Peptide sequence around phosphorylation site of serine 21 (T-S-S(p)-F-A) derived from Human GSK3a.
Product Name: GSK3a(Phospho-Ser21) Antibody
Host Species: Rabbit
Clonality: Polyclonal
Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications: WB IHC IF
Species Reactivity: Hu Ms Rt
Specificity: The antibody detects endogenous level of GSK3a only when phosphorylated at serine 21and does not detect GSK-3b when phosphorylated at Ser9.
Immunogen Type: Peptide-KLH
Immunogen Description: Peptide sequence around phosphorylation site of serine 21 (T-S-S(p)-F-A) derived from Human GSK3a.
Target Name: GSK3a
Modification: Phospho
Alternative Names: Factor A; GSK-3 alpha; kinase GSK3-alpha
Western blot analysis of extracts from 293 cells untreated(lane 1) or treated with serum(lane 2) using GSK3a(Phospho-Ser21) Antibody #11007.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3a(Phospho-Ser21) Antibody #11007(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic staining using GSK3a(Phospho-Ser21)
Western blot analysis of extracts from 293 cells, treated with insulin or calf intestinal phosphatase (CIP), using GSK3α (Phospho-Ser21) Antibody #11007.
Dan Liu, Yi Huang, Jing Zenget al,Down-regulation of JAK1 by RNA interference inhibits growth of the lung cancer cell line A549 and interferes with the PI3K/mTOR pathway., Journal of Cancer Research and Clinical Oncology, 137(11):1629-1640 (2011), PMID: 21861134
et al,Down-regulation of JAK1 by RNA interference inhibits growth of the lung cancer cell line A549 and interferes with the PI3K/mTOR pathway. In J Cancer Res Clin Oncol on 2011 Nov by Dan Liu, Yi Huang, et al..PMID:21861134,, (2011), PMID: 21861134