pLVX-DD-AmCyan1 Reporte载体

pLVX-DD-AmCyan1 Reporter is a promoterless reporter vector that allows the functional analysis of different promoters
and promoter/enhancer combinations inserted into its multiple cloning site (MCS). The vector encodes the reporter protein
DD-AmCyan1, a ligand-dependent, destabilized cyan fluorescent protein that minimizes background fluorescence from
leaky promoters. A promoter must be cloned into the MCS, located upstream of the DD-AmCyan1 coding sequence.
Without the addition of a functional promoter, the vector will not express DD-AmCyan1.

Location of Features
5' LTR: 1–635
PBS (primer binding site): 636–653
Ψ (packaging signal): 685–822
RRE (Rev-response element): 1303–1536
cPPT/CTS (central polypurine tract/central termination sequence): 2028–2151
MCS (multiple cloning site): 2195–2234
DD (FKBP-L106P destabilization domain): 2247–2570
AmCyan1 (Anemonia majano cyan fluorescent protein): 2577–3263
PPGK (phosphoglycerate kinase promoter): 3274–3782
Puror(puromycin resistance gene): 3803–4402
WPRE (woodchuck hepatitis virus posttranscriptional regulatory element): 4416–5007
3' LTR: 5211–5847
pUC origin of replication: 6317–6987 (complementary)
Ampr(ampicillin resistance gene; β-lactamase): 7132–8128 (complementary)