- ￥1600 - 2650
- MultiSciences
- 31.25 pg/mL - 2000 pg/mL
- 70-EK380
- 浙江杭州
- 2026年04月12日
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- 文献和实验
- 技术资料
- 供应商：
联科生物
- 库存：
99
- 样本：
血清，血浆，细胞培养上清及其他生物学样本
- 标记物：
HRP
- 适应物种：
Rat
- 应用：
ELISA
- 检测方法：
双抗夹心法
- 检测范围：
31.25 pg/mL - 2000 pg/mL
- 规格：
48T/96T
| 规格： | 48T | 产品价格： | ￥1600.0 |
|---|---|---|---|
| 规格： | 96T | 产品价格： | ￥2650.0 |
描述
| 商品名 | Rat IFN-γ ELISA Kit (大鼠γ干扰素 ELISA试剂盒) |
|---|---|
| 检测方法 | 双抗夹心法 |
| 精密度 | 板内变异系数：4.1% - 6.0%；板间变异系数：5.4% - 6.6% |
| 样本类型 | 血清，血浆，细胞培养上清及其他生物学样本 |
| 检测样本体积 | 20 μL |
| 灵敏度 | 0.54 pg/mL |
| 检测范围 | 31.25 pg/mL - 2000 pg/mL |
| 回收率 | 87% - 112% |
| 平均回收率 | 0.99 |
| 板式 | 96孔板，可拆 |
| 保存 | 试剂盒未拆开，4℃保存。已拆开，标准品-20℃保存，其它4℃保存。 |
| 运输条件 | 4℃蓝冰运输 |
| 组分 |
|
检测原理：本试剂盒采用双抗体夹心酶联免疫吸附检测技术。特异性抗大鼠IFN-γ抗体预包被在高亲和力的酶标板上。酶标板孔中加入标准品、待测样本和生物素化的检测抗体，经过孵育，样本中存在的IFN-γ与固相抗体和检测抗体结合。洗涤去除未结合的物质后，加入辣根过氧化物酶标记的链霉亲和素(Streptavidin-HRP)。洗涤后，加入显色底物TMB，避光显色。颜色反应的深浅与样本中IFN-γ的浓度成正比。加入终止液终止反应，在450 nm波长(参考波长5630 nm)测定吸光度值。
标准曲线
分子信息
概述大鼠 IFNG, IFN-γ 靶点信息IFNG 分子靶点信息概述
- 分子名：IFNG, interferon gamma
- 基因家族：Interferons
IFNG 分子靶点综述
IFN-γ主要由Th1型细胞产生，属于II型细胞因子中干扰素家族中的一员，是一类分泌型糖蛋白，由143个氨基酸组成，相对分子质量是16942。IFN-γ主要由活化的T细胞和NK细胞产生，具有调节机体免疫状态，介导细胞免疫，加强受体介导的吞噬功能，增强巨噬细胞和中性粒细胞的杀伤活性，具有抗病毒感染及抗肿瘤等生物学活性。
大鼠 Rat Ifng 分子靶点信息
- 分子名：Ifng, interferon gamma
- 别称：
- If2f
- IFN-gamma
- IFNG2
- interferon 2f
- 基因序列：NCBI_Gene: 25712
- 蛋白序列：
- UniprotKB: A0A7R8C3J6
- UniprotKB: P01581
大鼠 Rat Ifng 靶点分子功能（预测）
Predicted to enable cytokine activity. Involved in several processes, including negative regulation of cell population proliferation; positive regulation of NMDA glutamate receptor activity; and positive regulation of macromolecule metabolic process. Located in several cellular components, including extracellular space; neuron projection; and perikaryon. Used to study several diseases, including Chagas disease; allergic conjunctivitis; allergic rhinitis; renal fibrosis; and ureteral obstruction. Biomarker of several diseases, including abdominal aortic aneurysm; autoimmune thyroiditis; pulpitis; tongue squamous cell carcinoma; and visual epilepsy. Human ortholog(s) of this gene implicated in several diseases, including ataxia telangiectasia; autoimmune disease (multiple); eczema herpeticum; factor VIII deficiency; and leukemia (multiple). Orthologous to human IFNG (interferon gamma).
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- IF = 8.786 Transcriptomic analysis of graft liver provides insight into the immune response of rat liver transplantation.. Frontiers in Immunology (2022)
- IF = 7.446 Discovery and In Vivo Anti-inflammatory Activity Evaluation of a Novel Non-peptidyl Non-covalent Cathepsin C Inhibitor. JOURNAL OF MEDICINAL CHEMISTRY (2021)
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- IF = 6.575 Integrating widely targeted lipidomics and transcriptomics unravels aberrant lipid metabolism and identifies potential biomarkers of food allergies in rats. MOLECULAR NUTRITION & FOOD RESEARCH (2023)
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- IF = 6.058 M2 macrophages mediate fibrotic scar formation in the early stages after cerebral ischemia in rats. Neural Regeneration Research (2023)
- IF = 5.89 The Therapeutic Efficacy of Adipose Tissue-Derived Mesenchymal Stem Cell Conditioned Medium on Experimental Colitis Was Improved by the Serum From Colitis Rats. Frontiers in Bioengineering and Biotechnology (2021)
- IF = 5.811 An Integrated Gut Microbiota and Network Pharmacology Study on Fuzi-Lizhong Pill for Treating Diarrhea-Predominant Irritable Bowel Syndrome.. Frontiers in Pharmacology (2021)
- IF = 5.385 Activation of the Aryl Hydrocarbon Receptor Ameliorates Acute Rejection of Rat Liver Transplantation by Regulating Treg Proliferation and PD-1 Expression. TRANSPLANTATION (2022)
- IF = 5.293 Gut Microbiota Is Involved in Alcohol-Induced Osteoporosis in Young and Old Rats Through Immune Regulation. Frontiers in Cellular and Infection Microbiology (2021)
- IF = 5.195 Yi-Qi-Jian-Pi formula ameliorates immune function in acute-on-chronic liver failure by upregulating autophagy and mitochondrial biogenesis in CD8+ T lymphocytes. JOURNAL OF ETHNOPHARMACOLOGY (2023)
- IF = 5.194 Safety considerations of chloroquine in the treatment of patients with diabetes and COVID-19. CHEMICO-BIOLOGICAL INTERACTIONS (2022)
- IF = 5.062 Multi-unit pellet drug delivery system of Danggui Decoction extracts for chemoprevention of IBD-associated colorectal cancer in rats. JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY (2022)
- IF = 4.932 Bone marrow mesenchymal stem cells modified with heme oxygenase-1 alleviate rejection of donation after circulatory death liver transplantation by inhibiting dendritic cell maturation in rats. INTERNATIONAL IMMUNOPHARMACOLOGY (2022)
- IF = 4.493 Targeting Ferroptosis Attenuates Inflammation, Fibrosis, and Mast Cell Activation in Chronic Prostatitis. Journal of Immunology Research (2022)
- IF = 4.36 Yanning Syrup ameliorates the lipopolysaccharide-induced inflammation: Adjusting the gut microbiota, short-chain fatty acids, and the CD4+ T cell balance. JOURNAL OF ETHNOPHARMACOLOGY (2021)
- IF = 3.943 Hepatocyte growth factor mediates a novel form of hepatic stem/progenitor cell-induced tolerance in a rat xenogeneic liver rejection model. INTERNATIONAL IMMUNOPHARMACOLOGY (2020)
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- IF = 3.69 Ethyl acetate extract of Tibetan medicine Rhamnella gilgitica ameliorated type II collagen-induced arthritis in rats via regulating JAK-STAT signaling pathway. JOURNAL OF ETHNOPHARMACOLOGY (2020)
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- IF = 3.337 A Hybrid Glioma Tumor Cell Lysate Immunotherapy Vaccine Demonstrates Good Clinical Efficacy in the Rat Model. OncoTargets and Therapy (2020)
- IF = 3.061 The effects of Saccharomyces boulardii on rat colonic hypermotility induced by repeated water avoidance stress and the potential mechanism. PeerJ (2022)
- IF = 3.004 A metabonomics and lipidomics based network pharmacology study of qi-tonifying effects of honey-processed Astragalus on spleen qi deficiency rats. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES (2020)
- IF = 2.936 Chronic glucocorticoid treatment induced circadian clock disorder leads to lipid metabolism and gut microbiota alterations in rats. LIFE SCIENCES (2017)
- IF = 2.152 Effects of Mongolian Warm Acupuncture on iNOS/NO and Inflammatory Cytokines in the Hippocampus of Chronic Fatigue Rats. Frontiers in Integrative Neuroscience (2020)
- IF = 1.984 Investigation of Efficacy Enhancing and Toxicity Reducing Mechanism of Combination of Aconiti Lateralis Radix Praeparata and Paeoniae Radix Alba in Adjuvant-Induced Arthritis Rats by Metabolomics. Evidence-based Complementary and Alternative Medicine (2019)
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- IF = 1.786 Zinc-enriched probiotics enhanced growth performance, antioxidant status, immune function, gene expression, and morphological characteristics of Wistar rats raised under high ambient temperature. 3 Biotech (2019)
- IF = 1.291 Increased expression and functionality of the gap junction in peripheral blood lymphocytes is associated with hypertension-mediated inflammation in spontaneously hypertensive rats. CELLULAR & MOLECULAR BIOLOGY LETTERS (2018)
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- IF = 0.636 T Lymphocyte Subsets and Cytokines in Rats Transplanted with Adipose-Derived Mesenchymal Stem Cells and Acellular Nerve for Repairing the Nerve Defects. Journal of Korean Neurosurgical Society (2015)
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