- 询价
- Cell Signaling Technology
- 4814
- USA
- 2026年05月27日
- western blot,免疫沉淀（IP）,免疫组化（IHC）,免疫荧光（IF）,流式细胞（Flow Cyt）
- 小鼠
- 人,小鼠,大鼠,驴,细菌,猪,豚鼠
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- 文献和实验
- 技术资料
- 抗体英文名：
IκBα (L35A5) Mouse mAb (Amino-terminal Antigen)
- 抗原：
/
- 应用范围：
western blot,免疫沉淀（IP）,免疫组化（IHC）,免疫荧光（IF）,流式细胞（Flow Cyt）
- 宿主：
小鼠
- 抗原来源：
/
- 保质期：
详见说明书
- 供应商：
CST
- 适应物种：
人,小鼠,大鼠,驴,细菌,猪,豚鼠
- 级别：
详见MSDS文件
- 库存：
大量
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
40 ul (4 western blots)/100 ul (10 western blots)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> /40 ul (4 western blots)/100 ul (10 western blots)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a>
| 规格： | 产品价格： | ￥面议 | |
|---|---|---|---|
| 规格： | 40 ul (4 western blots) | 产品价格： | ￥请询价 |
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
| 规格： | 40 ul (4 western blots) | 产品价格： | ￥请询价 |
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
Product Pathways - NF-kB Signaling
IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) #4814
PhosphoSitePlus ® protein, site, and accession data: IkB-alpha
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IHC-P IF-IC F | H M R Mk B Pg GP | Endogenous | 39 | Mouse IgG1 |
Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey B=Bovine Pg=Pig GP=Guinea Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 4814:
- Flow , IHC / Paraffin , Immunofluorescence , Immunoprecipitation , Western Blotting
Specificity / Sensitivity
IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) detects endogenous levels of total IκBα protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a GST-IκBα fusion protein corresponding the amino-terminus of human IκBα.
Western Blotting
Western blot analysis of extracts from THP-1 cells, differentiated with TPA (#9905, 80 nM for 24h) and treated with 1 μg/ml LPS for the indicated times, using Phospho-IκBα (Ser32/36) (5A5) Mouse mAb #9246 (upper) and IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) (lower).
Western Blotting
Western blot analysis of extracts from HeLa, NIH/3T3 and PC12 cells, using IkBα (L35A5) Mouse mAb (Amino-terminal Antigen).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human leiomyoma, using IκBα (L35A5) Mouse mAb (Amino-terminal Antigen).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using IκBα (L35A5) Mouse mAb (Amino-terminal Antigen).
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human renal adenocarcinoma, using IκBα (L35A5) Mouse mAb (Amino-terminal Antigen).
Flow Cytometry
Flow cytometric analysis of HeLa cells, using IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) (blue) compared to a nonspecifc negative control antibody (red).
IF-IC
Confocal immunofluorescent analysis of HeLa cells, untreated (left), or TNF-α-treated (#8902, 10 ng/ml for 15 min, right) using IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Background
The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8).
- Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6.
- Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70.
- Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8.
- Brown, K. et al. (1995) Science 267, 1485-8.
- Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18.
- Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
- Chen, Z.J. et al. (1996) Cell 84, 853-62.
- Karin, M. and Ben-Neriah, Y. (2000) Annu Rev Immunol 18, 621-63.
Application References
- Cui, W. et al. (2007) Proc Natl Acad Sci U S A 104, 14436-41. Applications: Western Blotting
- Shimada, M. et al. (2008) Development 135, 2001-11. Applications: Western Blotting
- Harrison, L.M. et al. (2008) Infect Immun , . Applications: Western Blotting
- Mabilleau, G. and Sabokbar, A. (2009) PLoS One 4, e4173. Applications: Western Blotting
- Kwong, C. et al. (2011) J Immunol 186, 1781-9. Applications: Western Blotting
- Barisic, S. et al. (2010) Biochem Pharmacol 80, 439-47. Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
Companion Products
- 4812 IκBα (44D4) Rabbit mAb
- 9242 IκBα Antibody
- 9247 IκBα (112B2) Mouse mAb (Carboxy-terminal Antigen)
- 2859 Phospho-IκBα (Ser32) (14D4) Rabbit mAb
- 9246 Phospho-IκBα (Ser32/36) (5A5) Mouse mAb
- 9248 IκBβ Antibody
- 4921 Phospho-IκBβ (Thr19/Ser23) Antibody (Human Specific)
- 4924 Phospho-IκBε (Ser18/22) Antibody
- 7072 Phototope® -HRP Western Blot Detection System, Anti-mouse IgG, HRP-linked Antibody
- 7076 Anti-mouse IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 8902 Human Tumor Necrosis Factor-α (hTNF-α)
- 8125 SignalStain® Boost IHC Detection Reagent (HRP, Mouse)
For Research Use Only. Not For Use In Diagnostic Procedures.
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文献和实验Antigen Design Sera Purification Tech Sheet
-terminal amino acid and vice versa for C-terminal sequences. Internal sequences can be coupled at either end. Another consideration for internal sequences is to acetlyate or amidate the unconjugated end as the sequence in the native protein molecule
T-Cell Activation Using mAb to CD3
One of the most common ways to assess T cell activation is to measure T cell proliferation upon in vitro stimulation of T cells via antigen or agonistic antibodies to TCR. This protocol is written as a starting point for examining in vitro proliferation of mouse
Antigen Design Sera Purification Tech Sheet
A factor that is often over-looked when designing a synthetic peptide is the method of coupling the peptide to the carrier protein. For example, N-terminal sequences should be coupled through the C-terminal amino acid and vice versa for C
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