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- 详细信息
- 技术资料
- 保存条件:
负20度
- 保质期:
2年
- 英文名:
pDsRed2-ER
- 库存:
100
- 供应商:
kelei
- 规格:
20μl
基本信息
启动子: CMV
复制子: pUC ori,f1 ori
终止子: SV40 poly(A) signal
质粒分类: 哺乳细胞,荧光蛋白报告载体
质粒大小: 4757bp
原核抗性: Kan 筛选标记: Neo
克隆菌株: DH5α
培养条件: 37℃,有氧 LB
表达宿主: 哺乳细胞
5'测序引物: CMV-F:CGCAAATGGGCGGTAGGCGTG
3'测序引物: Sv40-polyA-R:GAAATTTGTGATGCTATTGC
质粒简介
pDsRed2-ER is a mammalian expression vector designed to label the endoplasmic reticulum in living cells. The vector encodes a fusion consisting of Discosoma sp. red fluorescent protein (DsRed2); the endoplasmic reticulum (ER) targeting sequence of calreticulin, fused to the 5' end of DsRed2; and the ER retention sequence, KDEL, fused to the 3' end of DsRed2. DsRed2 is a human codon-optimized variant of wild-type DsRed that has been engineered for faster maturation and lower non-specific aggregation.
启动子: CMV
复制子: pUC ori,f1 ori
终止子: SV40 poly(A) signal
质粒分类: 哺乳细胞,荧光蛋白报告载体
质粒大小: 4757bp
原核抗性: Kan 筛选标记: Neo
克隆菌株: DH5α
培养条件: 37℃,有氧 LB
表达宿主: 哺乳细胞
5'测序引物: CMV-F:CGCAAATGGGCGGTAGGCGTG
3'测序引物: Sv40-polyA-R:GAAATTTGTGATGCTATTGC
质粒简介
pDsRed2-ER is a mammalian expression vector designed to label the endoplasmic reticulum in living cells. The vector encodes a fusion consisting of Discosoma sp. red fluorescent protein (DsRed2); the endoplasmic reticulum (ER) targeting sequence of calreticulin, fused to the 5' end of DsRed2; and the ER retention sequence, KDEL, fused to the 3' end of DsRed2. DsRed2 is a human codon-optimized variant of wild-type DsRed that has been engineered for faster maturation and lower non-specific aggregation.
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