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文献和实验lamp is OFF by default. To read absorbance in the UV range, turn the deuterium lamp on as follows after the machine has completed its calibration: Depress the function key until Fn5 is displayed. Press the mode key until d2on is displayed. Press enter
). Flicking the bottom of tubes ensured that all the DNA has been suitably resuspended. 2) The samples were then given a quick spin in a microcentrifuge and kept on ice prior to use. 3) On older models of the spectrometers, the deuterium lamp was turned
the filters under a heat lamp. Keep one filter aside and use it directly for the determination of total dpm in the sample. 3.Wash the other filter 3 times for 5min in 10ml 7.5% (w/v) Na2HPO4•12H2O for the removal of the unincorporated dNTPs
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