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文献和实验Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
-818.2009.03142.x (2009). 6. Hutson, M. S., & Ma, X. Plasma and cavitation dynamics during pulsed laser microsurgery in vivo . Physical review letters. 99, 158104 (2007). 7. Venugopalan, V., Guerra, A., 3rd, Nahen, K., & Vogel
. 5. Stand on ice for 5 to 15 min to allow sample to equilibrate and analyze for fluorescence immediately. top of page REAGENTS: Solution A: 0.1 % Triton X-100, 0.08N HCl, 0.15M NaCl Solution B: 6 ug/ml Acridine
suspension for 6 x 10 second bursts (with 10 second intervals between each burst) using a Misonix XL 2020 sonicator fitted with a microtip probe and set at power setting 5 (Figure 4 Left). Check the sonicated nuclei under a phase contrast microscope
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