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- 详细信息
- 技术资料
- 保存条件:
低温运输,-20℃保存
- 保质期:
1年
- 英文名:
pECV25
- 库存:
大量
- 供应商:
信裕生物
质粒编号:
质粒名称: pECV25
其他名称:
质粒大小(bp): 11317
抗性: G418R, ampR, hygR
质粒类型: Unspecified
是否病毒: Unspecified
启动子:
稳转或瞬转: Unspecified
组成型或诱导性: Unspecified
表达水平: Unspecified
蛋白标签:
测序引物:
GenBank号:
其他属性:
The order of the major features in this plasmid is:bla (ampR) – hph (hygR) – RSV LTR – HindIII – rabbit beta-globin intron splicing signals – NotI – agpt (G418R) – XhoI – rabbit beta-globin polyadenylation signal – SalI – oriP – SacI – EBNA-1 – ClaI. [3] Shuttle expression vector maintained episomally in human cells at a copy number of 25 – 50. Shows high transfection efficiency and can be introduced as supercoiled plasmids. [3] The EBNA-1 gene contains an in-frame deletion of a repetitive region of 700 +/- 20 bp. This deletion increases the number of stably maintained plasmid molecules per cell. [3] Vector can be rescued from transfected cells with no detectable rearrangements. [3] Restriction digests of the clone give the following sizes (kb): HindIII/SacI–5.7 (doublet); SalI–11.5; XhoI–11.5. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, vertebrate cells, E.coli, HeLa cells.
contains sequence nuclear antigen 1;expression vector;shuttle vector;Shuttle expression vector maintained episomally in human cells at a copy number of 25 – 50. Shows high transfection efficiency and can be introduced as supercoiled plasmids.
质粒图谱:

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