| 出品公司: | ZYbscience |
|---|---|
| 载体名称: | pRI101-AN |
| 质粒类型: | 植物载体；农杆菌双元表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | 35S |
| 载体大小: | 10417 bp |
| 5' 测序引物及序列: | 35S promoter 5’CTATCCTTCGCAAGACCCTTC 3’ |
| 3' 测序引物及序列: | M13 Reverse 5’CAGGAAACAGCTATGAC 3’ |
| 载体标签: | 无 |
| 载体抗性: | 卡那霉素 |
| 筛选标记: | 卡那霉素 |
| 克隆菌株: | HB101 |
| 宿主细胞（系）: | 植物细胞、农杆菌 |
| 备注: | pRI101-AN载体是一种在双子叶植物细胞中表达外源基因的载体。利用发根土壤杆菌介导。 |
| 产品目录号: | ZY3262 |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
- ¥2000
- ZYbscience
- 中国/美国
- ZY3262
- 2025年07月09日
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- 详细信息
- 技术资料
- 保存条件：
-20℃低温保存
- 保质期：
三年
- 英文名：
pRI101-AN
- 库存：
20
- 供应商：
泽叶生物
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
pRI101-AN载体简介：
pRI101 DNA vectors are binary plant transformation vectors intended for foreign gene
expression in plant cells. These agrobacterium-mediated plant transformation vectors carry
the 35S promoter of caμliflower mosaic virus (CaMV) and the 5’ non-coding region (5’-UTR)
of the alcohol dehydrogenase (ADH) gene. The 5’-UTR of ADH functions as a translation
enhancer in plants. There are two types of pRI101 DNA vectors, the pRI101-AN DNA vector,
which carries the 5’-UTR of Arabidopsis ADH (AtADH 5’-UTR), and the pRI101 ON DNA vector,
which carries the 5’-UTR of rice ADH (OsADH 5’-UTR). The pRI101-AN DNA vector is for
dicotyledonous plants such as tobacco or Arabidopsis while the pRI101-ON DNA vector is for
monocotyledonous plants such as rice.
Although the pRI101 DNA vectors are shuttle vectors and replicate autonomously in E. coli and
Rhizobium (Agrobacterium), they are high copy number plasmids because they contain the same
replication origin as pUC-type plasmids (ColE1 ori). These vectors are also stably maintained
in Rhizobium (Agrobacterium) containing the mutant-type replication origin Ri (Ri-ori). The
pRI101 DNA vectors are capable of stably integrating target genes into plant chromosomes
because the vector cloning sites are located closer to the Right Border (RB) of T-DNA than
the selection marker (NPT II) of the plant, so the target gene is not deleted.
pRI101-AN载体特征：
Agrobacterium-mediated plant transformation
Easy and stable integration of dicot and monocot target genes into plant chromosomes
Increased target gene expression via the 5'-UTR translation enhancer region
Vector versatility for dicotyledonous and monoctyledonous plant cells
参考文献：
1. Sugio, T., et al. (2008) J. Bioscience and Bioengineering 105(3):300-302.
2. Nishiguchi, R. et al. (1987) Mol. and Gen. Genet. 206:1-8.
3. Satoh, J. et al. (2004) J. Bioscience and Bioengineering 98(1):1-8.
载体序列
TGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTC CATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGA AACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCT CCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTG GCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAG CTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTAT CGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAAC AGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAAC TACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTC GGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTT TTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATC TTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATG AGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCA ATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCA CCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCGATCCTACAAGG TAGAATCCGCCTGAGTCGCAAGGGTGACTTCGCCTATATTGGACGACGGCGCGCAGAGGG CGACCTCTTTTTGGGTTACGATTGTAGGATTATCACTAAAACAATACATGAACATATTCA AATGGCAATCTCTCTAAGGCATTGGAAATAAATACAAATAACAGTTGGGTGGAGTTTTTC GACCTGAGGGCGTTAACCTTCTGTTAACCTAAAAGCTCTTGCCCAAACAGCAGAATCGGC GCTAATTGCCAGCGGCGGAACTTTTCCAGTTTCGCGAAAAATATCGCCACTGGCAAGGAA TGGGTTTGAGATGGCGAAGTCTGTCCTAAAAGCAGCGCCTGTAGTTGTAGGGTTGACGGC CTTGATGGAGCGTCATGCCGATGCCCTCTCGAGCCAACTTCAAGCACATCATCTTAAGGT TTTCCCGCCGCATTCCGAGAAGGGTATTCGAACATTCGGGCCATCGGAGGCGTCCAAGCT GCTCGGCGTTGGCGAGTCATATTTACGGCAGACCGCGTCTGAGATGCCAGAGTTGAATGT TAGCATGAGCCCAGGTGGCAGGCGAATGTTCTCAATTGAAGATATCCATGTGATTCGGAA GTATATGGATCAGGTCGGCCGCGGGAACCGGCGCTACCTGCCACATCGTCGAGGCGGCGA GCAGCTTCAGGTTATCTCTGTGATGAATTTCAAAGGTGGGTCGGGTAAGACCACCACCGC CGCGCATCTGGCGCAGTACCTCGCTATGCGCGGATATCGAGTCTTGGCCATTGATCTCGA TCCTCAAGCGAGCCTTTCTGCACTCTTTGGGAGCCAACCGGAGACGGACGTTGGCCCGAA CGAAACGCTCTACGGCGCTATAAGGTATGATGATGAGCAGGTGGCAATCGAACGAGTCGT CCGAGGGACTTACATTCCCGACCTCCACCTGATTCCTGGTAACCTTGAGCTGATGGAGTT TGAACACGATACGCCACGCGCGCTGATGAACCGCAAAGAGGGCGACACGCTCTTTTATGG TCGCATCAGCCAAGTAATTGAAGATATCGCGGATAACTATGACGTCGTGGTCATCGACTG CCCTCCCCAGCTTGGGTATCTCACGCTATCCGCATTGACTGCGGCGACGTCCATTCTTGT CACGGTCCATCCGCAGATGCTGGATGTGATGTCGATGAACCAGTTTCTGGCAATGACATC GAACCTTTTGCGTGAAATCGAGAATGCTGGCGCCAAGTTCAAGTTTAATTGGATGCGCTA TCTGATAACCCGTTTCGAACCGAGCGACGGACCACAGAACCAAATGGTAGGTTATCTGCG GTCGATTTTTGGCGAAAATGTCCTCAATTTTCCGATGCTTAAAACCACCGCGGTTTCGGA CGCTGGCCTGACAAACCAGACTCTATTCGAAGTGGAGCGTGGCCTGTTCACGCGCTCGAC CTATGATCGAGCCTTGGAGGCGATGAACGCCGTCAACGACGAGATCGAAACACTGATCAA AAAAGCATGGGGTAGGCCCACATGAGCCGGAAGCACATCCTTGGCGTCTCAACTGACGCC CCTGAGACGTCGCCCGCCGACAATAGGACGGCAAAGAACCGCTCCATGCCGCTCCTCGGC GTAACAAGGAAGGAGCGCGATCCGGCAACGAAGCTCACAGCGAACATTGGTAACGCACTG CGAGAGCAAAACGATCGTCTTAGCCGTGCCGAAGAGATCGAGCGGCGTCTCGCTGAAGGT CAGGCAGTGATAGAGTTGGATGCCTCGTCAATAGAACCGTCTTTCGTGCAGGATCGTATG CGAGGGGACATTGACGGGCTCCTTACTTCGATCCGGGAACAAGGACAGCAAGTCCCAATC CTTGTGCGACCGCATCCGAGCCAGCCGGGCCGATATCAGGTTGCCTTCGGCCACCGCCGG CTACGCGCCGTTTCAGAACTCGGACTTCCGGTCAGGGCGGTCGTTCGCGAACTGACGGAC GAGCAAGTGGTCGTAGCACAGGGTCAGGAAAACAATGTGCGCGAAGATCTTACCTTCATC GAAAAGGCGCGCTTCGCACATCGCCTGAACAGGCAGTTTTCTCGAGAGATTGTCATCGCC GCGATGTCGATCGACAAGAGCAATTTGTCCAAGATGCTTCTGCTCGTTGACGCCCTCCCC TCTGAACTGACCGATGCTATTGGTGCCGCTCCTGGTGTTGGACGGCCGAGTTGGCAACAA CTTGCCGAGCTGATTGAGAAAGTTTCTTCACCGGCCGACGTGGCTAAATATGCTATGTCG GAGGAAGTTCAAGCGCTGCCATCGGCAGAACGATTCAAGGCGGTGATCGCTAGTCTGAAG CCCAGTCGGGTTGCGCGTGGACTTCCCGAGGTCATGGCCACCCCAGACGGCACCAGAATT GCACAGGTGACGCAGAGCAAGGCCAAACTGGAAATCACGATTGACAGGAAGGCGACGCCC GATTTTGCGACCTTCGTGCTCGATCATGTGCCAGCGCTGTATCAAGCGTACCACGCTGAG AACCAACGGAAACGGGGAGAGTAAACCGCAAAAGAAAAGAGCCCCCTCAACGTCGCCGTC GCGGAAGCCCTTCTGTCTCTCTAGCGCGAACAGAATCGCATTTCCTCGAATCCTCGTCAA GAGTTTTTAGCGCCGTTTTGGTGAGCTGATTTCCTTTGCCTGCTGAAAGGTGAAAGATGA TGCAGACAGGAAGTGTAACGACGCCATTCGGGCGGCGGCCAATGACGCTTGCGCTTGTGC GGCGCCAGACGGCGCTGGCCGATATCAAACAAGGCAAGACAGCGGACAAGTGGAAGGTCT TTAGGGACGCGTCCGCGGCCATGGAACTACTTGGAATCCAGTCCAACAGTCTTGCCGTCC TTGATGCGCTATTGAGCTTTCACCCGGAAACGGAGTTGCGTCAGGAGGCACAGCTGATCG TCTTCCCGTCGAATGCTCAGCTTGCCCTTCGGGCGCATGGGATGGCTGGCGCGACTTTGC GTAGGCACATCGCCATGCTCGTGGAGTCAGGCTTGATCGTCCGGAAGGATAGCGCCAACG GAAAGCGTTACGCTCGTAAGGATGGCGCTGGTCAGATCGAGCGCGCGTTTGGCTTCGATT TGTCTCCGCTTCTCGCGCGGTCCGAAGAGCTAGCGATGATGGCACAGCAGGTGATGGCCG ATCGAGCAGCATTCAGGATGGCCAAAGAAAGTCTGACGATTTGCCGACGGGACGTTCGGA AGCTAATTACGGCAGCTATGGAAGAGGGAGCGGAGGGCGACTGGCAAGCTGTCGAGGAAG TCTATGTGGAACTTGTGGGTAGAATCCCACGCGCCCCGACGCTTGCTGATGTAGAGTCAA TTCTCGAAGAGATGTGGATGCTCCAGGAAGAGATAATCAACCGGTTGGAAATTAGAGACA ATTCAGAAAATAATAGCACCAATGCTGCCCAGAGCGAGCAGCACATACAGAACTCAAAAC CCGAATCCGTTAATGAACTTGAACCTCGCTCTGAAAAGGAGCAGGGCGCTAAGCCGAGTG AAATAGACCGGGCAAGGAGCGAGCCGATAAAAGCGTTCCCCCTCGGGATGATCCTGAAAG CGTGCCCGACCATTGGCAATTATGGGCCGAGCGGTGCGGTTGCTAGCTGGCGTGACCTCA TGTCGGCTGCGGTGGTGGTTCGGTCTATGCTGGGGGTCAGCCCGTCGGCTTACCAAGACG CGTGTGAGGCAATGGGACCGGAGAATGCGGCAGCAGCGATGGCGTGCATTTTGGAGCGAG CGAACTTCATCAATTCGCCTGGGGGCTATCTCCGAGATCTGACACGGCGGAGCGAACTCG GGAAGTTTTCACTTGGCCCGATGATAATGGCGCTCTTGAAGGCTAGCGGGCAGGGGACGT TGCGGTTTGGCTAGAATTAGCGAGTATGGAGCAGGATGGTCTGTGGTCAGCTGACCACAG ACCTAATAGGTTGAAAACATGAGCGTTTTTTGGATGATCGACAGACCATCCGATTCCCGG AGTACCAAGCGTGCTCTGATGGGAGCGATAACATTACTCAACAAGCACGAAGGCCCCATG CCGATCGTTGATCGTGAAGGAGAGCCTGCTCTACATGCGGCGGTATTTTGCCGGCCGAGG CATGTAGTCGCGGAGCACTGCCTATTTACTGCCCTAGGCACAAACGTTGACTCTTGGATC GAGCTGGCAGACAAAGCAATAACCCACACAGAGGACGATTAATGGCTGACGAAGAGATCC AGAATCCGCCGGACGGTACTGCTGCTGCCGAAGTTGAGCCGGCTGCTCCTAGAGGTAGAA GAGCAAAGAAAGCACCAGCCGAAACAGCCCGCACGGGATCGTTCAAATCCGTGAAGCCGA AAACCCGCGGCCTCAGCAACCGAGAAAAACTGGAGAAGATCGGTCAAATCGAAGCTCAGG TCGCTGGCGGCGCAACCTTGAAGGACGCCGTTAAGATCGTGGGTATTTCCGTTCAGACCT ATTATCAATGGAAGAGAGCTGCGGTTCAACCTGTCTCACAGAATCCGGCCGTGTCTGTTT CAGTTGACGATGAACTCGGCGAGTTCATCCAACTCGAGGAGGAAAATCGGCGGCTCAGAA AGCTAGCTTGTGGCAGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATA ACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGC AACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTG GTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAAATCGGCAAAATCCCTT ATAAATCAAAAGAATAGCCCGAGATAGGGTTGAGTGTTGTTCCAGTTTGGAACAAGAGTC CACTATTAAAGAACGTGGACTCCAACGTCAAAGGGCGAAAAACCGTCTATCAGGGCGATG GCCCAAATTCCCGATCTAGTAACATAGATGACACCGCGCGCGATAATTTATCCTAGTTTG CGCGCTATATTTTGTTTTCTATCGCGTATTAAATGTATAATTGCGGGACTCTAATCATAA AAACCCATCTCATAAATAACGTCATGCATTACATGTTAATTATTACATGCTTAACGTAAT TCAACAGAAATTATATGATAATCATCGCAAGACCGGCAACAGGATTCAATCTTAAGAAAC TTTATTGCCAAATGTTTGAACGATCGGGGCCCGATCATATTGTCGCTCAGGATCGTGGCG TTGTGCTTGTCGGCCGTTGCTGTCGTAATGATATCGGCACCTTCGACCGCCTGTTCCGCA
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pRI101-AN载体
¥2000
