtatctgcgc | 3800 |
| 3801 | tctgctgaag | ccagttacct | tcggaaaaag | agttggtagc | tcttgatccg | 3850 |
| 3851 | gcaaacaaac | caccgctggt | agcggtggtt | tttttgtttg | caagcagcag | 3900 |
| 3901 | attacgcgca | gaaaaaaagg | atctcaagaa | gatcctttga | tcttttctac | 3950 |
| 3951 | ggggtctgac | gctcagtgga | acgaaaactc | acgttaaggg | attttggtca | 4000 |
| 4001 | tgaacaataa | aactgtctgc | ttacataaac | agtaatacaa | ggggtgttat | 4050 |
| 4051 | gagccatatt | caacgggaaa | cgtcttgctc | taggccgcga | ttaaattcca | 4100 |
| 4101 | acatggatgc | tgatttatat | gggtataaat | gggctcgcga | taatgtcggg | 4150 |
| 4151 | caatcaggtg | cgacaatcta | tcgattgtat | gggaagcccg | atgcgccaga | 4200 |
| 4201 | gttgtttctg | aaacatggca | aaggtagcgt | tgccaatgat | gttacagatg | 4250 |
| 4251 | agatggtcag | actaaactgg | ctgacggaat | ttatgcctct | tccgaccatc | 4300 |
| 4301 | aagcatttta | tccgtactcc | tgatgatgca | tggttactca | ccactgcgat | 4350 |
| 4351 | ccccgggaaa | acagcattcc | aggtattaga | agaatatcct | gattcaggtg | 4400 |
| 4401 | aaaatattgt | tgatgcgctg | gcagtgttcc | tgcgccggtt | gcattcgatt | 4450 |
| 4451 | cctgtttgta | attgtccttt | taacagcgat | cgcgtatttc | gtctcgctca | 4500 |
| 4501 | ggcgcaatca | cgaatgaata | acggtttggt | tgatgcgagt | gattttgatg | 4550 |
| 4551 | acgagcgtaa | tggctggcct | gttgaacaag | tctggaaaga | aatgcataaa | 4600 |
| 4601 | cttttgccat | tctcaccgga | ttcagtcgtc | actcatggtg | atttctcact | 4650 |
| 4651 | tgataacctt | atttttgacg | aggggaaatt | aataggttgt | attgatgttg | 4700 |
| 4701 | gacgagtcgg | aatcgcagac | cgataccagg | atcttgccat | cctatggaac | 4750 |
| 4751 | tgcctcggtg | agttttctcc | ttcattacag | aaacggcttt | ttcaaaaata | 4800 |
| 4801 | tggtattgat | aatcctgata | tgaataaatt | gcagtttcat | ttgatgctcg | 4850 |
| 4851 | atgagttttt | ctaagaatta | attcatgagc | ggatacatat | ttgaatgtat | 4900 |
| 4901 | ttagaaaaat | aaacaaatag | gggttccgcg | cacatttccc | cgaaaagtgc | 4950 |
| 4951 | cacctgaaat | tgtaaacgtt | aatattttgt | taaaattcgc | gttaaatttt | 5000 |
| 5001 | tgttaaatca | gctcattttt | taaccaatag | gccgaaatcg | gcaaaatccc | 5050 |
| 5051 | ttataaatca | aaagaataga | ccgagatagg | gttgagtgtt | gttccagttt | 5100 |
| 5101 | ggaacaagag | tccactatta | aagaacgtgg | actccaacgt | caaagggcga | 5150 |
| 5151 | aaaaccgtct | atcagggcga | tggcccacta | cgtgaaccat | caccctaatc | 5200 |
| 5201 | aagttttttg | gggtcgaggt | gccgtaaagc | actaaatcgg | aaccctaaag | 5250 |
| 5251 | ggagcccccg | atttagagct | tgacggggaa | agccggcgaa | cgtggcgaga | 5300 |
| 5301 | aaggaaggga | agaaagcgaa | aggagcgggc | gctagggcgc | tggcaagtgt | 5350 |
| 5351 | agcggtcacg | ctgcgcgtaa | ccaccacacc | cgccgcgctt | aatgcgccgc | 5400 |
| 5401 | tacagggcgc | gtcccattcg | cca | 5423 |
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pET-30c(+)
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