- 询价
- Cell Signaling Technology
- 9138
- USA
- 2025年12月27日
- W, IP, F
- H,M,R,B
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Phospho-Stat3 (Tyr705) (3E2) Mouse mAb
- 抗原：
synthetic phosphopeptide corresponding to residues surrounding Tyr705 of mouse Stat3
- 应用范围：
W, IP, F
- 级别：
详见MSDS文件
- 保质期：
详见说明书
- 库存：
大量
- 供应商：
CST
- 适应物种：
H,M,R,B
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/300 ul (30 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | 300 ul (30 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP F | H M (R) (B) | Endogenous | 79, 86 | Mouse IgG1 |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-Stat3 (Tyr705) (3E2) Mouse mAb detects endogenous levels of Stat3 only when phosphorylated at tyrosine 705. The antibody does not significantly cross-react with other members of the Stat family. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr705 of mouse Stat3. Western Blotting
Western blot analysis of extracts from HeLa cells, untreated or IFN-alpha-treated (100 ng/ml), using Phospho-Stat3 (Tyr705) (3E2) Mouse mAb. Flow Cytometry
Flow cytometric analysis of NIH/3T3 cells, untreated (red) or IFN-alpha treated (blue), using Phospho-Stat3 (Tyr705) (3E2) Mouse mAb. |
| Background | The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验。所以，我们建议使用微波炉或高压锅加热煮沸以达到最佳抗原修复效果。抗体：Phospho–Stat3 (Tyr705)(D3A7) XP. Rabbit mAb #9145样本：石蜡包埋人类肺部肿瘤样本煮沸设备：浴锅 (左)，微波炉 (中)，高压锅 (右)免疫染色—封闭在 IHC–P 中，我们建议在室温下在含有 Tween20 的 TBST 缓冲液和 5% 正常山羊血清 (NGS) 中封闭样品，时长 1 小时，以防止非特异性背景染色。在进行 IHC–F 时，在含有 0.3% Triton. X–100 的 1X
稀释液的效果更好。4. 匹配的二抗和显色系统——「配一配和滴一滴」使用多聚 HRP（Poly-HRP）的二抗，即在二抗上连接大量 HRP，以充分放大信号；同时，配合优质的色原产品，提升灵敏度，增强信号。注：在人肺癌标本中使用 Sox2(D6D9) XP® RabbitmAb#3579 检测 Sox2 的表达，使用基于多聚-HRP 的 SignalStain® BoostIHCDetectionReagent 的检测灵敏度更高。注：DAB 色原比较：在人乳腺癌组织中使用 p-Stat3(Tyr705)(D
：使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。（图 A）使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit，抗体 1:100 稀释；(图 B) 采用普通免疫组化试剂盒，抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注：使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK （Erk1/2）Rabbit mAb 对正常小鼠心脏组织进行免疫
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