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文献和实验Lipid analysis－Week 3: GAS LIQUID CHROMATOGRAPHY
to evaporate. Repeat this over and over. Be careful not to bend the needle plunger as it is delicate. Place the syringe into the sample to be run (hexane for your first run). Remove air from the syringe by running the sample in and out of the syringe
on the dish, and rock the blot at 4℃ for 1 hour. This is the blocking step.6. Meanwhile, set up 2 spin columns:Use 1cc syringes, remove the cap and the plunger. Put in glass wool to fill the opening at the bottom, push down with the plunger. Put the syringe
Labelling Drosophila pupae with BrdU
just after pulling to the micro manipulator and connect it to a 1-ml syringe with air-filled plastic tubing (Fig. 2); any other kind of injection apparatus (oil-filled tubing, Pico-Injecter, etc.) will also be OK. 3. Break the tip of the needle with fine
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