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- 2025年07月08日
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- 文献和实验
- 技术资料
- 保存条件：
低温运输，-20℃保存
- 保质期：
1年
- 英文名：
lambda FIX II
- 库存：
大量
- 供应商：
信裕生物
质粒编号:
质粒名称: lambda FIX II
其他名称:
质粒大小（bp）: 4300
抗性:
质粒类型: Unspecified
是否病毒:
启动子:
稳转或瞬转:
组成型或诱导性:
表达水平:
蛋白标签:
测序引物:
GenBank号: X04125, X16973
其他属性: Hosts: E.coli XL-1-Blue MRA, E.coli XL-1-Blue MRA P2. Related vectors: lambda.
质粒图谱:
质粒序列:
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文献和实验Purification of DNA from lambda phage
ml μltracentrifuged media. Alternative protocol for lambda DNA preparation: After step II A (RNase and DNase digestion)add 2.9 g NaCl and 5 g PEG-8000 to each 50 ml tube (be careful to exclude clumps of PEG-8000,which are hard to dissolve.)Shake
Using Recombineering to Generate Point Mutations: The Oligonucleotide-Based Hit and Fix Method
Ability to manipulate the genome or design genes with desired mutation is critical for functional studies. Recombineering has made genetic manipulation of large genomic fragments very feasible and efficient. In the bacteriophage lambda-based
Screening lambda cDNA or genomic libraries
ZAP II.For lambda gt11 use the host Y1090.The Barstead mixed stage C.elegans cDNA library is in the original lamda ZAP vector,and requires a host carrying the supF mutation,such as Y1090.Growing the cells:grow up 40 ml of bacteria o/n in LBspin 5 min
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