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- 2025年07月15日
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- 详细信息
- 技术资料
- 保存条件：
4℃保存
- 保质期：
6个月
- 英文名：
EpiQuik Global Mono-Methyl Histone H3-K27 Quantification Kit (Fluorometric)
- 库存：
1
- 供应商：
上海信裕
- 通用Mono-Methyl组蛋白H3-K27定量试剂盒(Fluorometric) 详细信息：
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Product Overview
The EpiQuik™ Global Mono-Methyl Histone H3-K27 Quantification Kit (Fluorometric) is a convenient package of tools that allows the experimenter to specifically measure global mono-methylation of histone H3-K27 fluorometrically using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells.
WHY CHOOSE THE EPIQUIK™ GLOBAL MONO-METHYL HISTONE H3-K27 QUANTIFICATION KIT (FLUOROMETRIC)?
- Quick and efficient, which can be finished within 2.5 hours.
- Innovative fluorometric assay with no need for radioactivity, electrophoresis, and chromatography.
- Specifically capture mono-methylated H3-K27 with the detection limit as low as 0.5 ng/well and detection range from 20 ng-5 µg/well of histone extracts.
- The control is conveniently included for quantification of mono-methylated H3-K27.
- Strip microplate format makes the assay flexible: manual or high throughput.
- Simple, reliable, and consistent assay conditions.
通用Mono-Methyl组蛋白H3-K27定量试剂盒(Fluorometric) Principle & Procedure
The EpiQuik™ Global Mono-Methyl Histone H3-K27 Quantification Kit (Fluorometric) is designed for measuring global histone H3-K27 mono-methylation. In an assay with this kit, the mono-methylated histone H3 at lysine 27 is captured to the strip wells coated with anti-mono-methyl H3-K27 antibody. The captured mono-methylated histone H3-K27 can be then detected with a labeled detection antibody followed by fluorescent development reagent. The ratio of mono-methylated H3-K27 is proportional to the intensity of fluorescence. The absolute amount of mono-methylated H3-K27 can be quantitated by comparing to the standard control.
SCHEMATIC PROCEDURE: 
通用Mono-Methyl组蛋白H3-K27定量试剂盒(Fluorometric) Product Components
F1 (10X wash buffer)
F2 (antibody buffer)
F3 (detection antibody, 1 mg/ml)*
F4 (fluoro developer)*
F5 (fluoro enhancer)*
F6 (fluoro dilution)
Standard control (100 µg/ml)*
8 well sample strips (with frame)
8 well standard control strips
User guide
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* For maximum recovery of the products, centrifuge the original vial prior to opening the cap.通用Mono-Methyl组蛋白H3-K27定量试剂盒(Fluorometric) User Guide & MSDS
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