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文献和实验Fluorescence Activated Cell Sorting (FACS)
the cells into a flask and forcing the cells to enter a small nozzle one at a time (figure 1). The cells travel down the nozzle which is vibrated at an optimal frequency to produce drops at fixed distance from the nozzle. As the cells flow down the stream
线粒体荧光探针大全:TMRM,Mitotracker,JC-1
morphology and organelle functioning. The uptake of most mitochondrion-selective dyes is dependent on the mitochondrial membrane potential; nonyl acridine orange and possibly our MitoTracker Green FM, MitoFluor Green and MitoFluor Red 589 probes are notable
miRNA microarrays(McManus Lab)(芯片)
supernatant. m) Wash the pellet with 500 μL 75% ice cold ethanol. Centrifuge at 16,000 xg for 10 minutes at 4°C. Discard the supernatant and air dry the pellet. 3. Labeling of RNA—this is best done just before hybridization
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