pYC50载体

LOCUS       pYC50	6481 bp 	DNA	SYN
DEFINITION  pYC50
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..6481
                     /organism="pYC50"
                     /mol_type="other DNA"
     misc_feature    complement(29..51)
                     /label="pGEX_3_primer"
     promoter        196..409
                     /label="URA3_promoter"
     gene            411..1211
                     /label="URA3"
                     /gene="URA3"
     CDS             411..1214
                     /label="ORF frame 3"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
                     LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
                     LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
                     TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
                     TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN*"
     CDS             complement(446..922)
                     /label="ORF frame 1"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     misc_feature    complement(1814..1962)
                     /label="lacZ_a"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     misc_feature    1928..1950
                     /label="M13_pUC_fwd_primer"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     promoter        1943..1959
                     /label="M13_forward20_primer"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     promoter        1966..1984
                     /label="T7_promoter"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     misc_feature    2017..2033
                     /label="pBluescriptSK_primer"
                     /translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
                     GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
                     EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
     CDS             complement(3287..3790)
                     /label="ORF frame 1"
                     /translation="MNSWSINIGNFQPQMTSTSFSGEFSSQTHQSSTNTMTSMFWSNF
                     NIFNSQSSVPTRIRNNNETSQITIITISNESSRIWITIFTSIGFIIGFIIWENLSQWW
                     INWNFSQSETITSSSNSKNSIGSERTIQSFNSFSITWNRSSVSISSIIQSSRHNCRID
                     PVVYVRM*"
     CDS             3326..3898
                     /label="ORF frame 2"
                     /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                     FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                     NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
                     GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
     promoter        complement(4220..4239)
                     /label="T3_promoter"
                     /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                     FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                     NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
                     GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
     promoter        complement(4256..4274)
                     /label="M13_reverse_primer"
                     /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                     FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                     NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
                     GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
     misc_feature    complement(4273..4295)
                     /label="M13_pUC_rev_primer"
                     /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                     FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                     NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
                     GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
     rep_origin      complement(4647..5266)
                     /label="pBR322_origin"
                     /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                     FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                     NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
                     GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
     gene            complement(5421..6281)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG
                     FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGW
                     NRRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRM
                     GFTLCGLDTALYDGTASDGEQALYMSMPCP*"
     CDS             complement(5421..6281)
                     /label="ORF frame 3"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(6323..6351)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 Tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
   61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
  121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
  181 accacgcttt tcaattcaat tcatcatttt ttttttattc ttttttttga tttcggtttc
  241 tttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca
  301 gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat
  361 tcttaaccca actgcacaga acaaaaacct gcaggaaacg aagataaatc ATGTCGAAAG
  421 CTACATATAA GGAACGTGCT GCTACTCATC CTAGTCCTGT TGCTGCCAAG CTATTTAATA
  481 TCATGCACGA AAAGCAAACA AACTTGTGTG CTTCATTGGA TGTTCGTACC ACCAAGGAAT
  541 TACTGGAGTT AGTTGAAGCA TTAGGTCCCA AAATTTGTTT ACTAAAAACA CATGTGGATA
  601 TCTTGACTGA TTTTTCCATG GAGGGCACAG TTAAGCCGCT AAAGGCATTA TCCGCCAAGT
  661 ACAATTTTTT ACTCTTCGAA GACAGAAAAT TTGCTGACAT TGGTAATACA GTCAAATTGC
  721 AGTACTCTGC GGGTGTATAC AGAATAGCAG AATGGGCAGA CATTACGAAT GCACACGGTG
  781 TGGTGGGCCC AGGTATTGTT AGCGGTTTGA AGCAGGCGGC AGAAGAAGTA ACAAAGGAAC
  841 CTAGAGGCCT TTTGATGTTA GCAGAATTGT CATGCAAGGG CTCCCTATCT ACTGGAGAAT
  901 ATACTAAGGG TACTGTTGAC ATTGCGAAGA GCGACAAAGA TTTTGTTATC GGCTTTATTG
  961 CTCAAAGAGA CATGGGTGGA AGAGATGAAG GTTACGATTG GTTGATTATG ACACCCGGTG
 1021 TGGGTTTAGA TGACAAGGGA GACGCATTGG GTCAACAGTA TAGAACCGTG GATGATGTGG
 1081 TCTCTACAGG ATCTGACATT ATTATTGTTG GAAGAGGACT ATTTGCAAAG GGAAGGGATG
 1141 CTAAGGTAGA GGGTGAACGT TACAGAAAAG CAGGCTGGGA AGCATATTTG AGAAGATGCG
 1201 GCCAGCAAAA CTAAaaaact gtattataag taaatgcatg tatactaaac tcacaaatta
 1261 gagcttcaat ttaattatat cagttattac cctgcggtgt gaaataccgc acagatgcgt
 1321 aaggagaaaa taccgcatca ggaaattgta aacgttaata ttttgttaaa attcgcgtta
 1381 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
 1441 aaatcaaaag aatagaccga gatagggttg agtgTTGTTC CAGTTTGGAA CAAGAGTCCA
 1501 CTATTAAAGA ACGTGGACTC CAACGTCAAA GGGCGAAAAA CCGTCTATCA GGGCGATGGC
 1561 CCACTACGTG AACCATCACC CTAATCAAGT TTTTTGGGGT CGAGGTGCCG TAAAGCACTA
 1621 AATCGGAACC CTAAAGGGAG CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG
 1681 GCGAGAAAGG AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG
 1741 GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG Cgccgctaca gggcgcgtcg
 1801 cgCCATTCGC CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG
 1861 CTATTACGCC AGCTGGCGAA GGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA
 1921 GGGTTTTCCC AGTCACGACG TTGTAAAACG ACGGCCAGTG AAttgtaata cgactcacta
 1981 tagggcgaat tggagctcca ccgcggtggc ggccgctcta gaactagtgg atcctagggT
 2041 TAATTAACGG TGAACAAAAG CTAATCTCCG AGGAAGACTT GAACGGTGAA CAAAAATTAA
 2101 TCTCAGAAGA AGACTTGAAC GGACTCGACG GTGAACAAAA GTTGATTTCT GAAGAAGATT
 2161 TGAACGGTGA ACAAAAGCTA ATCTCCGAGG AAGACTTGAA CGGTGAACAA AAATTAATCT
 2221 CAGAAGAAGA CTTGAACGGA CTCGACGGTG AACAAAAGTT GATTTCTGAA GAAGATTTGA
 2281 ACGGTGAACA AAAGCTAATC TCCGAGGAAG ACTTGAACGG TGAACAAAAA TTAATCTCAG
 2341 AAGAAGACTT GAACGGACTC GACGGTGAAC AAAAGTTGAT TTCTGAAGAA GATTTGAACG
 2401 GTGAACAAAA GCTAATCTCC GAGGAAGACT TGAACGGTGA ACAAAAATTA ATCTCAGAAG
 2461 AAGACTTGAA CGGACTCGAC GGTGAACAAA AGTTGATTTC TGAAGAAGAT TTGAACGGTG
 2521 AACAAAAGCT AATCTCCGAG GAAGACTTGA ACGGTGAACA AAAATTAATC AATCACtagc
 2581 tagtgaattc gcgccagatc cGAAGTTCCT ATACTTTCTA GAGAATAGGA ACTTCgtgcg
 2641 cttttgaacc acgtaaagtg ctgtaaaaca gctctattca atgaggtaaa tgataaagtt
 2701 tataagagac caaaaaattc aaaaaaaaaa ttgaaaaatt atattggtat ttatatgaaa
 2761 caatagttca gttcaaagcg ctagcttttc tcatctcaac taaccggtat taggtgggta
 2821 tttatttaat atgggaacaa gaaatgtgac actaatgttc atgtttttat tttcattatt
 2881 tattctatat ggtttgggac ttgatgttat gtcttaattc atagaGATCC gcttgcctcg
 2941 tccccgccgg gtcacccggc cagcgacatg gaggcccaga ataccctcct tgacagtctt
 3001 gacgtgcgca gctcaggggc atgatgtgac tgtcgcccgt acatttagcc catacatccc
 3061 catgtataat catttgcatc catacatttt gatggccgca cggcgcgaag caaaaattac
 3121 ggctcctcgc tgcagacctg cgagcaggga aacgctcccc tcacagacgc gttgaattgt
 3181 ccccacgccg cgcccctgta gagaaatata aaaggttagg atttgccact gaggttcttc
 3241 tttcatatac ttccttttaa aatcttgcta ggatacagtt ctcacatcac atccgaacat
 3301 aaacaaccgG ATCgatccta caattATGTC TACTACTTTG GATGATACTG CTTATAGATA
 3361 CAGAACTTCT GTTCCAGGTG ATGCTGAAGC TATTGAAGCT TTGGATGGTT CTTTCACTAC
 3421 CGATACTGTT TTTAGAGTTA CTGCTACTGG TGATGGTTTC ACTTTGAGAG AAGTTCCAGT
 3481 TGATCCACCA TTGACTAAGG TTTTCCCAGA TGATGAATCC GATGATGAAT CCGATGCTGG
 3541 TGAAGATGGT GATCCAGATT CTAGAACTTT CGTTGCTTAT GGTGATGATG GTGATTTGGC
 3601 TGGTTTCGTT GTTGTTTCTT ATTCTGGTTG GAACAGAAGA TTGACTGTTG AAGATATTGA
 3661 AGTTGCTCCA GAACATAGAG GTCATGGTGT TGGTAGAGCT TTGATGGGTT TGGCTACTGA
 3721 ATTCGCCAGA GAAAGAGGTG CTGGTCATTT GTGGTTGGAA GTTACCAATG TTAATGCTCC
 3781 AGCTATTCAT GCTTATAGAA GAATGGGTTT CACTTTGTGT GGTTTGGATA CTGCTTTATA
 3841 CGATGGTACT GCTTCCGATG GTGAACAAGC TTTGTATATG TCTATGCCAT GTCCATAAta
 3901 acTCGACcag tactgacaat aaaaagattc ttgttttcaa gaacttgtca tttgtatagt
 3961 ttttttatat tgtagttgtt ctattttaat caaatgttag cgtgatttat attttttttc
 4021 gcctcgacat catctgccca gatgcgaagt taagtgcgca gaaagtaata tcatgcgtca
 4081 atcgtatgtg aatgctggtc gctatactgc tgtcgattcg atactaacgc cgccatccag
 4141 tgTCGAtcga cGAAGTTCCT ATACTTTCTA GAGAATAGGA ACTTCGTCct cgaggggggg
 4201 cccggtacca gcttttgttc cctttagtga gggttaattc cgagcttggc gtaatcatgg
 4261 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa cataggagcc
 4321 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga ggtaactcac attaattgcg
 4381 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
 4441 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
 4501 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
 4561 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
 4621 caaaaggcca ggaaccgtaa aaaggcCGCG TTGCTGGCGT TTTTCCATAG GCTCGGCCCC
 4681 CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA
 4741 TAAAGATACC AGGCGTTCCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG
 4801 CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCAATGC
 4861 TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC
 4921 GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC
 4981 CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG
 5041 AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA
 5101 AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT
 5161 AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG
 5221 CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTtttc tacggggtct
 5281 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
 5341 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
 5401 gagtaaactt ggtctgacag TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC
 5461 TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTGCCCGTCG TGTAGATAAC TACGATACGG
 5521 GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC GAGACCCACG CTCACCGGCT
 5581 CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA
 5641 ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG
 5701 CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG
 5761 TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT TACATGATCC
 5821 CCCATGTTGT GAAAAAAAGC GGTTAGCTCC TTCGGTCCTC CGATCGTTGT CAGAAGTAAG
 5881 TTGGCCGCAG TGTTATCACT CATGGTTATG GCAGCACTGC ATAATTCTCT TACTGTCATG
 5941 CCATCCGTAA GATGCTTTTC TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG
 6001 TGTATGCGGC GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT
 6061 AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA ACTCTCAAGG
 6121 ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC GTGCACCCAA CTGATCTTCA
 6181 GCATCTTTTA CTTTCACCAG CGTTTCTGGG TGAGCAAAAA CAGGAAGGCA AAATGCCGCA
 6241 AAAAAGGGAA TAAGGGCGAC ACGGAAATGT TGAATACTCA Tactcttcct ttttcaatat
 6301 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
 6361 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
 6421 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
 6481 c
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