Stat3 (124H6) Mouse mAb

价格询价

品牌Cell Signaling Technology
货号
9139
产地USA
更新日期2026年05月28日

应用范围W, IP, IHC-P, IF-IC, F, ChIP
适应物种H,M,R,Mk

美国CST中国公司(丁香通商铺)

品牌商

13 年钻石会员

详细信息
询价记录
文献和实验
技术资料

抗体英文名：
Stat3 (124H6) Mouse mAb
抗原：
synthetic peptide corresponding to the sequence of mouse Stat3
应用范围：
W, IP, IHC-P, IF-IC, F, ChIP
级别：
详见MSDS文件
适应物种：
H,M,R,Mk
供应商：
CST
保质期：
详见说明书
库存：
大量
是否单克隆：
1
保存条件：
-20°c
规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity

规格：		产品价格：	￥请询价
规格：	100 ul (10 western blots)	产品价格：	￥请询价
规格：	carrier free & custom formulation / quantity	产品价格：	￥请询价

pathway more info application references datasheet PDF MSDS PDF protocols

Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry ChIP=Chromatin IP
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Applications	Reactivity	Sensitivity	MW (kDa)	Isotype
W IP IHC-P IF-IC F ChIP	H M R Mk	Endogenous	79, 86	Mouse IgG2a

Protocols	9139: ChIP Agarose , ChIP Magnetic , Flow , IHC / Paraffin , Immunofluorescence* , Immunoprecipitation , Western Blotting * Product-specific protocol.
Specificity / Sensitivity	Stat3 (124H6) Mouse mAb detects endogenous levels of total Stat3 protein.
Source / Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of mouse Stat3. Western Blotting Western blot analysis of extracts from HeLa, NIH/3T3, PC12 and COS cells, using Stat3 (124H6) Mouse mAb. IHC-P (paraffin) Immunohistochemical analysis of paraffin-embedded human breast carcinoma (left), showing nuclear and cytoplasmic staining, and human lung carcinoma (right), showing cytoplasmic staining, using Stat3 (124H6) Mouse mAb. Flow Cytometry Flow cytometric analysis of Hela cells, untreated (blue) or IFN-alpha-treated (green), using Stat3 (124H6) Mouse mAb compared with a nonspecific negative control antibody (red). IF-IC Confocal immunofluorescent analysis of HeLa cells either serum-starved (left) or IFNalpha-treated (right) and labeled with Stat3 (124H6) Mouse mAb (green). Chromatin IP Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10⁶ Hep G2 cells starved overnight and treated with IL-6 (100 ng/ml) for 30 minutes, and either 10 μl of Stat3 (124H6) Mouse mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP^® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human IRF-1 promoter primers, SimpleChIP^® Human c-Fos Promoter Primers #4663, and SimpleChIP^® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
Background	The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8). Heim, M.H. (2001) J Recept Signal Transduct Res 19, 75-120. Takeda, K. et al. (1997) Proc Natl Acad Sci U S A 94, 3801-4. Catlett-Falcone, R. et al. (1999) Immunity 10, 105-15. Garcia, R. and Jove, R. (1998) J Biomed Sci 5, 79-85. Bromberg, J.F. et al. (1999) Cell 98, 295-303. Darnell, J.E. et al. (1994) Science 264, 1415-21. Ihle, J.N. (1995) Nature 377, 591-4. Wen, Z. et al. (1995) Cell 82, 241-50. Yokogami, K. et al. (2000) Curr Biol 10, 47-50. Biethahn, S. et al. (1999) Exp Hematol 27, 885-94.
Application References	Yao, Z. et al. (2010) Proc Natl Acad Sci U S A 107, 15535-40. Applications: Western Blotting Matsuyama, H. et al. (2011) Blood 118, 6881-92. Applications: ChIP Western Blotting Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
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