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Anti-rat IgG (H+L), (Alexa Flu

or 647 Conjugate)__®
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  • 询价
  • Cell Signaling Technology已认证
  • 4418
  • USA
  • 2025年09月12日
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    品牌商
    13钻石会员
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体英文名

      Anti-rat IgG (H+L), (Alexa Fluor 647 Conjugate)__®

    • 库存

      大量

    • 供应商

      CST

    • 保质期

      详见说明书

    • 级别

      详见MSDS文件

    • 标记物

      Alexa Fluor 647

    • 是否单克隆

      0

    • 保存条件

      -20°c

    • 规格

      250 ul/carrier free & custom formulation / quantity

    规格:产品价格:¥请询价
    规格:250 ul产品价格:¥请询价
    规格:carrier free & custom formulation / quantity产品价格:¥请询价

    Secondary Antibodies

    Anti-rat IgG (H+L), (Alexa Fluor® 647 Conjugate) #4418

    Specificity / Sensitivity

    IgG is prepared from goat antibodies that have been adsorbed against mouse IgG, mouse serum and human serum.

    Flow Cytometry

    Flow Cytometry

    Flow cytometric analysis of untreated HeLa cells using HSP70 (6B3) Rat mAb #4873 detected with Anti-rat IgG (H+L), (Alexa Fluor® 647 Conjugate) (green) and compared to a nonspecific negative control antibody (red).

    IF-IC

    IF-IC

    Confocal immunofluorescent analysis of HeLa cells using ORC2 (3G6) Rat mAb #4736 detected with Anti-rat IgG (H+L), (Alexa Fluor® 647 Conjugate) (blue) and COX IV (3E11) Rabbit mAb #4850 detected with Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 (green). Actin filaments have been labeled with DY-554 phalloidin (red).

    Description

    Anti-rat IgG (H+L) was conjugated to Alexa Fluor® 647 fluorescent dye under optimal conditions and formulated at 2 mg/ml.

    Directions for Use

    The optimal dilution of the secondary antibody should be determined for each application. However, a final dilution of 1:1000 should yield acceptable results for most immunofluorescent applications.

    Applications

    This product has been optimized for use as a secondary antibody in immunofluorescent applications.

    Background

    Fluorescent anti-species IgG conjugates are ideal for flow cytometry and immunofluorescence. Cell Signaling Technology’s strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.

    Application References

    Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

    Companion Products

    The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer (whether the buyer is an academic or for-profit entity), for immunocytochemistry, high content screening (HCS) analysis, or flow cytometry applications. The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) resale, whether or not such product or its components are resold for use in research; or for any other commercial purpose. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.


    For Research Use Only. Not For Use In Diagnostic Procedures.

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    图标文献和实验
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