胆固醇 (Cholesterol) 是哺乳动物中主要的甾体类

Cholesterol is the major sterol in mammals and is makes up 20-25% of structural component of the plasma membrane. Plasma membranes are highly permeable to water but relatively impermeable to ions and protons. Cholesterol plays an important role in determining the fluidity and permeability characteristics of the membrane as well as the function of both the transporters and signaling proteins^[1][2]. Cholesterol is also an endogenous estrogen-related receptor α (ERRα) agonist^[3].

GT1-7 hypothalamic cells subjected to Cholesterol depletion in vitro produced 20-31% reductions in cellular Cholesterol content. All Cholesterol-depleted neuron-derived cells, exhibit decreased phosphorylation/activation of IRS-1 and AKT following stimulation by insulin, insulin-like growth factor-1, or the neurotrophins (NGF and BDNF). Reduction in cellular Cholesterol also results in increased basal autophagy and impairment of induction of autophagy by glucose deprivation^[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Insulin-deficient diabetes in mice can lead to a reduction in brain Cholesterol synthesis, which occurs through a change in expression of Cholesterol synthesis enzymes and their upstream regulators SREBP2 and SCAP1^[1].
The pool of Cholesterol in the whole animal is 2,200 mg/kg body weight. This is true for essentially all species from the mouse to the primate and indicates that the average concentration of Cholesterol in the whole animal is 2.2 mg/g fresh tissue^[2].
The basal metabolic rate in the mouse is 170 kilocalories (kcal)/day/kg, and the flow of Cholesterol from all peripheral organs to the liver is greater than 100 mg/day/kg^[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

386.65

C₂₇H₄₆O

57-88-5

胆固醇；胆甾醇

Room temperature in continental US; may vary elsewhere.

Ethanol : 25 mg/mL (64.66 mM; Need ultrasonic)

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用； 以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

• 1.

  请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline

  Solubility: ≥ 3.43 mg/mL (8.87 mM); Clear solution

• 2.

  请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)

  Solubility: ≥ 3.43 mg/mL (8.87 mM); Clear solution

• 3.

  请依序添加每种溶剂： 10% DMSO 90% corn oil

  Solubility: ≥ 3.43 mg/mL (8.87 mM); Clear solution

• 4.

  请依序添加每种溶剂： 10% EtOH 90% corn oil

  Solubility: ≥ 2.5 mg/mL (6.47 mM); Clear solution