- ￥1600 - 2650
- MultiSciences
- 15.63 pg/mL - 1000 pg/mL
- 浙江杭州
- EK304
- 2025年12月13日
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- 库存：
99
- 供应商：
联科生物
- 检测范围：
15.63 pg/mL - 1000 pg/mL
- 检测方法：
双抗夹心法
- 应用：
ELISA
- 适应物种：
Rat
- 标记物：
HRP
- 样本：
血清，血浆，细胞培养上清及其他生物学样本
- 规格：
96T/48T
| 规格： | 96T | 产品价格： | ￥2650.0 |
|---|---|---|---|
| 规格： | 48T | 产品价格： | ￥1600.0 |
描述
| 商品名 |
Rat IL-4 ELISA Kit (大鼠白细胞介素4 ELISA试剂盒) |
|---|---|
| 检测方法 |
双抗夹心法 |
| 精密度 |
板内变异系数：2.1% - 2.7%；板间变异系数：3.1% - 4.3% |
| 样本类型 |
血清，血浆，细胞培养上清及其他生物学样本 |
| 检测样本体积 |
20 μL |
| 灵敏度 |
0.26 pg/mL |
| 检测范围 |
15.63 pg/mL - 1000 pg/mL |
| 回收率 |
86% - 106% |
| 平均回收率 |
0.99 |
| 板式 |
96孔板，可拆 |
| 保存 |
试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。 |
| 运输条件 |
4℃蓝冰运输 |
| 组分 |
|
检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠IL-4抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的IL-4与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IL-4的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长5630 nm)测定吸光度值。
IL4 分子靶点信息概述
- 分子名：IL4, interleukin 4
- 基因家族：Interleukins
- 别名：BSF1; IL-4; BCGF1; BCGF-1; MGC79402
- 全称：B_cell stimulatory factor 1; lymphocyte stimulatory factor 1; B cell growth factor 1
IL4 分子靶点综述
IL-4是II型辅助T细胞(Th2细胞)分泌的细胞因子。IL-4是一种多效性细胞因子，在调节T、B淋巴细胞和其他类型细胞的增殖、分化、凋亡，促进以Th2细胞为特征的免疫应答过程中发挥重要作用。IL-4多重生物效应依赖于细胞类型和分化状态，研究表明其在介导过敏性疾病、自身免疫性疾病、感染性疾病、肿瘤等疾病的免疫反应中有多重作用，对肿瘤、自身免疫性疾病和感染性疾病等有治疗作用，并且IL-4对疫苗免疫应答具有调节作用。
大鼠 Rat Il4 分子靶点信息
- 分子名：Il4, interleukin 4
- 别称：
- B-cell growth factor 1
- B-cell IGG differentiation factor
- B-cell stimulatory factor 1
- BSF-1
- IL-4
- Il4e12
- interleukin-4
- lymphocyte stimulatory factor 1
- 基因序列：NCBI_Gene: 287287
- 蛋白序列：
- UniprotKB: A0A0G2JVY1
- UniprotKB: P20096
大鼠 Rat Il4 靶点分子功能（预测）
Predicted to enable cytokine activity. Involved in several processes, including positive regulation of biosynthetic process; positive regulation of leukocyte migration; and regulation of defense response. Located in extracellular space. Used to study anti-basement membrane glomerulonephritis; autoimmune thyroiditis; and pelvic inflammatory disease. Biomarker of several diseases, including cerebrovascular disease (multiple); interstitial lung disease (multiple); irritant dermatitis; primary biliary cholangitis; and rhinitis (multiple). Human ortholog(s) of this gene implicated in several diseases, including asthma (multiple); autoimmune disease (multiple); hepatitis B; hepatitis C; and pancreatic cancer (multiple). Orthologous to human IL4 (interleukin 4).
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