pLOX-CW-CRE载体

pLOX-CW-CRE载体

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  • ¥1000
  • ZYbscience
  • 中国/美国
  • ZY 12238
  • 2025年07月13日
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    上海泽叶生物科技有限公司
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    • 详细信息
    • 技术资料
    • 保存条件：

      -20℃低温保存

    • 保质期：

      三年

    • 英文名：

      pLOX-CW-CRE

    • 库存：

      20

    • 供应商：

      泽叶生物

    载体基本信息

    出品公司: ZYbscience
    载体名称: pLOX-CW-CRE
    质粒类型: 慢病毒系统载体
    高拷贝/低拷贝: 高拷贝
    启动子: CMV
    克隆方法: 多克隆位点，限制性内切酶
    载体大小: 9886bp
    5' 测序引物及序列: CMV forward : CGCAAATGGGCGGTAGGCGTG
    3' 测序引物及序列: SP6 : ATTTAGGTGACACTATAG
    载体标签: --
    载体抗性: 氨苄青霉素（Ampicillian）
    筛选标记: --
    备注: 克隆菌株为 Stbl3或HB101。
    产品目录号: ZY12238
    稳定性: --
    组成型: --
    病毒/非病毒: 慢病毒

    载体质粒图谱和多克隆位点信息

    pLOX-CW-CRE载体图谱


    载体简介

    载体序列

    LOCUS       pLOX-CW-CRE	9886 bp 	DNA	SYN
DEFINITION  pLOX-CW-CRE
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..9886
                     /organism="pLOX-CW-CRE"
                     /mol_type="other DNA"
     misc_feature    457..637
                     /label="HIV-1_5_LTR"
     misc_feature    457..637
                     /label="truncHIV-1_3_LTR"
     misc_feature    748..792
                     /label="HIV-1_psi_pack"
     CDS             1180..2028
                     /label="ORF frame 1"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYR*"
     misc_feature    1302..1535
                     /label="RRE"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYR*"
     promoter        2040..2544
                     /label="CMV_immearly_promoter"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYR*"
     misc_feature    2047..2334
                     /label="CAG_enhancer"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYR*"
     misc_feature    2501..2521
                     /label="CMV_fwd_primer"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYR*"
     promoter        2502..2571
                     /label="CMV_promoter"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYR*"
     misc_feature    2545..2564
                     /label="pCEP_fwd_primer"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYR*"
     misc_feature    2547..2571
                     /label="LNCX_primer"
                     /translation="MRDNWRSELYKYKVVKIEPLGVAPTKAKRRVVQREKRAVGIGAL
                     FLGFLGAAGSTMGAASMTLTVQARQLLSGIVQQQNNLLRAIEAQQHLLQLTVWGIKQL
                     QARILAVERYLKDQQLLGIWGCSGKLICTTAVPWNASWSNKSLEQIWNHTTWMEWDRE
                     INNYTSLIHSLIEESQNQQEKNEQELLELDKWASLWNWFNITNWLWYIKLFIMIVGGL
                     VGLRIVFAVLSIVNRVRQGYSPLSFQTHLPTPRGPDRPEGIEEEGGERDRDRSIRLVN
                     GSRRYR*"
     CDS             2640..3692
                     /label="ORF frame 3"
                     /translation="MPKKKRKVSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAF
                     SEHTWKMLLSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQL
                     NMLHRRSGLPRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSD
                     RCQDIRNLAFLGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEK
                     ALSLGVTKLVERWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATH
                     RLIYGAKDDSGQRYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRN
                     LDSETGAMVRLLEDGD*"
     misc_feature    2643..2663
                     /label="SV40 NLS"
                     /translation="MPKKKRKVSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAF
                     SEHTWKMLLSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQL
                     NMLHRRSGLPRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSD
                     RCQDIRNLAFLGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEK
                     ALSLGVTKLVERWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATH
                     RLIYGAKDDSGQRYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRN
                     LDSETGAMVRLLEDGD*"
     misc_feature    2649..3692
                     /label="CRE_NLS"
                     /translation="MPKKKRKVSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAF
                     SEHTWKMLLSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQL
                     NMLHRRSGLPRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSD
                     RCQDIRNLAFLGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEK
                     ALSLGVTKLVERWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATH
                     RLIYGAKDDSGQRYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRN
                     LDSETGAMVRLLEDGD*"
     misc_feature    2649..2663
                     /label="NLS"
                     /translation="MPKKKRKVSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAF
                     SEHTWKMLLSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQL
                     NMLHRRSGLPRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSD
                     RCQDIRNLAFLGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEK
                     ALSLGVTKLVERWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATH
                     RLIYGAKDDSGQRYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRN
                     LDSETGAMVRLLEDGD*"
     CDS             3682..4368
                     /label="ORF frame 1"
                     /translation="MAIRSSSSFEFDNQPLDYKICERLTGILNYVAPFTLCGYAALMP
                     LYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPTGWGIATTCQL
                     LSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGKLTSFPWLLACVATWIL
                     RGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASPHREFELGTFK
                     TNDLQGSCRS*"
     misc_feature    3720..4312
                     /label="WPRE"
                     /translation="MAIRSSSSFEFDNQPLDYKICERLTGILNYVAPFTLCGYAALMP
                     LYHAIASRMAFIFSSLYKSWLLSLYEELWPVVRQRGVVCTVFADATPTGWGIATTCQL
                     LSGTFAFPLPIATAELIAACLARCWTGARLLGTDNSVVLSGKLTSFPWLLACVATWIL
                     RGTSFCYVPSALNPADLPSRGLLPALRPLPRLRLRPQTSRISLWAASPHREFELGTFK
                     TNDLQGSCRS*"
     CDS             complement(3805..4308)
                     /label="ORF frame 2"
                     /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA
                     EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES
                     ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS
                     NSMIQRH*"
     misc_feature    4378..4399
                     /label="U3PPT"
                     /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA
                     EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES
                     ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS
                     NSMIQRH*"
     misc_feature    4378..4393
                     /label="cPPT"
                     /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA
                     EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES
                     ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS
                     NSMIQRH*"
     misc_feature    4450..4483
                     /label="loxP"
                     /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA
                     EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES
                     ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS
                     NSMIQRH*"
     misc_feature    4503..4683
                     /label="HIV-1_5_LTR"
                     /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA
                     EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES
                     ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS
                     NSMIQRH*"
     misc_feature    4503..4683
                     /label="truncHIV-1_3_LTR"
                     /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA
                     EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES
                     ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS
                     NSMIQRH*"
     misc_feature    complement(4709..4726)
                     /label="Sp6_primer"
                     /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA
                     EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES
                     ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS
                     NSMIQRH*"
     gene            4964..5227
                     /label="tet (300 - 563)"
                     /gene="tet (300 - 563)"
                     /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA
                     EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES
                     ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS
                     NSMIQRH*"
     misc_feature    complement(5016..5035)
                     /label="pBRrevBam_primer"
                     /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA
                     EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES
                     ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS
                     NSMIQRH*"
     misc_feature    5368..5390
                     /label="pGEX_3_primer"
                     /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA
                     EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES
                     ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS
                     NSMIQRH*"
     promoter        5549..5577
                     /label="AmpR_promoter"
                     /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA
                     EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES
                     ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS
                     NSMIQRH*"
     gene            5619..6479
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MRGGGPKGDPTRLRAKAKTRKRPQSRQQAAGRKVRWIEGRRDVA
                     EGRPAQNPGGNTGEQPWKGRQLPRQHHGIVSAQQPSPCPAAGKAGGDEFRRGNREGES
                     ESPGKELTGGGNAPTSGGCVSKHSAHHATLPDNGPQLLIKRQQPGFIQGGENESHTGS
                     NSMIQRH*"
     CDS             5619..6479
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      6634..7253
                     /label="pBR322_origin"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    complement(7479..7499)
                     /label="pBABE_3_primer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     misc_feature    complement(7485..7938)
                     /label="SV40_enhancer"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        7497..7765
                     /label="SV40_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     rep_origin      7664..7741

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