| 出品公司: | |
|---|---|
| 别名: | pET23c, pet 23c |
| 质粒类型: | 大肠杆菌表达 |
| 表达水平: | 高 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 3664bp |
| 5' 测序引物: | T7 |
| 5' 测序引物序列: | 5'-TAATACGACTCACTATAGGG-3' |
| 载体标签: | N-T7, C-His |
| 载体抗性: | Ampicillin |
| 克隆菌株: | DH5a |
| 表达菌株: | BL21系列 |
| 备注: |
Same as pET21abcd(+) but no lac; a,b,c,d vary by MCS.
|
| 产品目录号: | ZY69747-3 |
| 稳定性: | 稳表达 |
| 组成型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃低温保存
- 保质期：
三年
- 英文名：
pET-23c(+)
- 库存：
20
- 供应商：
泽叶生物
pET23c载体基本信息
pET23c载体质粒图谱和多克隆位点信息
The maps for pET-23b(+), pET-23c(+) and pET-23d(+) are the same as pET-23a(+) (shown) with the following exceptions: pET-23b(+) is a 3665bp plasmid; subtract 1bp from each site beyond BamH I at 198. pET-23c(+) is a 3664bp plasmid; subtract 2bp from each site beyond BamH I at 198. pET-23d(+) is a 3663bp plasmid; the BamH I site is in the same reading frame as in pET-23c(+). An Nco I site is substituted for the Nde I site with a net 1bp deletion at position 238 of pET-23c(+). As a result, Nco I cuts pET-23d(+) at 234, and Nhe I cuts at 229. For the rest of the sites, subtract 3bp from each site beyond position 239 in pET-23a(+). Nde I does not cut pET-23d(+). Note also that Sty I is not unique in pET-23d(+).
pET23c载体简介
The pET-23a-d(+) vectors carry an N-terminal T7•Tag® sequence plus an optional C-terminal His•Tag® sequence. These vectors differ from pET-21a-d(+) by the “plain” T7 promoter instead of the T7lac promoter and by the absence of the lacI gene. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, single-stranded sequencing should be performed using the T7 terminator primer. 
pET23c载体序列
LOCUS pET-23c(+) 3664 bp DNA circular 11-SEP-2008
SOURCE
ORGANISM
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTOAUTHORAD1|Novagen Inc.|
FEATURES Location/Qualifiers
promoter complement(301..317)
/vntifkey="30"
/label=T7\promoter
protein_bind complement(205..237)
/vntifkey="31"
/label=T7\tag
terminator complement(26..72)
/vntifkey="43"
/label=T7\terminator
protein_bind complement(140..157)
/vntifkey="31"
/label=His\tag
rep_origin complement(1448..1448)
/vntifkey="33"
/label=ColE1\origin
CDS complement(2209..3066)
/vntifkey="4"
/label=bla(amp)\sequence
rep_origin complement(3198..3653)
/vntifkey="33"
/label=f1\origin
BASE COUNT 919 a 935 c 925 g 885 t
ORIGIN
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
181 cgacggagct cgaattcgga tccgacccat ttgctgtcca ccagtcatgc tagccatatg
241 tatatctcct tcttaaagtt aaacaaaatt atttctagag ggaaaccgtt gtggtctccc
301 tatagtgagt cgtattaatt tcgcgggatc gagatctcgg gcagcgttgg gtcctggcca
361 cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc taggctggcg gggttgcctt
421 actggttagc agaatgaatc accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa
481 acgtctgcga cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg
541 gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc aggatgctgc
601 tggctaccct gtggaacacc tacatctgta ttaacgaagc gctggcattg accctgagtg
661 atttttctct ggtcccgccg catccatacc gccagttgtt taccctcaca acgttccagt
721 aaccgggcat gttcatcatc agtaacccgt atcgtgagca tcctctctcg tttcatcggt
781 atcattaccc ccatgaacag aaatccccct tacacggagg catcagtgac caaacaggaa
841 aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct tctggagaaa
901 ctcaacgagc tggacgcgga tgaacaggca gacatctgtg aatcgcttca cgaccacgct
961 gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
1021 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
1081 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt
1141 agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag
1201 tgcaccatat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
1261 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
1321 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
1381 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
1441 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
1501 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
1561 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
1621 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
1681 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
1741 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
1801 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
1861 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
1921 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
1981 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
2041 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
2101 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
2161 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
2221 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
2281 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
2341 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
2401 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
2461 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
2521 tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
2581 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
2641 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
2701 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
2761 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
2821 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
2881 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
2941 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
3001 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
3061 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
3121 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
3181 ccgaaaagtg ccacctgaaa ttgtaaacgt taatattttg ttaaaattcg cgttaaattt
3241 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
3301 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
3361 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
3421 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
3481 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
3541 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
3601 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc
3661 gcca
SOURCE
ORGANISM
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTOAUTHORAD1|Novagen Inc.|
FEATURES Location/Qualifiers
promoter complement(301..317)
/vntifkey="30"
/label=T7\promoter
protein_bind complement(205..237)
/vntifkey="31"
/label=T7\tag
terminator complement(26..72)
/vntifkey="43"
/label=T7\terminator
protein_bind complement(140..157)
/vntifkey="31"
/label=His\tag
rep_origin complement(1448..1448)
/vntifkey="33"
/label=ColE1\origin
CDS complement(2209..3066)
/vntifkey="4"
/label=bla(amp)\sequence
rep_origin complement(3198..3653)
/vntifkey="33"
/label=f1\origin
BASE COUNT 919 a 935 c 925 g 885 t
ORIGIN
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
181 cgacggagct cgaattcgga tccgacccat ttgctgtcca ccagtcatgc tagccatatg
241 tatatctcct tcttaaagtt aaacaaaatt atttctagag ggaaaccgtt gtggtctccc
301 tatagtgagt cgtattaatt tcgcgggatc gagatctcgg gcagcgttgg gtcctggcca
361 cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc taggctggcg gggttgcctt
421 actggttagc agaatgaatc accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa
481 acgtctgcga cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg
541 gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc aggatgctgc
601 tggctaccct gtggaacacc tacatctgta ttaacgaagc gctggcattg accctgagtg
661 atttttctct ggtcccgccg catccatacc gccagttgtt taccctcaca acgttccagt
721 aaccgggcat gttcatcatc agtaacccgt atcgtgagca tcctctctcg tttcatcggt
781 atcattaccc ccatgaacag aaatccccct tacacggagg catcagtgac caaacaggaa
841 aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct tctggagaaa
901 ctcaacgagc tggacgcgga tgaacaggca gacatctgtg aatcgcttca cgaccacgct
961 gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
1021 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
1081 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt
1141 agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag
1201 tgcaccatat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
1261 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
1321 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
1381 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
1441 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
1501 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
1561 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
1621 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
1681 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
1741 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
1801 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
1861 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
1921 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
1981 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
2041 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
2101 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
2161 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
2221 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
2281 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
2341 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
2401 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
2461 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
2521 tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
2581 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
2641 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
2701 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
2761 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
2821 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
2881 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
2941 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
3001 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
3061 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
3121 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
3181 ccgaaaagtg ccacctgaaa ttgtaaacgt taatattttg ttaaaattcg cgttaaattt
3241 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
3301 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
3361 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
3421 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
3481 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
3541 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
3601 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc
3661 gcca
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文献和实验相关实验
表达载体的构建是生物技术和所需蛋白质产生的基本工具，本文总结了pET-32α（+）载体技术的构建，该技术通常用于研究实验室。该方法包括获得用于构建载体的外源DNA片段，将DNA片段亚克隆到pET-32α（+）表达载体中，在大肠杆菌 BL21（DE3）中进行蛋白质表达以及在大肠杆菌中在天然条件下进行蛋白质纯化。裂解液。介绍作为分子生物学实验室中非常有用的实践，重组DNA技术（或基因克隆）是指将DNA片段从一种生物体转移到自我复制的遗传元件如表达载体。然后插入的DNA可以在外源宿主细胞中繁殖
相关专题 1)噬菌体DE3溶源化的菌株如BL21(DE3)是最常用的表达菌株，噬菌体DE3是λ噬菌体的衍生株，构建好的表达载体 可以直接转入表达菌株中，诱导蛋白的表达方式与lac启动子一样都是IPTG诱导。 2)表达菌株BL21(DE3)上带有lacI基因，T7 RNA 聚合酶编码基因以及lacUV5启动子，lacUV5启动子可以启动T7 RNA 聚合酶的表达; 3)表达质粒 如pET-28质粒带有T7启动子和编码阻遏
Expression System、HisPatch ThioFusion System、pBAD Inducible Bacterial System、pL System和T7-based Systems。这四个系列的载体可谓把原核表达的发展史中出现过的几种启动子都囊括进去了，包括T7、pL、pBAD和trc启动子，同时几种经典的调控方式也都出现了，包括有乳糖操纵子、色氨酸操纵子和阿拉伯糖操纵子。 冠军系列 Champion™ pET Expression System之所以取
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pET-23c(+)载体
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