Whole Cell Extracts

 

<center> <p> <b><u><font color="#800000"><font>Whole Cell Extracts</font> </font> </u> </b></p> </center>

 

REFERENCE: Hoffman, G., Garrison, T. R., and Dohlman, H. G., Analysis of RGS proteins in Saccharomyces cerevisiae , Methods Enzymol. 344:617-631, 2002.
 

-Use sterile technique and sterile solutions in steps 1 to 3.-

1. Using a saturated starter culture, inoculate 25 to 30 ml of appropriate media in a 125 ml flask.