- 询价
- Cell Signaling Technology
- 9654
- USA
- 2025年09月10日
- W
- H,M,R,Mk
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb (Biotinylated)
- 抗原：
synthetic peptide corresponding to amino-terminal residues adjacent to Asp175 of human caspase-3
- 应用范围：
W
- 适应物种：
H,M,R,Mk
- 供应商：
CST
- 保质期：
详见说明书
- 库存：
大量
- 级别：
详见MSDS文件
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H M R Mk | Endogenous | 17, 19 | Rabbit IgG |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb (Biotinylated) detects endogenous levels of the large fragment (17/19 kDa) of activated caspase-3 resulting from cleavage adjacent to Asp175. This antibody does not recognize full-length caspase-3 or other cleaved caspases. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues adjacent to Asp175 of human caspase-3. Western Blotting
Western blot analysis of extracts from HeLa cells, untreated or treated with Staurosporine #9953 (1 μM, 3 hrs), using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb (Biotinylated) and detected with Streptavidin-HRP #3999. |
| Description | This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb #9664. |
| Background | Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 position (2). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
Rabbit monoclonal antibody is produced under license (granting certain rights including those under U. S. Patents No. 5,675,063 and 7,429,487) from Epitomics, Inc. For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验，EDTA 缓冲液适用于多数磷酸化酪氨酸特异性抗体，而柠檬酸盐缓冲液适用于其他多数抗体。每天都需制备新鲜的 1X 溶液。抗体：hospho-HER3/ErbB3 (Tyr1289) (D1B5) Rabbit mAb #2842样本：蜡包埋人类肺部肿瘤抗原修复缓冲液：柠檬酸盐缓冲液（左）或者 EDTA 缓冲液（右）煮沸装置载玻片在所推荐的缓冲液中加热煮沸一段时间后，才可引起抗原修复。该步骤通常在微波炉或高压锅中进行。虽然，一些研究人员也使用水浴锅，但是，因为该步骤中所选择的装置对染色存在积极或消极的影响
ELISPOT (Enzyme-linked ImmunoSPOT)
Mouse Cytokine ELISPOT Set Catalog No. Capture mAb Detection mAb IFN-γ 88-7384 AN-18 R4-6A2 IL-1α 88-7813 ALF-161 Rabbit
Immunohistochemisty-Enzymatic Protocol
Overview R&D Systems provides monoclonal, polyclonal and biotinylated antibodies for immunohistochemical use. The following protocol has been developed and optimized by R&D Systems'' Immunohistochemical Laboratory. R&D Systems
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