| 出品公司: | ZYbscience |
|---|---|
| 载体名称: | pHANNIBAL |
| 质粒类型: | 植物RNAi载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | CaMV35S |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5824 bp |
| 5' 测序引物及序列: | 35S promoter: CTATCCTTCGCAAGACCCTTC |
| 3' 测序引物及序列: | -- |
| NCBI序列编号: | AJ311872 |
| 载体抗性: | 氨苄 |
| 筛选标记: | -- |
| 备注: | 参考文献：
Authors: Wesley,S.V., Helliwell,C.A., Smith,N.A., Wang,M.B., Rouse,D.T., Liu,Q., Gooding,P.S., Singh,S.P., Abbott,D., Stoutjesdijk,P.A., Robinson,S.P., Gleave,A.P., Green,A.G. and Waterhouse,P.M.
Title: Construct design for efficient, effective and high-throughput gene silencing in plants
Journal: Plant J. 27 (6), 581-590 (2001)
|
| 产品目录号: | -- |
| 稳定性: | 稳定 |
| 组成型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃低温保存
- 保质期：
三年
- 英文名：
pHANNIBAL
- 库存：
20
- 供应商：
泽叶生物
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pHannibal 5824 bp ds-DNA circular SYN 18-5月-2013
DEFINITION Vector for silencing genes in plants using intron-containing hairpin
RNA (hpRNA).
ACCESSION AJ311872
VERSION .
KEYWORDS pHANNIBAL
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5824)
AUTHORS Wesley SV, Helliwell CA, Smith NA, Wang MB, Rouse DT, Liu Q, Gooding
PS, Singh SP, Abbott D, Stoutjesdijk PA, Robinson SP, Gleave AP,
Green AG, Waterhouse PM.
TITLE Construct design for efficient, effective and high-throughput gene
silencing in plants.
JOURNAL Plant J. 2001;27:581-90.
PUBMED 11576441
REFERENCE 2 (bases 1 to 5824)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported 2016年3月7日 from SnapGene 1.1.3
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5824
/organism="synthetic DNA construct"
/lab_host="Plant Cells"
/mol_type="other DNA"
rep_origin complement(215..643)
/direction=LEFT
/note="f1 ori"
/note="color: #ffff00"
promoter 670..774
/note="AmpR promoter"
/note="color: #ffffff; direction: RIGHT"
CDS 775..1635
/codon_start=1
/note="AmpR"
/note="This feature has 2 segments:
???1:??775?..??843?/?#ccffcc?/?signal?sequence
???2:??844?..?1635?/?#ccffcc
Cleavage site after base 843"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 1806..2394
/direction=RIGHT
/note="ori"
/note="color: #ffff00"
promoter 3863..4208
/note="CaMV 35S promoter"
/note="color: #ffffff; direction: RIGHT"
misc_feature 4210..4228
/note="MCS 1"
/note="color: #99ccff"
intron 4236..5002
/note="PDK intron"
/note="color: #------"
misc_feature 5022..5045
/note="MCS 2"
/note="color: #99ccff"
terminator 5049..5755
/note="OCS terminator"
/note="color: #ffffff"
ORIGIN
1 tgaccaagtc agcttggcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
61 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
121 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggaaat
181 tgtaaacgtt aatattttgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
241 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
301 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
361 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
421 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
481 gggatgcccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
541 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
601 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg
661 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
721 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
781 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
841 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
901 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
961 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
1021 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
1081 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
1141 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
1201 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
1261 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
1321 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
1381 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
1441 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
1501 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
1561 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
1621 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
1681 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
1741 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
1801 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
1861 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
1921 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
1981 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
2041 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
2101 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
2161 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
2221 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
2281 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
2341 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
2401 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
2461 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
2521 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
2581 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
2641 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
2701 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
2761 agcggataac aatttcacac aggaaacagc tatgaccatg attacgaatt tggccaagtc
2821 ggcctctaat acgactcact atagggagct cgtcgagcgg ccgctcgacg aattaattcc
2881 aatcccacaa aaatctgagc ttaacagcac agttgctcct ctcagagcag aatcgggtat
2941 tcaacaccct catatcaact actacgttgt gtataacggt ccacatgccg gtatatacga
3001 tgactggggt tgtacaaagg cggcaacaaa cggcgttccc ggagttgcac acaagaaatt
3061 tgccactatt acagaggcaa gagcagcagc tgacgcgtac acaacaagtc agcaaacaga
3121 caggttgaac ttcatcccca aaggagaagc tcaactcaag cccaagagct ttgctaaggc
3181 cctaacaagc ccaccaaagc aaaaagccca ctggctcacg ctaggaacca aaaggcccag
3241 cagtgatcca gccccaaaag agatctcctt tgccccggag attacaatgg acgatttcct
3301 ctatctttac gatctaggaa ggaagttcga aggtgaaggt gacgacacta tgttcaccac
3361 tgataatgag aaggttagcc tcttcaattt cagaaagaat gctgacccac agatggttag
3421 agaggcctac gcagcaggtc tcatcaagac gatctacccg agtaacaatc tccaggagat
3481 caaatacctt cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta attgcatcaa
3541 gaacacagag aaagacatat ttctcaagat cagaagtact attccagtat ggacgattca
3601 aggcttgctt cataaaccaa ggcaagtaat agagattgga gtctctaaaa aggtagttcc
3661 tactgaatct aaggccatgc atggagtcta agattcaaat cgaggatcta acagaactcg
3721 ccgtgaagac tggcgaacag ttcatacaga gtcttttacg actcaatgac aagaagaaaa
3781 tcttcgtcaa catggtggag cacgacactc tggtctactc caaaaatgtc aaagatacag
3841 tctcagaaga ccaaagggct attgagactt ttcaacaaag gataatttcg ggaaacctcc
3901 tcggattcca ttgcccagct atctgtcact tcatcgaaag gacagtagaa aaggaaggtg
3961 gctcctacaa atgccatcat tgcgataaag gaaaggctat cattcaagat ctctctgccg
4021 acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc
4081 caaccacgtc ttcaaagcaa gtggattgat gtgacatctc cactgacgta agggatgacg
4141 cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg
4201 agaggacacg ctcgaggaat tcggtacccc aattggtaag gaaataatta ttttcttttt
4261 tccttttagt ataaaatagt taagtgatgt taattagtat gattataata atatagttgt
4321 tataattgtg aaaaaataat ttataaatat attgtttaca taaacaacat agtaatgtaa
4381 aaaaatatga caagtgatgt gtaagacgaa gaagataaaa gttgagagta agtatattat
4441 ttttaatgaa tttgatcgaa catgtaagat gatatactag cattaatatt tgttttaatc
4501 ataatagtaa ttctagctgg tttgatgaat taaatatcaa tgataaaata ctatagtaaa
4561 aataagaata aataaattaa aataatattt ttttatgatt aatagtttat tatataatta
4621 aatatctata ccattactaa atattttagt ttaaaagtta ataaatattt tgttagaaat
4681 tccaatctgc ttgtaattta tcaataaaca aaatattaaa taacaagcta aagtaacaaa
4741 taatatcaaa ctaatagaaa cagtaatcta atgtaacaaa acataatcta atgctaatat
4801 aacaaagcgc aagatctatc attttatata gtattatttt caatcaacat tcttattaat
4861 ttctaaataa tacttgtagt tttattaact tctaaatgga ttgactatta attaaatgaa
4921 ttagtcgaac atgaataaac aaggtaacat gatagatcat gtcattgtgt tatcattgat
4981 cttacatttg gattgattac agttgggaaa ttgggttcga aatcgataag cttggatcct
5041 ctagagtcct gctttaatga gatatgcgag acgcctatga tcgcatgata tttgctttca
5101 attctgttgt gcacgttgta aaaaacctga gcatgtgtag ctcagatcct taccgccggt
5161 ttcggttcat tctaatgaat atatcacccg ttactatcgt atttttatga ataatattct
5221 ccgttcaatt tactgattgt accctactac ttatatgtac aatattaaaa tgaaaacaat
5281 atattgtgct gaataggttt atagcgacat ctatgataga gcgccacaat aacaaacaat
5341 tgcgttttat tattacaaat ccaattttaa aaaaagcggc agaaccggtc aaacctaaaa
5401 gactgattac ataaatctta ttcaaatttc aaaaggcccc aggggctagt atctacgaca
5461 caccgagcgg cgaactaata acgttcactg aagggaactc cggttccccg ccggcgcgca
5521 tgggtgagat tccttgaagt tgagtattgg ccgtccgctc taccgaaagt tacgggcacc
5581 attcaacccg gtccagcacg gcggccgggt aaccgacttg ctgccccgag aattatgcag
5641 catttttttg gtgtatgtgg gccccaaatg aagtgcaggt caaaccttga cagtgacgac
5701 aaatcgttgg gcgggtccag ggcgaatttt gcgacaacat gtcgaggctc agcaggacct
5761 gcaggcatgc aagctagctt actagtgatg catattctat agtgtcacct aaatctgcgg
5821 ccgc
//
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pHANNIBAL载体
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