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文献和实验Capillary Electrochromatography
In conventional liquid chromatography, the flow of eluent is generated by the application of a pressure gradient, which is usually provided by means of a high-pressure mechanical pump. However, under certain conditions, the mobile phase
Determination of Polyamines as Their Benzoylated Derivatives by HPLC
-water is the most widely used solvent system and most reported separations have been performed isocratically ( 1 – 5 , 7 , 8 , 10 – 12 ), obviating the need for a gradient maker or two pump system, although use of a gradient may enable the simultaneous
purification because of its low critical micelle concentration(0.0155% w/v) relative to CHAPS (0.492–0.615%w/v) and its cellular toxicity. In addition, in the presence ofTriton X-100, Wnt3A is incorporated into high molecularweight complexes (potentially
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