The Human STK11 Gene qBiomarker Somatic Mutation PCR Array allows the detection of 20 unique mutations in the human STK11 gene. STK11, a tumor suppressor gene and a serine/threonine kinase, regulates cell metabolism, homeostasis, and polarity via AMPK signaling. Previous scientific reports have shown a strong association between mutations in the STK11 gene and cancers of the breast, cervix, intestine, lung, pancreas, and skin. The STK11 mutations found in cancer samples include a roughly equal number of missense point mutations and truncations in the protein kinase domain. The DNA sequence mutation assays in the array detect the most frequent mutations in STK11 compiled from over 300 reported cancer sample sequences. Each mutation assay is bench-tested for the sensitive detection of a low percentage of mutation-containing DNA in a background of wild-type genomic DNA. The 96-well format of this array profiles the mutation status of 4 samples, while the 384-well array format profiles 16 samples, and each set of plates includes qPCR mastermix. Using real-time PCR, research studies can easily and reliably detect a panel of mutations in the STK11 gene with this array.