pBABE-neo-hTERT载体

pBABE-neo-hTERT载体

收藏
  • ¥1500
  • ZYbscience
  • 中国/美国
  • ZY1780
  • 2025年07月09日
    avatar
    上海泽叶生物科技有限公司
    10 年钻石会员

  • 企业认证

    点击 QQ 联系

    • 相关产品推荐更多 >

    万千商家帮你免费找货

    0 人在求购买到急需产品
    • 详细信息
    • 技术资料
    • 保存条件：

      -20℃低温保存

    • 保质期：

      三年

    • 英文名：

      pBABE-neo-hTERT

    • 库存：

      20

    • 供应商：

      泽叶生物

    载体基本信息

    出品人: ZYbscience
    载体名称: pBABE-neo-hTERT
    质粒类型: 逆病毒载体
    高拷贝/低拷贝: 高拷贝
    克隆方法: 限制性内切酶，多克隆位点
    启动子: MSCV
    载体大小: 8709 bp
    5' 测序引物及序列: pBABE 5' primer 5'CTTTATCCAGCCCTCAC3'
    3' 测序引物及序列: pBABE 3' primer 5'ACCCTAACTGACACACATTCC3'
    载体标签: 无
    载体抗性: 氨苄青霉素和卡那霉素
    筛选标记: Neomycin
    克隆菌株: DH5α、HB101等
    宿主细胞（系）: 常规哺乳动物细胞系
    备注:
    逆病毒载体pBABE-neo-hTERT表达telomerase reverse transcriptase，用来建永生细胞系。
    推荐使用逆病毒包装载体： 。
    产品目录号: #1780
    稳定性: 稳表达
    组成型/诱导型: 组成型
    病毒/非病毒: 逆转录病毒

    载体质粒图谱和多克隆位点信息

    pBABE-neo-hTERT载体图谱


    载体简介

    载体序列

    LOCUS       pBABE-neo-hTERT Sequencing Result	8709 bp 	DNA	SYN
DEFINITION  pBABE-neo-hTERT Sequencing Result
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..8709
                     /organism="pBABE-neo-hTERT Sequencing Result"
                     /mol_type="other DNA"
     misc_feature    10..477
                     /label="5_LTR2"
     misc_feature    16..477
                     /label="3MoMuLV_LTR"
     misc_feature    547..1356
                     /label="psi_plus_pack"
     misc_feature    943..1350
                     /label="gag"
     misc_feature    1286..1308
                     /label="MSCV_primer"
     misc_feature    1286..1308
                     /label="pLXSN_5_primer"
     misc_feature    1324..1340
                     /label="pBABE_5_primer"
     CDS             complement(1337..2050)
                     /label="ORF frame 3"
                     /translation="MVPGPFASQTPSGSTSVWRGPGLSGSAELVQRRPAHLVGAAGSH
                     QHKERAACQQVNQHVVAHAAQQQPPRPAPPQCVGHRVGQVAAHAGGEGLGGAPAGPVQ
                     QREAEGQHVLRAALAQPLQHSGHQLLQAGHLAEGGGGGGRPCVPGHAHQALGHQRAES
                     RRVPALHQPPALGPQAPHERGQRQHLAVVAAQQGAHGSAAGSARHRGGGRGQGFPRAQ
                     QDEFLRTTTLGSGRRLEKE*"
     CDS             1436..4834
                     /label="ORF frame 2"
                     /translation="MPRAPRCRAVRSLLRSHYREVLPLATFVRRLGPQGWRLVQRGDP
                     AAFRALVAQCLVCVPWDARPPPAAPSFRQVSCLKELVARVLQRLCERGAKNVLAFGFA
                     LLDGARGGPPEAFTTSVRSYLPNTVTDALRGSGAWGLLLRRVGDDVLVHLLARCALFV
                     LVAPSCAYQVCGPPLYQLGAATQARPPPHASGPRRRLGCERAWNHSVREAGVPLGLPA
                     PGARRRGGSASRSLPLPKRPRRGAAPEPERTPVGQGSWAHPGRTRGPSDRGFCVVSPA
                     RPAEEATSLEGALSGTRHSHPSVGRQHHAGPPSTSRPPRPWDTPCPPVYAETKHFLYS
                     SGDKEQLRPSFLLSSLRPSLTGARRLVETIFLGSRPWMPGTPRRLPRLPQRYWQMRPL
                     FLELLGNHAQCPYGVLLKTHCPLRAAVTPAAGVCAREKPQGSVAAPEEEDTDPRRLVQ
                     LLRQHSSPWQVYGFVRACLRRLVPPGLWGSRHNERRFLRNTKKFISLGKHAKLSLQEL
                     TWKMSVRGCAWLRRSPGVGCVPAAEHRLREEILAKFLHWLMSVYVVELLRSFFYVTET
                     TFQKNRLFFYRKSVWSKLQSIGIRQHLKRVQLRELSEAEVRQHREARPALLTSRLRFI
                     PKPDGLRPIVNMDYVVGARTFRREKRAERLTSRVKALFSVLNYERARRPGLLGASVLG
                     LDDIHRAWRTFVLRVRAQDPPPELYFVKVDVTGAYDTIPQDRLTEVIASIIKPQNTYC
                     VRRYAVVQKAAHGHVRKAFKSHVSTLTDLQPYMRQFVAHLQETSPLRDAVVIEQSSSL
                     NEASSGLFDVFLRFMCHHAVRIRGKSYVQCQGIPQGSILSTLLCSLCYGDMENKLFAG
                     IRRDGLLLRLVDDFLLVTPHLTHAKTFLRTLVRGVPEYGCVVNLRKTVVNFPVEDEAL
                     GGTAFVQMPAHGLFPWCGLLLDTRTLEVQSDYSSYARTSIRASLTFNRGFKAGRNMRR
                     KLFGVLRLKCHSLFLDLQVNSLQTVCTNIYKILLLQAYRFHACVLQLPFHQQVWKNPT
                     FFLRVISDTASLCYSILKAKNAGMSLGAKGAAGPLPSEAVQWLCHQAFLLKLTRHRVT
                     YVPLLGSLRTAQTQLSRKLPGTTLTALEAAANPALPSDFKTILD*"
     CDS             complement(2471..3691)
                     /label="ORF frame 3"
                     /translation="MGGLLDHGIPTHAVRVLGFDDAGDDLREPVLGDGVVRARHIHLD
                     KVQLRRRVLGPHTQHEGAPGPVDIVQAQHRGAQEAGAPRPLVVEHAEQCLHPRGETLG
                     PLFSAERSGSHDVVHVHNRPQPVRLGDEAESGRQQGGPGFPMLPDLCFRQLPQLHPLQ
                     VLSDSNALQLAPDTLPVEKEPVLLKRGLRDIKERPEQLDDVHTHQPVQELGQDLLTQT
                     VLCGRNTANPWAPAQPSAAPHAHLPRQLLQRELGMLPQGDELLGVPEEAAFVVPGAPE
                     AWGHQPAQAGPHEAVHLPGAAVLAEQLHQATGVCVLLLGGRHRALGLLPGTDTGCWGD
                     RSSQRAVRLEEHPVGALRVVPKQLQKQGPHLPVALGQAGQPAGSPWHPGPGTQKDGLH
                     EPPSASQAGPQRAE*"
     misc_feature    complement(4846..4866)
                     /label="pBABE_3_primer"
                     /translation="MGGLLDHGIPTHAVRVLGFDDAGDDLREPVLGDGVVRARHIHLD
                     KVQLRRRVLGPHTQHEGAPGPVDIVQAQHRGAQEAGAPRPLVVEHAEQCLHPRGETLG
                     PLFSAERSGSHDVVHVHNRPQPVRLGDEAESGRQQGGPGFPMLPDLCFRQLPQLHPLQ
                     VLSDSNALQLAPDTLPVEKEPVLLKRGLRDIKERPEQLDDVHTHQPVQELGQDLLTQT
                     VLCGRNTANPWAPAQPSAAPHAHLPRQLLQRELGMLPQGDELLGVPEEAAFVVPGAPE
                     AWGHQPAQAGPHEAVHLPGAAVLAEQLHQATGVCVLLLGGRHRALGLLPGTDTGCWGD
                     RSSQRAVRLEEHPVGALRVVPKQLQKQGPHLPVALGQAGQPAGSPWHPGPGTQKDGLH
                     EPPSASQAGPQRAE*"
     misc_feature    complement(4852..5067)
                     /label="SV40_enhancer"
                     /translation="MGGLLDHGIPTHAVRVLGFDDAGDDLREPVLGDGVVRARHIHLD
                     KVQLRRRVLGPHTQHEGAPGPVDIVQAQHRGAQEAGAPRPLVVEHAEQCLHPRGETLG
                     PLFSAERSGSHDVVHVHNRPQPVRLGDEAESGRQQGGPGFPMLPDLCFRQLPQLHPLQ
                     VLSDSNALQLAPDTLPVEKEPVLLKRGLRDIKERPEQLDDVHTHQPVQELGQDLLTQT
                     VLCGRNTANPWAPAQPSAAPHAHLPRQLLQRELGMLPQGDELLGVPEEAAFVVPGAPE
                     AWGHQPAQAGPHEAVHLPGAAVLAEQLHQATGVCVLLLGGRHRALGLLPGTDTGCWGD
                     RSSQRAVRLEEHPVGALRVVPKQLQKQGPHLPVALGQAGQPAGSPWHPGPGTQKDGLH
                     EPPSASQAGPQRAE*"
     promoter        4864..5132
                     /label="SV40_promoter"
                     /translation="MGGLLDHGIPTHAVRVLGFDDAGDDLREPVLGDGVVRARHIHLD
                     KVQLRRRVLGPHTQHEGAPGPVDIVQAQHRGAQEAGAPRPLVVEHAEQCLHPRGETLG
                     PLFSAERSGSHDVVHVHNRPQPVRLGDEAESGRQQGGPGFPMLPDLCFRQLPQLHPLQ
                     VLSDSNALQLAPDTLPVEKEPVLLKRGLRDIKERPEQLDDVHTHQPVQELGQDLLTQT
                     VLCGRNTANPWAPAQPSAAPHAHLPRQLLQRELGMLPQGDELLGVPEEAAFVVPGAPE
                     AWGHQPAQAGPHEAVHLPGAAVLAEQLHQATGVCVLLLGGRHRALGLLPGTDTGCWGD
                     RSSQRAVRLEEHPVGALRVVPKQLQKQGPHLPVALGQAGQPAGSPWHPGPGTQKDGLH
                     EPPSASQAGPQRAE*"
     rep_origin      5031..5108
                     /label="SV40_origin"
                     /translation="MGGLLDHGIPTHAVRVLGFDDAGDDLREPVLGDGVVRARHIHLD
                     KVQLRRRVLGPHTQHEGAPGPVDIVQAQHRGAQEAGAPRPLVVEHAEQCLHPRGETLG
                     PLFSAERSGSHDVVHVHNRPQPVRLGDEAESGRQQGGPGFPMLPDLCFRQLPQLHPLQ
                     VLSDSNALQLAPDTLPVEKEPVLLKRGLRDIKERPEQLDDVHTHQPVQELGQDLLTQT
                     VLCGRNTANPWAPAQPSAAPHAHLPRQLLQRELGMLPQGDELLGVPEEAAFVVPGAPE
                     AWGHQPAQAGPHEAVHLPGAAVLAEQLHQATGVCVLLLGGRHRALGLLPGTDTGCWGD
                     RSSQRAVRLEEHPVGALRVVPKQLQKQGPHLPVALGQAGQPAGSPWHPGPGTQKDGLH
                     EPPSASQAGPQRAE*"
     misc_feature    5093..5112
                     /label="SV40pro_F_primer"
                     /translation="MGGLLDHGIPTHAVRVLGFDDAGDDLREPVLGDGVVRARHIHLD
                     KVQLRRRVLGPHTQHEGAPGPVDIVQAQHRGAQEAGAPRPLVVEHAEQCLHPRGETLG
                     PLFSAERSGSHDVVHVHNRPQPVRLGDEAESGRQQGGPGFPMLPDLCFRQLPQLHPLQ
                     VLSDSNALQLAPDTLPVEKEPVLLKRGLRDIKERPEQLDDVHTHQPVQELGQDLLTQT
                     VLCGRNTANPWAPAQPSAAPHAHLPRQLLQRELGMLPQGDELLGVPEEAAFVVPGAPE
                     AWGHQPAQAGPHEAVHLPGAAVLAEQLHQATGVCVLLLGGRHRALGLLPGTDTGCWGD
                     RSSQRAVRLEEHPVGALRVVPKQLQKQGPHLPVALGQAGQPAGSPWHPGPGTQKDGLH
                     EPPSASQAGPQRAE*"
     promoter        5399..5448
                     /label="NEOKAN_promoter"
                     /translation="MGGLLDHGIPTHAVRVLGFDDAGDDLREPVLGDGVVRARHIHLD
                     KVQLRRRVLGPHTQHEGAPGPVDIVQAQHRGAQEAGAPRPLVVEHAEQCLHPRGETLG
                     PLFSAERSGSHDVVHVHNRPQPVRLGDEAESGRQQGGPGFPMLPDLCFRQLPQLHPLQ
                     VLSDSNALQLAPDTLPVEKEPVLLKRGLRDIKERPEQLDDVHTHQPVQELGQDLLTQT
                     VLCGRNTANPWAPAQPSAAPHAHLPRQLLQRELGMLPQGDELLGVPEEAAFVVPGAPE
                     AWGHQPAQAGPHEAVHLPGAAVLAEQLHQATGVCVLLLGGRHRALGLLPGTDTGCWGD
                     RSSQRAVRLEEHPVGALRVVPKQLQKQGPHLPVALGQAGQPAGSPWHPGPGTQKDGLH
                     EPPSASQAGPQRAE*"
     CDS             5537..6331
                     /label="ORF frame 2"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     gene            5540..6328
                     /label="NeoR/KanR"
                     /gene="NeoR/KanR"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
     CDS             complement(5846..6352)
                     /label="ORF frame 3"
                     /translation="MRTPESRSEELVKKAIEGDALRIGSGDTVKHEEAVSPFAAKLFS
                     NITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILAVGHARLEPGE
                     QFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQPPHCISHDGY
                     FLGRSKVR*"
     gene            complement(7719..8579)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MRTPESRSEELVKKAIEGDALRIGSGDTVKHEEAVSPFAAKLFS
                     NITGSQRYVLIAVRHTQPATVDESRKAAIFHHDIRQAGIAMGHDEILAVGHARLEPGE
                     QFGWREPLMLFVQIILIDKTGFHPSTCSLDAMFRLVVEWAGSRIKRMQPPHCISHDGY
                     FLGRSKVR*"
     CDS             complement(7719..8579)
                     /label="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(8621..8649)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"

    风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。

    图标技术资料

    暂无技术资料 索取技术资料

    同类产品报价

    产品名称
    产品价格
    公司名称
    报价日期
    pBABE-hygro-hTERT载体
    ¥1500
    上海泽叶生物科技有限公司
    2025年07月08日询价
    载体pBABE-neo largeT cDNA
    ¥800
    上海沪震实业有限公司
    2025年07月14日询价
    pBABE-Puro载体
    ¥1000
    上海信裕生物科技有限公司
    2025年07月13日询价
    pBABE-Puro载体
    ￥1000
    上海盖宁生物科技有限公司
    2025年11月18日询价
    pBABE-Puro载体
    ¥1000
    上海再康生物科技有限公司
    2025年11月20日询价
    pBABE-neo-hTERT载体
    ¥1500