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- 4.69 pg/mL - 300 pg/mL
- 杭州
- EK382-96
- 2026年03月29日
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- 文献和实验
- 技术资料
- 库存：
99
- 供应商：
联科生物
- 检测范围：
4.69 pg/mL - 300 pg/mL
- 检测方法：
双抗夹心法
- 应用：
ELISA
- 适应物种：
Rat
- 标记物：
NoData
- 样本：
血清、血浆、细胞上清
- 规格：
96T/48T
| 规格： | 96T | 产品价格： | ￥2650.0 |
|---|---|---|---|
| 规格： | 48T | 产品价格： | ￥1600.0 |
| 背景介绍 | 肿瘤坏死因子α(TNF-α)也称为恶病质素和TNFSF1A，是一种脂肪因子，参与全身的炎症，同时也是刺激急性期反应的细胞因子之一。它主要由活化的巨噬细胞产生，也可由其它类型的细胞分泌，如CD4+淋巴细胞、NK细胞、中性粒细胞、肥大细胞、嗜酸性粒细胞和神经元等。它在免疫应答中具有多种调节功能，还可作为潜在的热原。TNF-α对刺激物(感染因子或组织受损)产生应答后，在全身循环，激活中性粒细胞，改变血管内皮细胞的特性，调控其它组织的代谢活性，以及通过诱导局部凝血作用展现肿瘤杀伤活性。TNF-α在关节组织和其它组织发生炎症的发病机制具有重要作用。最近，越来越多的信息表明TNF-α也参与了AIDS的发病机制。TNF-α的测定对于移植研究也非常有用。 |
| 检测原理 | 本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠TNF-α抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的TNF-α与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中TNF-α的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长570-630 nm)测定吸光度值。 |
| 组分 | 包被抗TNF-α单克隆抗体的96孔聚苯乙烯酶标板 大鼠TNF-α冻干标准品 TNF-α检测抗体 标准品稀释液 检测缓冲液(10×) 底物(TMB) 终止液 洗液(20×) 封板膜 |
| 分析类型 | 夹心ELISA |
| 样本类型 | 血清、血浆，细胞培养上清及其他生物学样本 |
| 板式 | 96孔板，可拆 |
| 保存 | 试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。 |
| 运输条件 | 4℃蓝冰运输 |
| 样本体积 | 10 μl |
| 灵敏度 | 0.43 pg/ml |
| 标准曲线范围 | 4.69-300 pg/ml |
| 回收率范围 | 82 %-116 % |
| 平均回收率 | 0.96 |
| 板间变异系数 | 6.1 %-8.4 % |
| 板内变异系数 | 4.4 %-6.3 % |
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- IF = 4.932 Bone marrow mesenchymal stem cells modified with heme oxygenase-1 alleviate rejection of donation after circulatory death liver transplantation by inhibiting dendritic cell maturation in rats. INTERNATIONAL IMMUNOPHARMACOLOGY (2022)
- IF = 4.932 Polydatin alleviates traumatic spinal cord injury by reducing microglial inflammation via regulation of iNOS and NLRP3 inflammasome pathway. INTERNATIONAL IMMUNOPHARMACOLOGY (2019)
- IF = 4.932 The protective effect of hypericin on postpartum depression rat model by inhibiting the NLRP3 inflammasome activation and regulating glucocorticoid metabolism. INTERNATIONAL IMMUNOPHARMACOLOGY (2022)
- IF = 4.932 Demethoxycurcumin mitigates inflammatory responses in lumbar disc herniation via MAPK and NF-κB pathways in vivo and in vitro. INTERNATIONAL IMMUNOPHARMACOLOGY (2022)
- IF = 4.932 Nicotinamide mononucleotide-elicited NAMPT signaling activation aggravated adjuvant-induced arthritis in rats by affecting peripheral immune cells differentiation. INTERNATIONAL IMMUNOPHARMACOLOGY (2021)
- IF = 4.927 Osthole Suppresses Knee Osteoarthritis Development by Enhancing Autophagy Activated via the AMPK/ULK1 Pathway. MOLECULES (2022)
- IF = 4.927 The Amomum tsao-ko Essential Oils Inhibited Inflammation and Apoptosis through p38/JNK MAPK Signaling Pathway and Alleviated Gentamicin-Induced Acute Kidney Injury. MOLECULES (2022)
- IF = 4.927 Inhibition of TLR4 Alleviates Heat Stroke-Induced Cardiomyocyte Injury by Down-Regulating Inflammation and Ferroptosis. MOLECULES (2023)
- IF = 4.91 AIFM1, negatively regulated by miR-145-5p, aggravates hypoxia-induced cardiomyocyte injury. Biomedical Journal (2021)
- IF = 4.868 Aspirin-Mediated Attenuation of Intervertebral Disc Degeneration by Ameliorating Reactive Oxygen Species In Vivo and In Vitro. Oxidative Medicine and Cellular Longevity (2019)
- IF = 4.845 Targeted elimination of intracellular reactive oxygen species using nanoparticle-like chitosan- superoxide dismutase conjugate for treatment of monoiodoacetate-induced osteoarthritis. INTERNATIONAL JOURNAL OF PHARMACEUTICS (2020)
- IF = 4.8 HMGA2 knockdown alleviates the progression of nonalcoholic fatty liver disease (NAFLD) by downregulating SNAI2 expression. CELLULAR SIGNALLING (2023)
- IF = 4.711 Cholinergic Elicitation Prevents Ventricular Remodeling via Alleviations of Myocardial Mitochondrial Injury Linked to Inflammation in Ischemia-Induced Chronic Heart Failure Rats. MEDIATORS OF INFLAMMATION (2021)
- IF = 4.711 Overexpression of TOLLIP Protects against Acute Kidney Injury after Paraquat Intoxication through Inhibiting NLRP3 Inflammasome Activation Modulated by Toll-Like Receptor 2/4 Signaling. MEDIATORS OF INFLAMMATION (2021)
- IF = 4.667 CD39/CD73/A2a Adenosine Metabolic Pathway: Targets for Moxibustion in Treating DSS-Induced Ulcerative Colitis. AMERICAN JOURNAL OF CHINESE MEDICINE (2021)
- IF = 4.627 Treatment with adipose tissue-derived mesenchymal stem cells exerts anti-diabetic effects, improves long-term complications, and attenuates inflammation in type 2 diabetic rats. Stem Cell Research & Therapy (2019)
- IF = 4.545 Puerarin improves the bone micro-environment to inhibit OVX-induced osteoporosis via modulating SCFAs released by the gut microbiota and repairing intestinal mucosal integrity. BIOMEDICINE & PHARMACOTHERAPY (2020)
- IF = 4.522 miR‐181a/b‐5p ameliorates inflammatory response in monocrotaline‐induced pulmonary arterial hypertension by targeting endocan. JOURNAL OF CELLULAR PHYSIOLOGY (2019)
- IF = 4.471 In Situ Forming Injectable Hydrogel For Encapsulation Of Nanoiguratimod And Sustained Release Of Therapeutics. International Journal of Nanomedicine (2019)
- IF = 4.451 Inhibition of Toll-Like Receptor 4 Signaling Pathway Accelerates the Repair of Avascular Necrosis of Femoral Epiphysis through Regulating Macrophage Polarization in Perthes Disease. Tissue Engineering and Regenerative Medicine (2023)
- IF = 4.451 The effect of Metapanax delavayi leaf extract on testosterone‐induced benign prostatic hyperplasia in rats. Journal of Functional Foods (2020)
- IF = 4.412 The Effect of Protein-Rich Extract from Bombyx Batryticatus against Glutamate-Damaged PC12 Cells Via Regulating γ-Aminobutyric Acid Signaling Pathway. MOLECULES (2020)
- IF = 4.374 Pirfenidone inhibits epithelial-mesenchymal transition and pulmonary fibrosis in the rat silicosis model. TOXICOLOGY LETTERS (2018)
- IF = 4.36 Effect of Shixiao San on inflammatory factors and pain in rats with endometriosis. JOURNAL OF ETHNOPHARMACOLOGY (2022)
- IF = 4.36 Tibetan medicine Ershiwuwei Lvxue Pill attenuates collagen-induced arthritis via inhibition of JAK2/STAT3 signaling pathway. JOURNAL OF ETHNOPHARMACOLOGY (2021)
- IF = 4.36 Yanning Syrup ameliorates the lipopolysaccharide-induced inflammation: Adjusting the gut microbiota, short-chain fatty acids, and the CD4+ T cell balance. JOURNAL OF ETHNOPHARMACOLOGY (2021)
- IF = 4.259 Xiaoyaosan exerts anxiolytic-like effects by down-regulating the TNF-α/JAK2-STAT3 pathway in the rat hippocampus. Scientific Reports (2017)
- IF = 4.225 Tetramethylpyrazine Ameliorates Lipopolysaccharide-Induced Sepsis in Rats via Protecting Blood–Brain Barrier, Impairing Inflammation and Nitrous Oxide Systems. Frontiers in Pharmacology (2020)
- IF = 4.225 The Anti-Depressive Effects of Hesperidin and the Relative Mechanisms Based on the NLRP3 Inflammatory Signaling Pathway. Frontiers in Pharmacology (2020)
- IF = 4.219 miR-181b-5p inhibits endothelial-mesenchymal transition in monocrotaline-induced pulmonary arterial hypertension by targeting endocan and TGFBR1. TOXICOLOGY AND APPLIED PHARMACOLOGY (2019)
- IF = 4.219 Salidroside alleviates diabetic neuropathic pain through regulation of the AMPK-NLRP3 inflammasome axis. TOXICOLOGY AND APPLIED PHARMACOLOGY (2021)
- IF = 4.197 Inhibition of miR-181a-5p reduces astrocyte and microglia activation and oxidative stress by activating SIRT1 in immature rats with epilepsy. LABORATORY INVESTIGATION (2020)
- IF = 4.196 Pharmacological Investigation of the Anti-Inflammation and Anti-Oxidation Activities of Diallyl Disulfide in a Rat Emphysema Model Induced by Cigarette Smoke Extract. Nutrients (2018)
- IF = 4.174 Down-regulation of long non-coding RNA XIST aggravates sepsis-induced lung injury by regulating miR-16-5p. Human Cell (2021)
- IF = 4.162 Prunella vulgaris L. Attenuates Experimental Autoimmune Thyroiditis by Inhibiting HMGB1/TLR9 Signaling. Drug Design Development and Therapy (2021)
- IF = 4.146 Mechanism of Incisional Pain: Novel Finding on Long Noncoding RNA XIST/miR-340-5p/RAB1A Axis:. ASN Neuro (2021)
- IF = 4.119 Silica dust exposure induces pulmonary fibrosis through autophagy signaling. ENVIRONMENTAL TOXICOLOGY (2021)
- IF = 4.119 The therapeutic effects of Prunella vulgaris against fluoride-induced oxidative damage by using the metabolomics method. ENVIRONMENTAL TOXICOLOGY (2021)
- IF = 4.092 MicroRNA-181b Inhibits Inflammatory Response and Reduces Myocardial Injury in Sepsis by Downregulating HMGB1. INFLAMMATION (2021)
- IF = 4.092 Protective Effects of 18β-Glycyrrhetinic Acid on Neonatal Rats with Hyperoxia Exposure. INFLAMMATION (2022)
- IF = 4.061 Therapeutic Potential of Liraglutide for Diabetes–Periodontitis Comorbidity: Killing Two Birds with One Stone. Journal of Diabetes Research (2022)
- IF = 4.061 San-Huang-Yi-Shen Capsule Ameliorates Diabetic Kidney Disease through Inducing PINK1/Parkin-Mediated Mitophagy and Inhibiting the Activation of NLRP3 Signaling Pathway.. Journal of Diabetes Research (2022)
- IF = 4.044 Gastric smooth muscle cells manifest an abnormal phenotype in Parkinson’s disease rats with gastric dysmotility. CELL AND TISSUE RESEARCH (2020)
- IF = 4.011 Pharmacokinetic-Pharmacodynamic Modeling for Coptisine Challenge of Inflammation in LPS-Stimulated Rats. Scientific Reports (2019)
- IF = 4.003 Long Non-coding RNAMALAT1 Knockdown Alleviates Cerebral Ischemia/Reperfusion Injury of Rats Through Regulating the miR-375/PDE4D Axis. Frontiers in Neurology (2021)
- IF = 3.943 Astragaloside IV alleviates PM2.5-induced lung injury in rats by modulating TLR4/MyD88/NF-κB signalling pathway. INTERNATIONAL IMMUNOPHARMACOLOGY (2020)
- IF = 3.943 LXA4 protects against hypoxic-ischemic damage in neonatal rats by reducing the inflammatory response via the IκB/NF-κB pathway. INTERNATIONAL IMMUNOPHARMACOLOGY (2020)
- IF = 3.943 Nervilifordin F alleviates intestinal ischemia/reperfusion-induced acute lung injury via inhibiting inflammasome and mTOR pathway. INTERNATIONAL IMMUNOPHARMACOLOGY (2020)
- IF = 3.935 Metabolomics and pharmacodynamic analysis reveal the therapeutic role of Prunella vulgaris oil on intrauterine adhesion rats. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS (2021)
- IF = 3.926 Protective effects of methylprednisolone–cyclophosphamide treatment on bleomycin-induced pulmonary fibrosis. CYTOKINE (2023)
- IF = 3.915 Resveratrol alleviates early brain injury following subarachnoid hemorrhage: possible involvement of the AMPK/SIRT1/autophagy signaling pathway. BIOLOGICAL CHEMISTRY (2018)
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大鼠肿瘤坏死因子α（TNF-α） 酶联免疫 分析(ELISA) 试剂 盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定大鼠血清，组织及相关液体样本中 肿瘤坏死因子α（TNF-α） 的含量。 实验原理 ： 本试剂盒应用双抗体夹心法测定 标本 中 大鼠 肿瘤坏死因子α（TNF- α） 水平。用纯化的 大鼠 肿瘤坏死因子α（TNF- α） 抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入 肿瘤坏死因子α
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Human/Mouse/rat Neural lineage Functional identification Kit（目录号 Sc028） 在神经祖细胞（NPc）的分离和扩增过程中，必须通过评估它们分化成多个神经谱系的能力，从而在功能上评估它们的多能性。本文介绍的试剂盒提供了一种快速简单的技术，可确认您的起始细胞群向星形胶质细胞、神经元和少突胶质细胞谱系分化的能力。 在实验过程的早期阶段验证多能性的能力： √ 定义起始干细胞群体 √ 减少实验中以及不同研究之间的可
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大鼠肿瘤坏死因子alpha试剂盒Rat TNF-alpha ELISA Kit/Rat TNF-α ELISA Kit (大鼠肿瘤坏死因子α ELISA试剂盒)
￥1600 - 2650
