- 询价
- TIANDZ
- 中国
- 1655
- 2025年07月08日
企业认证
- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
低温运输，-20℃保存
- 保质期：
1年
- 英文名：
lambda ZAP II
- 库存：
大量
- 供应商：
信裕生物
质粒编号:
质粒名称: lambda ZAP II
其他名称:
质粒大小（bp）:
抗性:
质粒类型: Unspecified
是否病毒:
启动子:
稳转或瞬转:
组成型或诱导性:
表达水平:
蛋白标签:
测序引物:
GenBank号: X04125, X16973
其他属性: The cI857 repressor is temperature sensitive and so is inactive at 42 degrees but active at 32 degrees, allowing selection against lysogeny. Contains phagemid Bluescript SK-. Hosts: E.coli XL-1-Blue MRF'. Related vectors: lambda, lambda ZAP Express.
质粒图谱:
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文献和实验Clone Excision Methods for the Lambda ZAP-Based Vectors
The Lambda ZAP� vectors have been designed to allow in vivo excision and recircularization of the cloned insert and phagemid sequences contained within the λ vector (1 ). Once a Lambda ZAP library is constructed and amplified, putative
cDNA Library Construction for the Lambda ZAP-Based Vectors
Because the vast majority of plant viruses have a positive-sense RNA genome, which acts as the viral mRNA, the RNA must first be converted into cDNA before cloning, amplification, and subsequent manipulation. Successful cDNA synthesis
Screening lambda cDNA or genomic libraries
ZAP II.For lambda gt11 use the host Y1090.The Barstead mixed stage C.elegans cDNA library is in the original lamda ZAP vector,and requires a host carrying the supF mutation,such as Y1090.Growing the cells:grow up 40 ml of bacteria o/n in LBspin 5 min
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