Eppendorf Reference® 2 单道固定量程移

快速定量检测动物源性食品中 β-受体激动剂残留

，在这里做统一说明：为了达到预期效果，形成湍流是关键。所谓吸打，不仅要吸入液体，在吸入液体之后还要吸入适量气体，所以移液器的量程一定要大于待吸入液体的量，在吸入液体或气体时，要缓慢释放移液器按钮，确保充分混匀。在吸入气体时，可以看见有大量气泡产生，让树脂随溶液湍流与样品充分、快速混合。如「 500 µL 甲醇吸打一次」的意思是：A. 将 1 mL 移液枪刻度调至 1 mL，连接 1 mL MicroSep® DPX tips；B. 将移液器控制按钮按至 1 档，将枪头插入至液面下（要保证容器内的液体

RT-PCR and PCR

Thermocycler can reach temperatures of 95 ℃. RT set-up 1. To a sterile 500 µl Eppendorf tube add in the following order: 2. Incubate 65 ûC for 5 min. 3. Cool slowly

Chromatin immunoprecipitation on native chromatin from cells and tissues

nuclei (as described the section titled Nuclei Preparation from Tissues and Cells) are re-suspended in 1mL of ice-cold MNase digestion buffer and placed on ice; Aliquot the suspension in two 1.5mL Eppendorf tubes (500µl of re-suspended nuclei