| 出品人: | ZYbscience） |
|---|---|
| 载体名称: | pYC121 |
| 质粒类型: | 酿酒酵母载体；复制型载体；过表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | TEF1 |
| 载体大小: | 6277 bp |
| 5' 测序引物及序列: | M13/pUC fwd primer 5’ CCAGTCACGACGTTGTAAAACG 3’ |
| 3' 测序引物及序列: | -- |
| 载体标签: | Myc tag |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | URA3 |
| 克隆菌株: | DH10B感受态细胞 ,30°C |
| 宿主细胞（系）: | 出芽酵母（酿酒酵母）、白色念珠菌 |
| 备注: | -- |
| 产品目录号: | ZY63917 |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃低温保存
- 保质期：
三年
- 英文名：
pYC121
- 库存：
20
- 供应商：
泽叶生物
载体基本信息
载体质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pYC121 6277 bp DNA SYN
DEFINITION pYC121
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..6277
/organism="pYC121"
/mol_type="other DNA"
promoter 668..696
/label="AmpR_promoter"
gene 738..1598
/label="Ampicillin"
/gene="Ampicillin"
CDS 738..1598
/label="ORF frame 3"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
rep_origin 1753..2372
/label="pBR322_origin"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature 2724..2746
/label="M13_pUC_rev_primer"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter 2745..2763
/label="M13_reverse_primer"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter 2780..2799
/label="T3_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature 3218..3234
/label="pBluescriptSK_primer"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature complement(3853..3869)
/label="pBluescriptKS_primer"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
CDS 4105..4689
/label="ORF frame 1"
/translation="MSLWREIKYIKQLVSSASCGSGYSCNIWKNRKSRLLPFFSYYRG
RNTHEHDIEVKVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNS
EEARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSF
LATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
promoter complement(4283..4301)
/label="T7_promoter"
/translation="MSLWREIKYIKQLVSSASCGSGYSCNIWKNRKSRLLPFFSYYRG
RNTHEHDIEVKVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNS
EEARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSF
LATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
promoter complement(4311..4327)
/label="M13_forward20_primer"
/translation="MSLWREIKYIKQLVSSASCGSGYSCNIWKNRKSRLLPFFSYYRG
RNTHEHDIEVKVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNS
EEARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSF
LATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
misc_feature complement(4320..4342)
/label="M13_pUC_fwd_primer"
/translation="MSLWREIKYIKQLVSSASCGSGYSCNIWKNRKSRLLPFFSYYRG
RNTHEHDIEVKVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNS
EEARTDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSF
LATFAGFPRQALNRGLPLGFRFSALRHLDPKKLD*"
CDS complement(5058..5861)
/label="ORF frame 3"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN*"
gene complement(5061..5861)
/label="URA3"
/gene="URA3"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL
LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK
LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS
TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR
TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN*"
CDS 5350..5826
/label="ORF frame 1"
/translation="MSTVPLVYSPVDREPLHDNSANIKRPLGSFVTSSAACFKPLTIP
GPTTPCAFVMSAHSAILYTPAEYCNLTVLPMSANFLSSKSKKLYLADNAFSGLTVPSM
EKSVKISTCVFSKQILGPNASTNSSNSLVVRTSNEAHKFVCFSCMILNSLAATGLG*"
ORIGIN
1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
61 cttaGCAGGT TAGACTAACA TGCAAAAAGT TTATATTCAT TTTTGTAGTT ATTCTTGTTT
121 TCATTAGGAT GATTACACAC TGTGGATGTG GATTAATCGA TCATTGTTAA TTTAGTGTAG
181 CTACTACCAT TTCAAACAAA ACGTAAATTC TTGCAGTCGT ACTGGATCTG TGAATCTATT
241 AGTATATATG AATTAAAGTA GCTTGTACAT ATTATTCTGT TGAATCATAT CGCAGAGCAT
301 TGGTTGAAAT CCAAAATATA AAAATGTAAT ATCACAAAAA ATAATACTAA TTCTAACATT
361 AATGGGTCAG ATTTTTAGTG AATACTTAAA TTTATAATAT CGTCTATTTA AGCTAGCAAA
421 TGGAACAACA TTTAAAGTAA GAACATCATA TCTACATGAA AATGTATATT TCAATCTGAC
481 TAATAACGCA GAGCACATCT TTCAGTGATG TCTGTCACAT GATCAAAAAG AATTGTATTT
541 AAATAATTCA TAATAAAAGC TTAAAAAATT Acaataatga aaataaagtA AATAATGACA
601 TGGGTAAGAG TTCCGAAATA GAATCTTAGT GTACAAAACA AAAATTGCAT CATTAGAGAT
661 CCCCacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
721 attgaaaaag gaagagtATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG
781 CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG
841 AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC
901 TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT
961 GTGGCGCGGT ATTATCCCGT ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT
1021 ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA
1081 TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT
1141 TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG
1201 ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG
1261 AGCGTGACAC CACGATGCCT GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG
1321 AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG
1381 CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG
1441 CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC
1501 GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA
1561 TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAAct gtcagaccaa gtttactcat
1621 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
1681 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
1741 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
1801 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
1861 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
1921 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
1981 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
2041 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
2101 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
2161 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
2221 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
2281 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
2341 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
2401 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
2461 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
2521 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
2581 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
2641 caattaatgt gagttacctc actcattagg caccccaggc tttacacttt atgcttccgg
2701 ctcctatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
2761 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gAGCTCATAG
2821 CTTCAAAATG TTTCTACTCC TTTTTTACTC TTCCAGATTT TCTCGGACTC CGCGCATCGC
2881 CGTACCACTT CAAAACACCC AAGCACAGCA TACTAAATTT CCCCTCTTTC TTCCTCTAGG
2941 GTGTCGTTAA TTACCCGTAC TAAAGGTTTG GAAAAGAAAA AAGAGACCGC CTCGTTTCTT
3001 TTTCTTCGTC GAAAAAGGCA ATAAAAATTT TTATCACGTT TCTTTTTCTT GAAAATTTTT
3061 TTTTTGATTT TTTTCTCTTT CGATGACCTC CCATTGATAT TTAAGTTAAT AAACGGTCTT
3121 CAATTTCTCA AGTTTCAGTT TCATTTTTCT TGTTCTATTA CAACTTTTTT TACTTCTTGC
3181 TCATTAGAAA GAAAGCATAG CAATCTAATC TAAGTTTTCT AGaactagtg gatcctaggg
3241 TTAATTAACG GTGAACAAAA GCTAATCTCC GAGGAAGACT TGAACGGTGA ACAAAAATTA
3301 ATCTCAGAAG AAGACTTGAA CGGACTCGAC GGTGAACAAA AGTTGATTTC TGAAGAAGAT
3361 TTGAACGGTG AACAAAAGCT AATCTCCGAG GAAGACTTGA ACGGTGAACA AAAATTAATC
3421 TCAGAAGAAG ACTTGAACGG ACTCGACGGT GAACAAAAGT TGATTTCTGA AGAAGATTTG
3481 AACGGTGAAC AAAAGCTAAT CTCCGAGGAA GACTTGAACG GTGAACAAAA ATTAATCTCA
3541 GAAGAAGACT TGAACGGACT CGACGGTGAA CAAAAGTTGA TTTCTGAAGA AGATTTGAAC
3601 GGTGAACAAA AGCTAATCTC CGAGGAAGAC TTGAACGGTG AACAAAAATT AATCTCAGAA
3661 GAAGACTTGA ACGGACTCGA CGGTGAACAA AAGTTGATTT CTGAAGAAGA TTTGAACGGT
3721 GAACAAAAGC TAATCTCCGA GGAAGACTTG AACGGTGAAC AAAAATTAAT CAATCACtag
3781 ctagtgaatt cgcgccagat ccGAAGTTCC TATACTTTct aggggctgca ggaattcgat
3841 atcaagctta tcgataccgt cgacCTCGAG AACACAACCC ACAGCTACCA CCATCAACAA
3901 TATTTATATA TATAACGTAC ACATAGAAAT CACACAAACA GAGTATTTAT TCTTAACTAC
3961 ATGAACTACC ATCAGACCGT CTGGGCCACT ATATAATGTG CCATTCATAA ACGTGATCAC
4021 TTTACGTAGC AGGCAACCCC AGGTGAAAAT TTTTCAGCGA GCTGCCAGAT TGTCAGGTGA
4081 AAAACTGAAA AAAACTTCTG GGCGATGAGC TTGTGGCGGG AAATTAAGTA TATAAAGCAG
4141 TTAGTTTCTT CTGCTTCTTG TGGTTCTGGG TATTCTTGTA ATATTTGGAA GAATAGAAAA
4201 TCAAGACTAC TGCCTTTCTT TTCATATTAT AGAGGAAGAA ATACGCACGA ACACGATATA
4261 GAGGTAAAGG TACCcaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
4321 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
4381 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
4441 cagttgcgca gcctgaatgg cgaatggcgc gacgcgccct gtagcggcgc attaagcgcg
4501 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
4561 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
4621 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
4681 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
4741 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
4801 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
4861 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
4921 acaatttcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatag
4981 ggtaataact gatataatta aattgaagct ctaatttgtg agtttagtat acatgcattt
5041 acttataata cagttttTTA GTTTTGCTGG CCGCATCTTC TCAAATATGC TTCCCAGCCT
5101 GCTTTTCTGT AACGTTCACC CTCTACCTTA GCATCCCTTC CCTTTGCAAA TAGTCCTCTT
5161 CCAACAATAA TAATGTCAGA TCCTGTAGAG ACCACATCAT CCACGGTTCT ATACTGTTGA
5221 CCCAATGCGT CTCCCTTGTC ATCTAAACCC ACACCGGGTG TCATAATCAA CCAATCGTAA
5281 CCTTCATCTC TTCCACCCAT GTCTCTTTGA GCAATAAAGC CGATAACAAA ATCTTTGTCG
5341 CTCTTCGCAA TGTCAACAGT ACCCTTAGTA TATTCTCCAG TAGATAGGGA GCCCTTGCAT
5401 GACAATTCTG CTAACATCAA AAGGCCTCTA GGTTCCTTTG TTACTTCTTC TGCCGCCTGC
5461 TTCAAACCGC TAACAATACC TGGGCCCACC ACACCGTGTG CATTCGTAAT GTCTGCCCAT
5521 TCTGCTATTC TGTATACACC CGCAGAGTAC TGCAATTTGA CTGTATTACC AATGTCAGCA
5581 AATTTTCTGT CTTCGAAGAG TAAAAAATTG TACTTGGCGG ATAATGCCTT TAGCGGCTTA
5641 ACTGTGCCCT CCATGGAAAA ATCAGTCAAG ATATCCACAT GTGTTTTTAG TAAACAAATT
5701 TTGGGACCTA ATGCTTCAAC TAACTCCAGT AATTCCTTGG TGGTACGAAC ATCCAATGAA
5761 GCACACAAGT TTGTTTGCTT TTCGTGCATG ATATTAAATA GCTTGGCAGC AACAGGACTA
5821 GGATGAGTAG CAGCACGTTC CTTATATGTA GCTTTCGACA Tgatttatct tcgtttcctg
5881 caggtttttg ttctgtgcag ttgggttaag aatactgggc aatttcatgt ttcttcaaca
5941 ctacatatgc gtatatatac caatctaagt ctgtgctcct tccttcgttc ttccttctgt
6001 tcggagatta ccgaatcaaa aaaatttcaa agaaaccgaa atcaaaaaaa agaataaaaa
6061 aaaaatgatg aattgaattg aaaagctgtg gtatggtgca ctctcagtac aatctgctct
6121 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
6181 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagGtgcatg
6241 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcga
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文献和实验相关实验
质粒是存在于细菌染色体外的一个或多个能独立复制并稳定遗传的小型环状双链DNA分子，其分子量一般在0. 2～10kD 范围内。由于质粒分子小，便于分离和提取，可以携带目的基因进入细菌、动物细胞或植物体内进行扩增与表达。基因克隆实验中常将经改造后的质粒作为运送基因的载体，质粒D2NA提取效率及质量对后续实验步骤(如酶切、连接、转化大肠杆菌与PCR扩增等)的成功与否有着直接的关系，因而高效快速地从细菌细胞中分离纯化质粒具有重要意义[ 1 ] 。目前从细菌中提取质粒DNA多采用碱裂解法，该法操作简便
。 GhVP 基因的亚细胞定位 将构建好的 pBI121-GhVP∷GFP 载体用金粉包裹，把金粉包裹好的质粒加入基因枪 GDS-80 轰击洋葱内表皮细胞， 在激光共聚焦显微镜下观察。可以看出，空载体 pBI121-GFP 转化洋葱表皮后， 在整个洋葱细胞均能看到绿色荧光。pBI121-GhVP∷GFP 转化洋葱后，VP 蛋白定位于洋葱表皮细胞的细胞膜上，在玻片上加蔗糖，待质壁分离后，GhVP 蛋白在洋葱表皮细胞的细胞膜和细胞间质中均有分布（图3）。结果表明 GhVP 蛋白是 1 个跨膜蛋白， 与细胞
121 在文献"香蕉 Maasr1 基因 RNAi 植物表达载体的构建及鉴定.热带作物学 报， 2009 年， 30 (3) :333-337. "中公开过，公众可从中国农业科学院棉花研究所抗逆鉴定课 题组实验室获得。基因枪为美国 WEALTEC 的基因枪 GDS-80 。非抗虫棉花材料沪 749513 在文献"高频体细胞胚胎发生的优异棉花种质材料筛选.分子植物育种， 2012 ，10 (6) ，683-688. "中公开过，公众可从中国农业科学院棉花研究 所抗逆鉴定课题组实验室获得。 实施例
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pYC121载体
¥1200
