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上海希言科学仪器有限公司
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文献和实验(Cibacron Blue F3G-A coupledto Sepharose), ~100 mL packed into an empty column with100–200 mL of bed volume. 3. Binding Buffer: 1% (w/v) CHAPS, 150 mM KCl, and 20 mMTris-HCl, pH 7.5, sterile filtered. 4. Elution Buffer: 1% (w/v) CHAPS, 1.5 M KCl
. 7.TRITC,10% on celite (Research Organics). 8.Bio-Beads SM-2 in 0.7x15 column. Procedure (perform under reduced light) 1.Get 1-2 mg vinculin,stored in 2 mM Pipes,0.02% Azide,pH 7.6,in liquid nitrogen.Thaw quickly in warm water and chill in ice. 2.Mix
Obtaining DNA from Agarose Gels (Paper Slurry Method) 凝胶中回收DNA【University
tube. Pack with a 200 μl pipet tip and remove excess liquid. Repeat until paper column is approximately 3 mm high. Place inside a 1.5 mL tube to collect eluent. Procedure 1.Excise the DNA band from the surrounding gel with a clean razor blade
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