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上海希言科学仪器有限公司
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文献和实验CCMB buffer to make chemical competent cell
on ice 20 minutes Centrifuge again at 4C and resuspend in 20 ml of ice cold CCMB80 buffer. Incubate on ice for 20 minutes Aliquot to chilled screw top 2 ml vials or 50 μl into chilled microtiter plates Store at -80C indefinitely. Flash freezing
Labeling Muscle Actin with N-(1-pyrene)iodoacetamide
Day 0 and 1 Materials 1.0.5 mM ATP,0.2 mM CaCl2,2 mM Tris-HCl,pH 8.0 at 4℃,250 ml for day 0. 2.0.5 mM ATP,2 mM MgCl2,100 mM boric acid,pH 8.3 at 4℃,10 ml. 3.N-(1-pyrene)iodoacetamide (Molecular probes P-29,m.w.385) 4.50Ti tubes,small vial
preparedas described for Wnt3A CM in Section 3.1 ). 5. Incubate cells for 2 hours at 37°C in a humidified CO 2 incubator. 6. Aspirate all control and test wells and wash once with 100 μLof PBS. 7. Add 30 μL of Lysis Buffer, incubate 1–2 minutes
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