- 询价
- Signalway Antibody
- 11002
- USA
- 2026年08月12日
- WB IHC IF
- Rabbit
- Hu Ms Rt
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- 文献和实验
- 技术资料
- 抗体英文名：
GSK3β (Phospho-Ser9) Antibody
- 靶点：
GSK3β
- 浓度：
1.0mg/ml
- 应用范围：
WB IHC IF
- 宿主：
Rabbit
- 适应物种：
Hu Ms Rt
- 克隆性：
Polyclonal
- 保存条件：
Store at -20℃
- 形态：
liquid
- 免疫原：
Peptide sequence around phosphorylation site of serine 9 (T-T-S(p)-F-A) derived from Human GSK3β .
GSK3β(Phospho-Ser9) Antibody
Host Species：
Rabbit
Clonality：
Polyclonal
Purification：
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications：
WB IHC IF
Species Reactivity：
Hu Ms Rt
Specificity：
The antibody detects endogenous level of GSK3 beta only when phosphorylated at serine 9.
Immunogen Type：
Peptide-KLH
Immunogen Description：
Peptide sequence around phosphorylation site of serine 9 (T-T-S(p)-F-A) derived from Human GSK3β.
Target Name：
GSK3β
Modification：
Phospho
Alternative Names ：
Factor A, GSK-3 beta, Protein kinase GSK-3-beta, kinase GSK-3 beta
Accession No. ：
Swiss-Prot: P49841
NCBI Protein: NP_001139628.1
SDS-PAGE MW :
46kd
Concentration :
1.0mg/ml
Images
Western blot analysis of extracts from 293 cells untreated(lane 1) or treated with serum(lane 2) using GSK3β(Phospho-Ser9) Antibody #11002.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using GSK3β (Phospho-Ser9) Antibody #11002 (left) or the same antibody preincubated with blocking peptide #51002 (right).
Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic staining using GSK3β(Phospho-Ser9) Antibody #11002.
Immunohistochemical analysis of paraffin-embedded human Lung carcinoma tissue using GSK3β (Phospho-Ser9) Antibody #11002.
Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic staining using GSK3β (Phospho-Ser9) Antibody #11002.
Western blot analysis of extracts from Hela cells, treated with EGF or calf intestinal phosphatase (CIP), using GSK3β (Phospho-Ser9) Antibody #11002.
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文献和实验下调不能说明活性降低，因为决定GSK3-beta的活性的还与其磷酸化的比例和磷酸化的位点有关。所以你除了要做GSK3-beta总的蛋白表达之外，还要做磷酸化的GSK3-beta的蛋白表达。 （2）应该活性形式和非活性形式都做。一般来讲，GSK3-beta在Ser9位点磷酸化之后活性收到抑制，而在216位点磷酸化之后，其活性收到加强。因此建议将GSK3-beta的两个磷酸化位点都做了，另外还要同时检测GSK3-beta的总蛋白表达，这样才能全面的说明问题。
，并在猴、鸡以及斑马鱼等多种样本中验证有效，全面兼容各类细胞、组织样本及实验模型。在应用性能上，该抗体经多种关键技术验证，包括Western Blot（WB）、免疫组化（IHC）、免疫荧光（IF）和流式细胞术（FCM），均表现出色，呈现清晰特异的信号与洁净的背景，有力保障实验数据的可靠性与可重复性。 产品展示 1.K200058M Anti-β-Actin Monoclonal antibody 抗体类型：鼠单抗 验证图片： 2.K101527P Anti-β-Actin Polyclonal
Optimized Protocol to Make Phospho-Specific Antibodies that Work
, not simply its level of expression. In this review, we will discuss both the design of the phosphopeptide immunogen and immunization. The affinity purification of the phospho-specific antibody as well as the methods most suitable for characterizing
