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文献和实验酶 (4)1.0M MgCl2 (5)2mg/ml DNA酶I,溶于50%甘油,75mM NaCl (6)Triton-X 100 (7)1M DTT (8)重悬液(PH8.0)50mM Tris-HCl(PH8.0)25%(W/V) 蔗糖 1mM EDTA 0.1%(W/V) 叠氮钠 10mM DTT(新鲜加入) (9)洗涤液I(PH 8.0)50mM Tris-HCl(PH8.0)0.5% Triton-X 100 100mM NaCl 1mM
at once, it is likely that they will all reach OD600 nm ~ 1.0 at different times. If desired, sodium azide (1M stock in water, diluted to a final concentration of 10 mM) can be added to a culture once it reaches an OD600 nm ~ 1.0. The culture can then be placed on ice
600 nm ~ 1.0 at different times. If desired, sodium azide (1M stock in water, diluted to a final concentration of 10 mM) can be added to a culture once it reaches an OD600 nm ~ 1.0. The culture can then be placed on ice until the others are ready.
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