- 询价
- KLSW
- 美国 addgene
- KL-ZL-0159
- 2025年10月15日
9 年钻石会员
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 技术资料
- 保存条件：
负20度
- 保质期：
2年
- 英文名：
pCOLADuet-1
- 库存：
100
- 供应商：
kelei-bio
- 规格：
20μl
基本信息
启动子: T7/Lac
复制子: ColA ori
终止子: T7 terminator
质粒分类: 大肠杆菌载体；双表达框质粒
质粒大小: 3719bp
质粒标签: N-6×His,C-S
原核抗性: 卡那青霉素Kan
克隆菌株: DH5α
培养条件: 37℃，有氧，LB
表达宿主: BL21(DE3)
诱导方式: IPTG或乳糖及其类似物
5'测序引物: ACYCDuetUP1：GGATCTCGACGCTCTCCCT DuetUP2:TTGTACACGGCCGCATAATC
3'测序引物: DuetDOWN1:GATTAGCGGCCGTGTACAA T7-ter:TGCTAGTTATTGCTCAGCGG
备注: 同时表达两个外源蛋白
质粒简介
pCOLADuet-1 is designed for the coexpression of two target genes from a single plasmid. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (rbs). MCS-1 encodes the six-amino acid His•Tag sequence for the creation of a N-terminal fusion and MCS2 encodes the 15 amino acid S•Tag™ peptide after the last restriction site for the creation of a C-terminal fusion if desired. Genes inserted into MCS-1 can be sequenced using the ACYCDuetUP1 Primer and DuetDOWN1 Primer. Genes inserted into MCS-2 can be sequenced using the DuetUP2 Primer and T7 Terminator Primer. The vector has the COLA replicon from ColA(1) and the kanamycin resistance gene. This vector can be transformed into the same cell with plasmids containing compatible origins of replication and drug resistance genes for coexpression of up to 8 target genes.
启动子: T7/Lac
复制子: ColA ori
终止子: T7 terminator
质粒分类: 大肠杆菌载体；双表达框质粒
质粒大小: 3719bp
质粒标签: N-6×His,C-S
原核抗性: 卡那青霉素Kan
克隆菌株: DH5α
培养条件: 37℃，有氧，LB
表达宿主: BL21(DE3)
诱导方式: IPTG或乳糖及其类似物
5'测序引物: ACYCDuetUP1：GGATCTCGACGCTCTCCCT DuetUP2:TTGTACACGGCCGCATAATC
3'测序引物: DuetDOWN1:GATTAGCGGCCGTGTACAA T7-ter:TGCTAGTTATTGCTCAGCGG
备注: 同时表达两个外源蛋白
质粒简介
pCOLADuet-1 is designed for the coexpression of two target genes from a single plasmid. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (rbs). MCS-1 encodes the six-amino acid His•Tag sequence for the creation of a N-terminal fusion and MCS2 encodes the 15 amino acid S•Tag™ peptide after the last restriction site for the creation of a C-terminal fusion if desired. Genes inserted into MCS-1 can be sequenced using the ACYCDuetUP1 Primer and DuetDOWN1 Primer. Genes inserted into MCS-2 can be sequenced using the DuetUP2 Primer and T7 Terminator Primer. The vector has the COLA replicon from ColA(1) and the kanamycin resistance gene. This vector can be transformed into the same cell with plasmids containing compatible origins of replication and drug resistance genes for coexpression of up to 8 target genes.
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
技术资料暂无技术资料 索取技术资料
pCOLADuet-1质粒
询价
