- 询价
- Cell Signaling Technology
- 4850
- USA
- 2026年01月11日
- western blot,免疫沉淀（IP）,免疫组化（IHC）,免疫组化（IHC）,免疫荧光（IF）,流式细胞（Flow Cyt）
- 兔
- 人,大鼠,驴,斑马鱼,细菌,猪
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
COX IV (3E11) Rabbit mAb
- 抗原：
/
- 应用范围：
western blot,免疫沉淀（IP）,免疫组化（IHC）,免疫组化（IHC）,免疫荧光（IF）,流式细胞（Flow Cyt）
- 宿主：
兔
- 抗原来源：
/
- 保质期：
详见说明书
- 适应物种：
人,大鼠,驴,斑马鱼,细菌,猪
- 供应商：
CST
- 库存：
大量
- 级别：
详见MSDS文件
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
40 ul (4 western blots)/100 ul (10 western blots)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> /40 ul (4 western blots)/100 ul (10 western blots)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a>
| 规格： | 产品价格： | ￥面议 | |
|---|---|---|---|
| 规格： | 40 ul (4 western blots) | 产品价格： | ￥请询价 |
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
| 规格： | 40 ul (4 western blots) | 产品价格： | ￥请询价 |
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
Product Pathways - Cytoskeletal Signaling
COX IV (3E11) Rabbit mAb #4850
PhosphoSitePlus ® protein, site, and accession data: COX4I1
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IHC-P IHC-F IF-IC F | H R Mk Z B Pg | Endogenous | 17 | Rabbit IgG |
Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human R=Rat Mk=Monkey Z=Zebrafish B=Bovine Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 4850:
- Flow , IHC / Frozen , IHC / Paraffin , Immunofluorescence , Immunoprecipitation , Western Blotting
Specificity / Sensitivity
COX IV (3E11) Rabbit mAb detects endogenous levels of total COX IV protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys29 of human COX IV. Antibodies were purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from HeLa, Jurkat and COS cell lines, using COX IV (3E11) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, showing staining of the mitochondria, using COX IV (3E11) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using COX IV (3E11) Rabbit mAb in the presence of control peptide (left) or Cox IV Blocking Peptide #1034 (right).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded H1650 xenograft, using COX IV Rabbit mAb. Note specific staining of human cancer cells.
IHC-F (frozen)
Immunohistochemical analysis of frozen H1650 xenograft, using Cox IV (3E11) Rabbit mAb.
Flow Cytometry
Flow cytometric analysis of HeLa cells, using COX IV (3E11) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).
IF-IC
Confocal immunofluorescent analysis of HeLa cells labeled with COX IV (3E11) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Background
Cytochrome c oxidase (COX) is a hetero-oligomeric enzyme consisting of 13 subunits localized to the inner mitochondrial membrane (1-3). It is the terminal enzyme complex in the respiratory chain, catalyzing the reduction of molecular oxygen to water coupled to the translocation of protons across the mitochondrial inner membrane to drive ATP synthesis. The 3 largest subunits forming the catalytic core are encoded by mitochondrial DNA while the other smaller subunits, including COX IV, are nuclear-encoded. Research studies have shown that deficiency in COX activity correlates with a number of human diseases (4). The COX IV antibody can be used effectively as a mitochondrial loading control in cell-based research assays.
- Ostermeier, C. et al. (1996) Curr. Opin. Struct. Biol. 6, 460-466.
- Capaldi, R.A. et al. (1983) Biochim. Biophys. Acta 726, 135-148.
- Kadenbach, B. et al. (2000) Free Radic. Biol. Med. 29, 211-221.
- Barrientos, A. et al. (2002) Gene 286, 53-63.
Application References
- Aschrafi, A. et al. (2008) J Neurosci 28, 12581-90. Applications: Western Blotting
- Obexer, P. et al. (2009) J Biol Chem 284, 30933-40. Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
Companion Products
- 4844 COX IV Antibody
- 7071 Phototope® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 4967 β-Actin Antibody
- 4272 Cytochrome c Antibody
- 1034 COX IV Blocking Peptide
- 8114 SignalStain® Boost IHC Detection Reagent (HRP, Rabbit)
- 5425 Normal Goat Serum
- 8112 SignalStain® Antibody Diluent
- 9997 Tris Buffered Saline with Tween 20 (TBST-10X)
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
For Research Use Only. Not For Use In Diagnostic Procedures.
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