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文献和实验samples from the thermal cycler and immediately place on ice, with an ice pack on top of the samples, for 1 min. Turn off the power supply. Adjust comb if it has been pushed out of the gel during pre-heating. Load 4 µl of each sample per well. TIP
materials are required by law to supply the user with pertinent information on any hazards associated with their chemicals. This information is supplied in the form of M aterial S afety D ata S heets or MSDS. This information contains the chemical
Ex Ovo Electroporation of DNA Vectors into Pre-gastrulation Avian Embryos
the leads to the chamber. Position the cathode parallel to the anode, at a distance of ~3 mm. ii. Program the CUY-21TM power supply with the following pulse sequence: 5 V, 40 msec power-on, and 900 msec power-off, for a series
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