- 询价
- Cell Signaling Technology
- 4223
- USA
- 2025年12月09日
- W, IP
- H
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Bcl-2 (D55G8) Rabbit mAb (Human Specific)
- 抗原：
synthetic peptide corresponding to residues surrounding Gly47 of human Bcl-2
- 应用范围：
W, IP
- 供应商：
CST
- 级别：
详见MSDS文件
- 库存：
大量
- 适应物种：
H
- 保质期：
详见说明书
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation
Reactivity Key: H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP | H | Endogenous | 26 | Rabbit IgG |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Bcl-2 (D55G8) Rabbit mAb (Human Specific) detects endogenous levels of total Bcl-2 protein. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly47 of human Bcl-2. Western Blotting
Western blot analysis of extracts from various cell lines using Bcl-2 (D55G8) Rabbit mAb (Human Specific). |
| Background | Bcl-2 exerts a survival function in response to a wide range of apoptotic stimuli through inhibition of mitochondrial cytochrome c release (1). It has been implicated in modulating mitochondrial calcium homeostasis and proton flux (2). Several phosphorylation sites have been identified within Bcl-2 including Thr56, Ser70, Thr74 and Ser87 (3). It has been suggested that these phosphorylation sites may be targets of the ASK1/MKK7/JNK1 pathway, and that phosphorylation of Bcl-2 may be a marker for mitotic events (4,5). Mutation of Bcl-2 at Thr56 or Ser87 inhibits its anti-apoptotic activity during glucocorticoid-induced apoptosis of T lymphocytes (6). Interleukin 3 and JNK-induced Bcl-2 phosphorylation at Ser70 may be required for its enhanced anti-apoptotic functions (7).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Dual- and Triple-Color Fluorospot
antibodies (mAbs) to cytokines: Mouse mAb to human IFN-γ; mAb 1-D1K for coating and FITC-labeled mAb 7-B6-1 for detection. Mouse mAb to human IL-2; mAbs IL2-I/249 for coating and biotinylated mAb IL2-II for detection. If a third cytokine is included
ELISPOT (Enzyme-linked ImmunoSPOT)
-specific responses, applicable to a wide range of areas of immunology research, including cancer, transplantation, infectious disease, and vaccine development. Human IL-17
Flow Cytometric Analysis Of Bcl Family members
was analyzed using CELLQUEST software. Live cells were gated on the basis of forward and side scatter and a minimum of 10,000 events were analyzed. Recipes The primary antibodies used were mouse monoclonal AB against Bcl-2 (Dako, USA), rabbit polyclonal
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