- 询价
- Signalway Antibody
- t505
- USA
- 2025年07月11日
- WB IF/ICC
- Mouse
- Tag
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- 详细信息
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- 文献和实验
- 技术资料
- 抗体英文名：
His-Tag Mouse Monoclonal Antibody
- 靶点：
His-Tag
- 浓度：
1.0mg/ml
- 应用范围：
WB IF/ICC
- 宿主：
Mouse
- 适应物种：
Tag
- 克隆性：
多克隆
- 保存条件：
Store at -20℃
- 形态：
liquid
- 免疫原：
A synthetic peptide HHHHHH coupled to KLH.
Purification
Affinity-chromatography
Specificity
His-Tag Mouse Monoclonal antibody detects endogenous levels of internal, C-terminal, or N-terminal 6His -tagged proteins.
Immunogen Type
Peptide-KLH
Immunogen Description
A synthetic peptide HHHHHH coupled to KLH.
SDS-PAGE MW
N/A
Formulation
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Images
IP antibody use：5ug His Mouse IgG1 per ml Lysate，WB 1:30001、untransfected 293 cell lysate2、transfected 293 cell lysate with His-tag fusion protein3、IP (untransfected 293+anti-His mAb+ Protein G agarose)4、IP (transfected 293+ normal Mouse IgG+Protein G agarose)5、IP (transfected 293+anti-His mAb+ Protein G agarose)6、Recombinant protein (E.coli)
产品详细信息请点击：His-Tag Mouse Monoclonal Antibody
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文献和实验PMID: 28628081
PKCε phosphorylates MIIP and promotes colorectal cancer metastasis through inhibition of RelA deacetylation.In Nat Commun on 2017 Oct 16 by Tao Chen, Jingjie Li ,et al..PMID: 29038521
PMID: 29038521
Purification and characterization of a novel cell-penetrating carrier similar to cholera toxin chimeric protein.In Protein Expr Purif on 2017 Jan by Weiping Lin, Xi Zheng ,et al..PMID: 27234002
PMID: 27234002
Suppression of p21Rac Signaling and Increased Innate Immunity Mediate Remission in Crohn’s Disease.In sci Transl Med on 2014 Apr 23 by Kaushal Parikh , Lu Zhou et al..PMID:24760188
PMID:24760188
An alternative pathway for the formation of aromatic aroma compounds derived from l-phenylalanIne via phenylpyruvic acid in tea (Camellia sInensis (L.) O. Kuntze) leaves. In Food Chem on 2019 Jan 1 by Wang X, Zeng L, et al..PMID:30174031
PMID:30174031
PAK4 Phosphorylates Fumarase and Blocks TGFβ-Induced Cell Growth Arrest in Lung Cancer Cells. In Cancer Res on 2019 Apr 1 by Chen T, Wang T, et al..PMID:30683654
PMID:30683654
O-GlcNAcylation of PFKFB3 is required for tumor cell proliferation under hypoxia.In Oncogenesis on 2020 Feb 14，by Lei Y, Chen T, et al..PMID:32060258
PMID:32060258
Upregulation of ZNF148 in SDHB-deficient gastrointestinal stromal tumor potentiates Forkhead box M1-mediated transcription and promotes tumor cell invasion.
In Cancer Sci on 2020 Apr by Gao X, Ma C, et al..PMID: 32060966
PMID: 32060966
Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
指仅对一种抗原决定基的纯粹的抗体。这类抗体不可能通过将抗原 胞的 Hybridoma，在试管内制成了纯粹的单克隆抗体。用某种抗原使动物产生免疫感应，对应于抗原之不同部分可制成各种不同的抗体复合体。然而，因为一个产生抗体的细胞，只能产生一种抗体，所以骨髓肿瘤细胞与产生抗体细胞间的细胞杂种，可形成边产生抗体边增殖的 hybridoma，将每个杂种细胞进行克隆（无性繁殖系）培养，则一个克隆的细胞群只由单种类产生同一抗体的细胞所组成，这样得到的就是单克隆抗体。此抗体在测定
Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
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