| 出品公司: | ZYbscience |
|---|---|
| 载体名称: | pShuttle-CMV-EGFP-C |
| 质粒类型: | 腺病毒载体 |
| 高拷贝/低拷贝: | 低拷贝 |
| 启动子: | CMV |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 8156 bp |
| 5' 测序引物及序列: | GGCACCAAAATCAACGGGACTTTCC |
| 3' 测序引物及序列: | -- |
| 载体标签: | EGFP |
| 载体抗性: | Kanamycin |
| 筛选标记: | -- |
| 备注: | 克隆菌株：DH5alpha |
| 产品目录号: | ZY13887 |
| 稳定性: | -- |
| 组成型: | -- |
| 病毒/非病毒: | 腺病毒 |
- ¥1600
- ZYbscience
- 中国/美国
- ZY
- 2025年07月16日
11 年钻石会员
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃低温保存
- 保质期：
三年
- 英文名：
pShuttle-CMV-EGFP-C
- 库存：
20
- 供应商：
泽叶生物
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pShuttle-CMV-EGFP-C 8156 bp DNA SYN
DEFINITION pShuttle-CMV-EGFP-C
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..8156
/organism="pShuttle-CMV-EGFP-C"
/mol_type="other DNA"
misc_feature complement(27..105)
/label="RITR"
misc_feature 27..105
/label="LITR"
misc_feature 185..333
/label="Encap"
promoter 349..901
/label="CMV_immearly_promoter"
misc_feature 404..691
/label="CAG_enhancer"
misc_feature 858..878
/label="CMV_fwd_primer"
promoter 859..928
/label="CMV_promoter"
CDS 972..1763
/label="ORF frame 3"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
MVLLEFVTAAGITLGMDELYKSGLRSVGALMEVMQKRSKAIHSSD*"
gene 975..1688
/label="EGFP"
/gene="EGFP"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
MVLLEFVTAAGITLGMDELYKSGLRSVGALMEVMQKRSKAIHSSD*"
misc_feature complement(1017..1038)
/label="EGFP_N_primer"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
MVLLEFVTAAGITLGMDELYKSGLRSVGALMEVMQKRSKAIHSSD*"
misc_feature 1155..1184
/label="Y66 (EGFP)"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
MVLLEFVTAAGITLGMDELYKSGLRSVGALMEVMQKRSKAIHSSD*"
misc_feature 1625..1646
/label="EGFP_C_primer"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
MVLLEFVTAAGITLGMDELYKSGLRSVGALMEVMQKRSKAIHSSD*"
terminator 1805..2033
/label="SV40_PA_terminator"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
MVLLEFVTAAGITLGMDELYKSGLRSVGALMEVMQKRSKAIHSSD*"
misc_feature 2003..2022
/label="EBV_rev_primer"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLT
LKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK
DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
GIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDH
MVLLEFVTAAGITLGMDELYKSGLRSVGALMEVMQKRSKAIHSSD*"
CDS complement(2609..3967)
/label="ORF frame 2"
/translation="MLPGRRAGRRPAALQHQQDQPQAHPGQRAARSAPLHRDPDYADE
DPAPVERHDPGPSGRAPTTAVQRKPPQPAKRGDMLDRDAVEQVTELWDRLELLGQTLK
SMPTADGLKPLKNFASLQELLSLGGERLLADLVRENMRVRDMLNEVAPLLRDDGSCSS
LNYQLHPVIGVIYGPTGCGKSQLLRNLLSSQLISPTPETVFFIAPQVDMIPPSELKAW
EMQICEGNYAPGPDGTIIPQSGTLRPRFVKMAYDDLILEHNYDVSDPRNIFAQAAARG
PIAIIMDECMENLGGHKGVSKFFHAFPSKLHDKFPKCTGYTVLVVLHNMNPRRDMAGN
IANLKIQSKMHLISPRMHPSQLNRFVNTYTKGLPLAISLLLKDIFRHHAQRSCYDWII
YNTTPQHEALQWCYLHPRDGLMPMYLNIQSHLYHVLEKIHRTLNDRDRWSRAYRARKT
PK*"
CDS complement(3715..4236)
/label="ORF frame 3"
/translation="METRGKKRIKKHGGNLVFDPERPELTWLVECETVCGACGADAYS
PESVFLAPKLYALKSLHCPSCGASSKGKLRAKGHAAEGLDYDTMVKCYLADAQGEDRQ
RFSTSRTSLKRTLASAQPGAHPFTVTQTTLTRTLRPWKDMTLARLDEHRLLPYSESRP
NPRNEEICWIEMP*"
misc_feature complement(5194..5289)
/label="LITR"
/translation="METRGKKRIKKHGGNLVFDPERPELTWLVECETVCGACGADAYS
PESVFLAPKLYALKSLHCPSCGASSKGKLRAKGHAAEGLDYDTMVKCYLADAQGEDRQ
RFSTSRTSLKRTLASAQPGAHPFTVTQTTLTRTLRPWKDMTLARLDEHRLLPYSESRP
NPRNEEICWIEMP*"
misc_feature 5194..5289
/label="RITR"
/translation="METRGKKRIKKHGGNLVFDPERPELTWLVECETVCGACGADAYS
PESVFLAPKLYALKSLHCPSCGASSKGKLRAKGHAAEGLDYDTMVKCYLADAQGEDRQ
RFSTSRTSLKRTLASAQPGAHPFTVTQTTLTRTLRPWKDMTLARLDEHRLLPYSESRP
NPRNEEICWIEMP*"
rep_origin complement(5534..6153)
/label="pBR322_origin"
/translation="METRGKKRIKKHGGNLVFDPERPELTWLVECETVCGACGADAYS
PESVFLAPKLYALKSLHCPSCGASSKGKLRAKGHAAEGLDYDTMVKCYLADAQGEDRQ
RFSTSRTSLKRTLASAQPGAHPFTVTQTTLTRTLRPWKDMTLARLDEHRLLPYSESRP
NPRNEEICWIEMP*"
gene complement(6308..6607)
/label="Ampicillin (299 - 0)"
/gene="Ampicillin (299 - 0)"
/translation="METRGKKRIKKHGGNLVFDPERPELTWLVECETVCGACGADAYS
PESVFLAPKLYALKSLHCPSCGASSKGKLRAKGHAAEGLDYDTMVKCYLADAQGEDRQ
RFSTSRTSLKRTLASAQPGAHPFTVTQTTLTRTLRPWKDMTLARLDEHRLLPYSESRP
NPRNEEICWIEMP*"
promoter 6810..6859
/label="NEOKAN_promoter"
/translation="METRGKKRIKKHGGNLVFDPERPELTWLVECETVCGACGADAYS
PESVFLAPKLYALKSLHCPSCGASSKGKLRAKGHAAEGLDYDTMVKCYLADAQGEDRQ
RFSTSRTSLKRTLASAQPGAHPFTVTQTTLTRTLRPWKDMTLARLDEHRLLPYSESRP
NPRNEEICWIEMP*"
CDS 6948..7742
/label="ORF frame 3"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
gene 6951..7739
/label="NeoR/KanR"
/gene="NeoR/KanR"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"
ORIGIN
1 NNTCCCTTCC AGCTCTCTGC CCCTTTTGGA TTGAAGCCAA TATGATAATG AGGGGGTGGA
61 GTTTGTGACG TGGCGCGGGG CGTGGGAACG GGGCGGGTGA CGTAGTAGTG TGGCGGAAGT
121 GTGATGTTGC AAGTGTGGCG GAACACATGT AAGCGACGGA TGTGGCAAAA GTGACGTTTT
181 TGGTGTGCGC CGGTGTACAC AGGAAGTGAC AATTTTCGCG CGGTTTTAGG CGGATGTTGT
241 AGTAAATTTG GGCGTAACCG AGTAAGATTT GGCCATTTTC GCGGGAAAAC TGAATAAGAG
301 GAAGTGAAAT CTGAATAATT TTGTGTTACT CATAGCGCGT AANNNNTAAT AGTAATCAAT
361 TACGGGGTCA TTAGTTCATA GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA
421 TGGCCCGCCT GGCTGACCGC CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT
481 TCCCATAGTA ACGCCAATAG GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA
541 AACTGCCCAC TTGGCAGTAC ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT
601 CAATGACGGT AAATGGCCCG CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC
661 TACTTGGCAG TACATCTACG TATTAGTCAT CGCTATTACC ATGGTGATGC GGTTTTGGCA
721 GTACATCAAT GGGCGTGGAT AGCGGTTTGA CTCACGGGGA TTTCCAAGTC TCCACCCCAT
781 TGACGTCAAT GGGAGTTTGT TTTGGCACCA AAATCAACGG GACTTTCCAA AATGTCGTAA
841 CAACTCCGCC CCATTGACGC AAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
901 CAGAGCTGGT TTAGTGAACC GTCAGATCCG CTAGAGATCg atcTGGTACC GTCGACGTCG
961 CGGCCGCCAC CATGGTGAGC AAGGGCGAGG AGCTGTTCAC CGGGGTGGTG CCCATCCTGG
1021 TCGAGCTGGA CGGCGACGTA AACGGCCACA AGTTCAGCGT GTCCGGCGAG GGCGAGGGCG
1081 ATGCCACCTA CGGCAAGCTG ACCCTGAAGT TCATCTGCAC CACCGGCAAG CTGCCCGTGC
1141 CCTGGCCCAC CCTCGTGACC ACCCTGACCT ACGGCGTGCA GTGCTTCAGC CGCTACCCCG
1201 ACCACATGAA GCAGCACGAC TTCTTCAAGT CCGCCATGCC CGAAGGCTAC GTCCAGGAGC
1261 GCACCATCTT CTTCAAGGAC GACGGCAACT ACAAGACCCG CGCCGAGGTG AAGTTCGAGG
1321 GCGACACCCT GGTGAACCGC ATCGAGCTGA AGGGCATCGA CTTCAAGGAG GACGGCAACA
1381 TCCTGGGGCA CAAGCTGGAG TACAACTACA ACAGCCACAA CGTCTATATC ATGGCCGACA
1441 AGCAGAAGAA CGGCATCAAG GTGAACTTCA AGATCCGCCA CAACATCGAG GACGGCAGCG
1501 TGCAGCTCGC CGACCACTAC CAGCAGAACA CCCCCATCGG CGACGGCCCC GTGCTGCTGC
1561 CCGACAACCA CTACCTGAGC ACCCAGTCCG CCCTGAGCAA AGACCCCAAC GAGAAGCGCG
1621 ATCACATGGT CCTGCTGGAG TTCGTGACCG CCGCCGGGAT CACTCTCGGC ATGGACGAGC
1681 TGTACAAGTC CGGACTCAGA TCTGTAGGTG CATTAATGGA AGTGATGCAG AAGAGGAGCA
1741 AAGCCATTCA TTCTTCAGAC TGAAGCTTCT AGATAAGATA TCCGATCCAC CGGATCTAGA
1801 TAACTGATCA TAATCAGCCA TACCACATTT GTAGAGGTTT TACTTGCTTT AAAAAACCTC
1861 CCACACCTCC CCCTGAACCT GAAACATAAA ATGAATGCAA TTGTTGTTGT TAACTTGTTT
1921 ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA
1981 TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTAACGCGGA
2041 Tctgggcgtg gtTAAGGGTG GGAAAGAATA TATAAGGTGG GGGTCTTATG TAGTTTTGTA
2101 TCTGTTTTGC AGCAGCCGCC GCCGCCATGA GCACCAACTC GTTTGATGGA AGCATTGTGA
2161 GCTCATATTT GACAACGCGC ATGCCCCCAT GGGCCGGGGT GCGTCAGAAT GTGATGGGCT
2221 CCAGCATTGA TGGTCGCCCC GTCCTGCCCG CAAACTCTAC TACCTTGACC TACGAGACCG
2281 TGTCTGGAAC GCCGTTGGAG ACTGCAGCCT CCGCCGCCGC TTCAGCCGCT GCAGCCACCG
2341 CCCGCGGGAT TGTGACTGAC TTTGCTTTCC TGAGCCCGCT TGCAAGCAGT GCAGCTTCCC
2401 GTTCATCCGC CCGCGATGAC AAGTTGACGG CTCTTTTGGC ACAATTGGAT TCTTTGACCC
2461 GGGAACTTAA TGTCGTTTCT CAGCAGCTGT TGGATCTGCG CCAGCAGGTT TCTGCCCTGA
2521 AGGCTTCCTC CCCTCCCAAT GCGGTTTAAA ACATAAATAA AAAACCAGAC TCTGTTTGGA
2581 TTTGGATCAA GCAAGTGTCT TGCTGTCTTT ATTTAGGGGT TTTGCGCGCG CGGTAGGCCC
2641 GGGACCAGCG GTCTCGGTCG TTGAGGGTCC TGTGTATTTT TTCCAGGACG TGGTAAAGGT
2701 GACTCTGGAT GTTCAGATAC ATGGGCATAA GCCCGTCTCT GGGGTGGAGG TAGCACCACT
2761 GCAGAGCTTC ATGCTGCGGG GTGGTGTTGT AGATGATCCA GTCGTAGCAG GAGCGCTGGG
2821 CGTGGTGCCT AAAAATGTCT TTCAGTAGCA AGCTGATTGC CAGGGGCAGG CCCTTGGTGT
2881 AAGTGTTTAC AAAGCGGTTA AGCTGGGATG GGTGCATACG TGGGGATATG AGATGCATCT
2941 TGGACTGTAT TTTTAGGTTG GCTATGTTCC CAGCCATATC CCTCCGGGGA TTCATGTTGT
3001 GCAGAACCAC CAGCACAGTG TATCCGGTGC ACTTGGGAAA TTTGTCATGT AGCTTAGAAG
3061 GAAATGCGTG GAAGAACTTG GAGACGCCCT TGTGACCTCC AAGATTTTCC ATGCATTCGT
3121 CCATAATGAT GGCAATGGGC CCACGGGCGG CGGCCTGGGC GAAGATATTT CTGGGATCAC
3181 TAACGTCATA GTTGTGTTCC AGGATGAGAT CGTCATAGGC CATTTTTACA AAGCGCGGGC
3241 GGAGGGTGCC AGACTGCGGT ATAATGGTTC CATCCGGCCC AGGGGCGTAG TTACCCTCAC
3301 AGATTTGCAT TTCCCACGCT TTGAGTTCAG ATGGGGGGAT CATGTCTACC TGCGGGGCGA
3361 TGAAGAAAAC GGTTTCCGGG GTAGGGGAGA TCAGCTGGGA AGAAAGCAGG TTCCTGAGCA
3421 GCTGCGACTT ACCGCAGCCG GTGGGCCCGT AAATCACACC TATTACCGGG TGCAACTGGT
3481 AGTTAAGAGA GCTGCAGCTG CCGTCATCCC TGAGCAGGGG GGCCACTTCG TTAAGCATGT
3541 CCCTGACTCG CATGTTTTCC CTGACCAAAT CCGCCAGAAG GCGCTCGCCG CCCAGCGATA
3601 GCAGTTCTTG CAAGGAAGCA AAGTTTTTCA ACGGTTTGAG ACCGTCCGCC GTAGGCATGC
3661 TTTTGAGCGT TTGACCAAGC AGTTCCAGGC GGTCCCACAG CTCGGTCACC TGCTCTACGG
3721 CATCTCGATC CAGCATATCT CCTCGTTTCG CGGGTTGGGG CGGCTTTCGC TGTACGGCAG
3781 TAGTCGGTGC TCGTCCAGAC GGGCCAGGGT CATGTCTTTC CACGGGCGCA GGGTCCTCGT
3841 CAGCGTAGTC TGGGTCACGG TGAAGGGGTG CGCTCCGGGC TGCGCGCTGG CCAGGGTGCG
3901 CTTGAGGCTG GTCCTGCTGG TGCTGAAGCG CTGCCGGTCT TCGCCCTGCG CGTCGGCCAG
3961 GTAGCATTTG ACCATGGTGT CATAGTCCAG CCCCTCCGCG GCGTGGCCCT TGGCGCGCAG
4021 CTTGCCCTTG GAGGAGGCGC CGCACGAGGG GCAGTGCAGA CTTTTGAGGG CGTAGAGCTT
4081 GGGCGCGAGA AATACCGATT CCGGGGAGTA GGCATCCGCG CCGCAGGCCC CGCAGACGGT
4141 CTCGCATTCC ACGAGCCAGG TGAGCTCTGG CCGTTCGGGG TCAAAAACCA GGTTTCCCCC
4201 ATGCTTTTTG ATGCGTTTCT TACCTCTGGT TTCCATGAGC CGGTGTCCAC GCTCGGTGAC
4261 GAAAAGGCTG TCCGTGTCCC CGTATACAGA CTNNNGTTTA AACGAATTCN NNATATAAAA
4321 TGCAAGGTGC TGCTCAAAAA ATCAGGCAAA GCCTCGCGCA AAAAAGAAAG CACATCGTAG
4381 TCATGCTCAT GCAGATAAAG GCAGGTAAGC TCCGGAACCA CCACAGAAAA AGACACCATT
4441 TTTCTCTCAA ACATGTCTGC GGGTTTCTGC ATAAACACAA AATAAAATAA CAAAAAAACA
4501 TTTAAACATT AGAAGCCTGT CTTACAACAG GAAAAACAAC CCTTATAAGC ATAAGACGGA
4561 CTACGGCCAT GCCGGCGTGA CCGTAAAAAA ACTGGTCACC GTGATTAAAA AGCACCACCG
4621 ACAGCTCCTC GGTCATGTCC GGAGTCATAA TGTAAGACTC GGTAAACACA TCAGGTTGAT
4681 TCATCGGTCA GTGCTAAAAA GCGACCGAAA TAGCCCGGGG GAATACATAC CCGCAGGCGT
4741 AGAGACAACA TTACAGCCCC CATAGGAGGT ATAACAAAAT TAATAGGAGA GAAAAACACA
4801 TAAACACCTG AAAAACCCTC CTGCCTAGGC AAAATAGCAC CCTCCCGCTC CAGAACAACA
4861 TACAGCGCTT CACAGCGGCA GCCTAACAGT CAGCCTTACC AGTAAAAAAG AAAACCTATT
4921 AAAAAAACAC CACTCGACAC GGCACCAGCT CAATCAGTCA CAGTGTAAAA AAGGGCCAAG
4981 TGCAGAGCGA GTATATATAG GACTAAAAAA TGACGTAACG GTTAAAGTCC ACAAAAAACA
5041 CCCAGAAAAC CGCACGCGAA CCTACGCCCA GAAACGAAAG CCAAAAAACC CACAACTTCC
5101 TCAAATCGTC ACTTCCGTTT TCCCACGTTA CGTAACTTCC CATTTTAAGA AAACTACAAT
5161 TCCCAACACA TACAAGTTAC TCCGCCCTAA AACCTACGTC ACCCGCCCCG TTCCCACGCC
5221 CCGCGCCACG TCACAAACTC CACCCCCTCA TTATCATATT GGCTTCAATC CAAAATAAGG
5281 TATATTATTG ATGATNNNTT AATTAAGGAT CCNNNCGGTG TGAAATACCG CACAGATGCG
5341 TAAGGAGAAA ATACCGCATC AGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT
5401 CGGTCGTTCG GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA
5461 CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA
5521 ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC
5581 ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG
5641 CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT
5701 ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT
5761 ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC
5821 AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG
5881 ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG
5941 GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG
6001 GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG
6061 GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA
6121 GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA
6181 ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA
6241 TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT
6301 CTGACAGTTA CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT
6361 CATCCATAGT TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT
6421 CTGGCCCCAG TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG
6481 CAATAAACCA GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT
6541 CCATCCAGTC TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT
6601 TGCGCAACGT TGTTGNNNNA AAAAGGATCT TCACCTAGAT CCTTTTCACG TAGAAAGCCA
6661 GTCCGCAGAA ACGGTGCTGA CCCCGGATGA ATGTCAGCTA CTGGGCTATC TGGACAAGGG
6721 AAAACGCAAG CGCAAAGAGA AAGCAGGTAG CTTGCAGTGG GCTTACATGG CGATAGCTAG
6781 ACTGGGCGGT TTTATGGACA GCAAGCGAAC CGGAATTGCC AGCTGGGGCG CCCTCTGGTA
6841 AGGTTGGGAA GCCCTGCAAA GTAAACTGGA TGGCTTTCTC GCCGCCAAGG ATCTGATGGC
6901 GCAGGGGATC AAGCTCTGAT CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG
6961 ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG
7021 CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC
7081 CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAA GACGAGGCAG
7141 CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA
7201 CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT
7261 CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA
7321 CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC
7381 GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC
7441 TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT GCCCGACGGC GAGGATCTCG
7501 TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG
7561 GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA
7621 CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG
7681 GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT
7741 GAATTTTGTT AAAATTTTTG TTAAATCAGC TCATTTTTTA ACCAATAGGC CGAAATCGGC
7801 AACATCCCTT ATAAATCAAA AGAATAGACC GCGATAGGGT TGAGTGTTGT TCCAGTTTGG
7861 AACAAGAGTC CACTATTAAA GAACGTGGAC TCCAACGTCA AAGGGCGAAA AACCGTCTAT
7921 CAGGGCGATG GCCCACTACG TGAACCATCA CCCAAATCAA GTTTTTTGCG GTCGAGGTGC
7981 CGTAAAGCTC TAAATCGGAA CCCTAAAGGG AGCCCCCGAT TTAGAGCTTG ACGGGGAAAG
8041 CCGGCGAACG TGGCGAGAAA GGAAGGGAAG AAAGCGAAAG GAGCGGGCGC TAGGGCGCTG
8101 GCAAGTGTAG CGGTCACGCT GCGCGTAACC ACCACACCCG CGCGCTTAAT GCGCCG
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关实验
pCF-T M13F M13R pCI T7（17Base） 此载体无反向引物 pCI-neo T7（17Base） T3 pCMS-EGFP T7 T3 pCMV-3Tag-4A T3 /PFLAG-CMV-F T7 pCMV5 pCMV5F pCMV5R pCMV5-Flag pCMV5F pCMV5R pCMV-MYC/HA pCMV-F pCMV-R pCMV-Sport M13F/T7 SP6/M13R pCMV-Tag(KAN+) T3 T7 pCR2.1-TOPO
naj2007 请教一下各位老师，在构建过表达载体的时候，VEGF和GFP是不是不可以做成融合蛋白，但是如果用不同的启动子的话，很容易丢失荧光，有没有什么解决方法。 zhujoker 可以做成融合蛋白的，即使是使用不同的启动子的话荧光也不那么容易丢失的，大不了你将荧光的启动子换成强的如CMV，EF1a之类。 kinguangjun 或者你可以用CMV-VEGF-IRES-GFP来做
靶向神经元的血清型⸺AAV-EP+ 神经元作为中枢神经系统的核心功能单元，负责信息处理、传递和存储，是维持认知、运动和感觉功能的基础。随着阿尔茨海默 病、帕金森病、亨廷顿病等神经退行性疾病发病率的不断上升，神经元已成为基因治疗领域的重要靶点。然而，针对大脑神经元 的基因递送面临着独特的挑战：血脑屏障的严密保护、神经元亚型的高度异质性、以及对外源载体的敏感性都限制了传统基因 治疗载体的应用效果。传统 AAV 血清型在跨越血脑屏障和实现神经元特异性转导方面存在效率低、脱靶效应明显等问题。 2025 年 5 月
技术资料暂无技术资料 索取技术资料
pShuttle-CMV-EGFP-C载体
¥1600
