| 出品公司: | ZYbscience |
|---|---|
| 载体名称: | pCAMBIA1381Z |
| 质粒类型: | 植物载体；农杆菌双元表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | lac |
| 载体大小: | 11227 bp |
| 5' 测序引物及序列: | M13 Reverse:CAGGAAACAGCTATGAC |
| 3' 测序引物及序列: | M13/pUC Forward:CCCAGTCACGACGTTGTAAAACG |
| 载体标签: | GUS、6xHis |
| 载体抗性: | 卡那霉素 |
| 筛选标记: | 潮霉素 |
| 克隆菌株: | HB101等菌株 |
| 宿主细胞（系）: | 植物细胞、农杆菌 |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
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- 详细信息
- 技术资料
- 保存条件：
-20℃低温保存
- 保质期：
三年
- 英文名：
pCambia1381Z
- 库存：
20
- 供应商：
泽叶生物
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCambia1381Z 11226 bp DNA circular SYN 24-APR-2000
DEFINITION Binary vector pCAMBIA-1381Z, complete sequence.
ACCESSION AF234306
VERSION AF234306.1 GI:7638107
KEYWORDS .
SOURCE Binary vector pCAMBIA-1381Z
ORGANISM Binary vector pCAMBIA-1381Z
other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
REFERENCE 1 (sites)
AUTHORS Hajdukiewicz,P., Svab,Z. and Maliga,P.
TITLE The small, versatile pPZP family of Agrobacterium binary vectors
for plant transformation
JOURNAL Plant Mol. Biol. 25 (6), 989-994 (1994)
PUBMED 7919218
REFERENCE 2 (bases 1 to 11226)
AUTHORS Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W.,
Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L.,
Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z.,
Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A.
TITLE A comprehensive set of modular vectors for advanced manipulations
and efficient transformation of plants
JOURNAL Unpublished
REMARK Full description of constructs
REFERENCE 3 (bases 1 to 11226)
AUTHORS Roberts,C., Rajagopal,S., Smith,L.M., Nguyen,T.A., Yang,W.,
Nugrohu,S., Ravi,K.S., Vijayachandra,K., Harcourt,R.L.,
Dransfield,L., Desamero,N., Slamet,I., Hadjukiewicz,P., Svab,Z.,
Maliga,P., Mayer,J.E., Keese,P.K., Kilian,A. and Jefferson,R.A.
TITLE Direct Submission
JOURNAL Submitted (15-FEB-2000) CAMBIA, Clunies Ross St, Black Mountain /
GPO Box 3200, Canberra, ACT 2601, Australia
FEATURES Location/Qualifiers
source 1..11226
/organism="Binary vector pCAMBIA-1381Z"
/mol_type="other DNA"
/db_xref="taxon:118398"
CDS join(2..16,207..2054)
/codon_start=1
/product="GUSA, hexaHis tagged"
/protein_id="AAF65372.1"
/db_xref="GI:7638111"
/translation="MVDLRNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWE
SALQESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHY
GKVWVNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDE
NGKKKQSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANG
DVSVELRDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELEVTAKSQTESDIYPLR
VGIRSVAVKGQQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSY
RTSHYPYAEEMLDWADEHGIVVIDETAAVGFQLSLGIGFEAGNKPKELYSEEAVNGET
QQAHLQAIKELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPIT
CVNVMFCDAHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPI
IITEYGVDTLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQ
GILRVGGNKKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQASHHHHHHV"
exon 2..16
/note="synthetic first gus exon (modular)"
intron 17..206
/note="Castorbean catalase intron; modified"
exon 207..2054
/note="second gusA exon, His6"
misc_feature 2086..2338
/note="nos (nopaline synthase) 3'UTR (polyA signal)"
misc_feature 2376..2401
/note="right border T-DNA repeat"
misc_feature complement(3442..4442)
/note="STA region from pVS1 plasmid"
rep_origin complement(5035..6035)
/note="pVS1-REP; replication origin from pVS1"
misc_feature complement(6445..6705)
/note="bom site from pBR322"
rep_origin complement(6845..7125)
/note="pBR322 origin of replication"
CDS complement(7416..8210)
/note="aadA (kanamycin resistance) gene amplified from
pIG121Hm"
/codon_start=1
/product="aminoglycoside phosphotransferase"
/protein_id="AAF65369.1"
/db_xref="GI:7638108"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
misc_feature 8635..8660
/note="left border repeat from C58 T-DNA"
misc_feature 8727..8935
/note="CaMV 3'UTR (polyA signal)"
CDS complement(8951..9976)
/note="hptII (hygromycin resistance) gene"
/codon_start=1
/product="hygromycin phosphotransferase"
/protein_id="AAF65370.1"
/db_xref="GI:7638109"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR
GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD
LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY
HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS
EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ
SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS
TRPRAKK"
promoter complement(10012..10792)
/note="CaMV35S2; CaMV 35S promoter, duplicated"
promoter 10927..10981
/note="PlacZ; lacZ promoter"
CDS 11009..>11207
/codon_start=1
/product="LacZ alpha, truncated"
/protein_id="AAF65371.1"
/db_xref="GI:7638110"
/translation="MTMITNSRGSVDLQPSLALAVVLQRRDWENPGVTQLNRLAAHPP
FASWRNSEEARTDRPSQQLRSL"
misc_feature 11023..11058
/note="pUC8 MCS; polylinker"
ORIGIN
1 catggtagat ctgagggtaa atttctagtt tttctccttc attttcttgg ttaggaccct
61 tttctctttt tatttttttg agctttgatc tttctttaaa ctgatctatt ttttaattga
121 ttggttatgg tgtaaatatt acatagcttt aactgataat ctgattactt tatttcgtgt
181 gtctatgatg atgatgatag ttacagaacc gacgactcgt ccgtcctgta gaaaccccaa
241 cccgtgaaat caaaaaactc gacggcctgt gggcattcag tctggatcgc gaaaactgtg
301 gaattgatca gcgttggtgg gaaagcgcgt tacaagaaag ccgggcaatt gctgtgccag
361 gcagttttaa cgatcagttc gccgatgcag atattcgtaa ttatgcgggc aacgtctggt
421 atcagcgcga agtctttata ccgaaaggtt gggcaggcca gcgtatcgtg ctgcgtttcg
481 atgcggtcac tcattacggc aaagtgtggg tcaataatca ggaagtgatg gagcatcagg
541 gcggctatac gccatttgaa gccgatgtca cgccgtatgt tattgccggg aaaagtgtac
601 gtatcaccgt ttgtgtgaac aacgaactga actggcagac tatcccgccg ggaatggtga
661 ttaccgacga aaacggcaag aaaaagcagt cttacttcca tgatttcttt aactatgccg
721 gaatccatcg cagcgtaatg ctctacacca cgccgaacac ctgggtggac gatatcaccg
781 tggtgacgca tgtcgcgcaa gactgtaacc acgcgtctgt tgactggcag gtggtggcca
841 atggtgatgt cagcgttgaa ctgcgtgatg cggatcaaca ggtggttgca actggacaag
901 gcactagcgg gactttgcaa gtggtgaatc cgcacctctg gcaaccgggt gaaggttatc
961 tctatgaact cgaagtcaca gccaaaagcc agacagagtc tgatatctac ccgcttcgcg
1021 tcggcatccg gtcagtggca gtgaagggcc aacagttcct gattaaccac aaaccgttct
1081 actttactgg ctttggtcgt catgaagatg cggacttacg tggcaaagga ttcgataacg
1141 tgctgatggt gcacgaccac gcattaatgg actggattgg ggccaactcc taccgtacct
1201 cgcattaccc ttacgctgaa gagatgctcg actgggcaga tgaacatggc atcgtggtga
1261 ttgatgaaac tgctgctgtc ggctttcagc tgtctttagg cattggtttc gaagcgggca
1321 acaagccgaa agaactgtac agcgaagagg cagtcaacgg ggaaactcag caagcgcact
1381 tacaggcgat taaagagctg atagcgcgtg acaaaaacca cccaagcgtg gtgatgtgga
1441 gtattgccaa cgaaccggat acccgtccgc aaggtgcacg ggaatatttc gcgccactgg
1501 cggaagcaac gcgtaaactc gacccgacgc gtccgatcac ctgcgtcaat gtaatgttct
1561 gcgacgctca caccgatacc atcagcgatc tctttgatgt gctgtgcctg aaccgttatt
1621 acggatggta tgtccaaagc ggcgatttgg aaacggcaga gaaggtactg gaaaaagaac
1681 ttctggcctg gcaggagaaa ctgcatcagc cgattatcat caccgaatac ggcgtggata
1741 cgttagccgg gctgcactca atgtacaccg acatgtggag tgaagagtat cagtgtgcat
1801 ggctggatat gtatcaccgc gtctttgatc gcgtcagcgc cgtcgtcggt gaacaggtat
1861 ggaatttcgc cgattttgcg acctcgcaag gcatattgcg cgttggcggt aacaagaaag
1921 ggatcttcac tcgcgaccgc aaaccgaagt cggcggcttt tctgctgcaa aaacgctgga
1981 ctggcatgaa cttcggtgaa aaaccgcagc agggaggcaa acaagctagc caccaccacc
2041 accaccacgt gtgaattaca ggtgaccagc tcgaatttcc ccgatcgttc aaacatttgg
2101 caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt
2161 ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
2221 tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata
2281 tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa
2341 ttaaactatc agtgtttgac aggatatatt ggcgggtaaa cctaagagaa aagagcgttt
2401 attagaataa cggatattta aaagggcgtg aaaaggttta tccgttcgtc catttgtatg
2461 tgcatgccaa ccacagggtt cccctcggga tcaaagtact ttgatccaac ccctccgctg
2521 ctatagtgca gtcggcttct gacgttcagt gcagccgtct tctgaaaacg acatgtcgca
2581 caagtcctaa gttacgcgac aggctgccgc cctgcccttt tcctggcgtt ttcttgtcgc
2641 gtgttttagt cgcataaagt agaatacttg cgactagaac cggagacatt acgccatgaa
2701 caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc agcaccgacg accaggactt
2761 gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc aagctgtttt ccgagaagat
2821 caccggcacc aggcgcgacc gcccggagct ggccaggatg cttgaccacc tacgccctgg
2881 cgacgttgtg acagtgacca ggctagaccg cctggcccgc agcacccgcg acctactgga
2941 cattgccgag cgcatccagg aggccggcgc gggcctgcgt agcctggcag agccgtgggc
3001 cgacaccacc acgccggccg gccgcatggt gttgaccgtg ttcgccggca ttgccgagtt
3061 cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc gaggccgcca aggcccgagg
3121 cgtgaagttt ggcccccgcc ctaccctcac cccggcacag atcgcgcacg cccgcgagct
3181 gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca ctgcttggcg tgcatcgctc
3241 gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg cccaccgagg ccaggcggcg
3301 cggtgccttc cgtgaggacg cattgaccga ggccgacgcc ctggcggccg ccgagaatga
3361 acgccaagag gaacaagcat gaaaccgcac caggacggcc aggacgaacc gtttttcatt
3421 accgaagaga tcgaggcgga gatgatcgcg gccgggtacg tgttcgagcc gcccgcgcac
3481 gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt ctgatgccaa gctggcggcc
3541 tggccggcca gcttggccgc tgaagaaacc gagcgccgcc gtctaaaaag gtgatgtgta
3601 tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata tgatgcgatg agtaaataaa
3661 caaatacgca aggggaacgc atgaaggtta tcgctgtact taaccagaaa ggcgggtcag
3721 gcaagacgac catcgcaacc catctagccc gcgccctgca actcgccggg gccgatgttc
3781 tgttagtcga ttccgatccc cagggcagtg cccgcgattg ggcggccgtg cgggaagatc
3841 aaccgctaac cgttgtcggc atcgaccgcc cgacgattga ccgcgacgtg aaggccatcg
3901 gccggcgcga cttcgtagtg atcgacggag cgccccaggc ggcggacttg gctgtgtccg
3961 cgatcaaggc agccgacttc gtgctgattc cggtgcagcc aagcccttac gacatatggg
4021 ccaccgccga cctggtggag ctggttaagc agcgcattga ggtcacggat ggaaggctac
4081 aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg catcggcggt gaggttgccg
4141 aggcgctggc cgggtacgag ctgcccattc ttgagtcccg tatcacgcag cgcgtgagct
4201 acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc agaacccgag ggcgacgctg
4261 cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa actcatttga gttaatgagg
4321 taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc ggccgtccga gcgcacgcag
4381 cagcaaggct gcaacgttgg ccagcctggc agacacgcca gccatgaagc gggtcaactt
4441 tcagttgccg gcggaggatc acaccaagct gaagatgtac gcggtacgcc aaggcaagac
4501 cattaccgag ctgctatctg aatacatcgc gcagctacca gagtaaatga gcaaatgaat
4561 aaatgagtag atgaatttta gcggctaaag gaggcggcat ggaaaatcaa gaacaaccag
4621 gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg cggttggcca ggcgtaagcg
4681 gctgggttgt ctgccggccc tgcaatggca ctggaacccc caagcccgag gaatcggcgt
4741 gacggtcgca aaccatccgg cccggtacaa atcggcgcgg cgctgggtga tgacctggtg
4801 gagaagttga aggccgcgca ggccgcccag cggcaacgca tcgaggcaga agcacgcccc
4861 ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag aatcccggca accgccggca
4921 gccggtgcgc cgtcgattag gaagccgccc aagggcgacg agcaaccaga ttttttcgtt
4981 ccgatgctct atgacgtggg cacccgcgat agtcgcagca tcatggacgt ggccgttttc
5041 cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc gctacgagct tccagacggg
5101 cacgtagagg tttccgcagg gccggccggc atggccagtg tgtgggatta cgacctggta
5161 ctgatggcgg tttcccatct aaccgaatcc atgaaccgat accgggaagg gaagggagac
5221 aagcccggcc gcgtgttccg tccacacgtt gcggacgtac tcaagttctg ccggcgagcc
5281 gatggcggaa agcagaaaga cgacctggta gaaacctgca ttcggttaaa caccacgcac
5341 gttgccatgc agcgtacgaa gaaggccaag aacggccgcc tggtgacggt atccgagggt
5401 gaagccttga ttagccgcta caagatcgta aagagcgaaa ccgggcggcc ggagtacatc
5461 gagatcgagc tagctgattg gatgtaccgc gagatcacag aaggcaagaa cccggacgtg
5521 ctgacggttc accccgatta ctttttgatc gatcccggca tcggccgttt tctctaccgc
5581 ctggcacgcc gcgccgcagg caaggcagaa gccagatggt tgttcaagac gatctacgaa
5641 cgcagtggca gcgccggaga gttcaagaag ttctgtttca ccgtgcgcaa gctgatcggg
5701 tcaaatgacc tgccggagta cgatttgaag gaggaggcgg ggcaggctgg cccgatccta
5761 gtcatgcgct accgcaacct gatcgagggc gaagcatccg ccggttccta atgtacggag
5821 cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc gaaaaggtct ctttcctgtg
5881 gatagcacgt acattgggaa cccaaagccg tacattggga accggaaccc gtacattggg
5941 aacccaaagc cgtacattgg gaaccggtca cacatgtaag tgactgatat aaaagagaaa
6001 aaaggcgatt tttccgccta aaactcttta aaacttatta aaactcttaa aacccgcctg
6061 gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc tgcaaaaagc gcctaccctt
6121 cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc ctatcgcggc cgctggccgc
6181 tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc gcggacaagc cgcgccgtcg
6241 ccactcgacc gccggcgccc acatcaaggc accctgcctc gcgcgtttcg gtgatgacgg
6301 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
6361 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc
6421 catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag
6481 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
6541 aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
6601 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
6661 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
6721 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
6781 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
6841 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
6901 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
6961 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
7021 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
7081 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
7141 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc
7201 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
7261 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
7321 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
7381 tcacgttaag ggattttggt catgcattct aggtactaaa acaattcatc cagtaaaata
7441 taatatttta ttttctccca atcaggcttg atccccagta agtcaaaaaa tagctcgaca
7501 tactgttctt ccccgatatc ctccctgatc gaccggacgc agaaggcaat gtcataccac
7561 ttgtccgccc tgccgcttct cccaagatca ataaagccac ttactttgcc atctttcaca
7621 aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc gggcttttcc
7681 gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc ttcccagttt
7741 tcgcaatcca catcggccag atcgttattc agtaagtaat ccaattcggc taagcggctg
7801 tctaagctat tcgtataggg acaatccgat atgtcgatgg agtgaaagag cctgatgcac
7861 tccgcataca gctcgataat cttttcaggg ctttgttcat cttcatactc ttccgagcaa
7921 aggacgccat cggcctcact catgagcaga ttgctccagc catcatgccg ttcaaagtgc
7981 aggacctttg gaacaggcag ctttccttcc agccatagca tcatgtcctt ttcccgttcc
8041 acatcatagg tggtcccttt ataccggctg tccgtcattt ttaaatatag gttttcattt
8101 tctcccacca gcttatatac cttagcagga gacattcctt ccgtatcttt tacgcagcgg
8161 tatttttcga tcagtttttt caattccggt gatattctca ttttagccat ttattatttc
8221 cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac aagacgaact
8281 ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt
8341 tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa ccgcggtgat
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pCambia1381Z载体
¥1800
