- 询价
- Cell Signaling Technology
- 3098
- USA
- 2025年12月25日
- W
- H
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- 详细信息
- 技术资料
- 抗体英文名：
Phospho-c-Abl (Tyr89) (61A6) Rabbit mAb
- 抗原：
synthetic phosphopeptide corresponding to residues surrounding Tyr89 of human c-Abl
- 应用范围：
W
- 供应商：
CST
- 库存：
大量
- 级别：
详见MSDS文件
- 适应物种：
H
- 保质期：
详见说明书
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
40 ul (4 western blots)/100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 40 ul (4 western blots) | 产品价格： | ￥请询价 |
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H | Endogenous | 135 (c-Abl); 210 (Bcr-Abl) | Rabbit IgG |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-c-Abl (Tyr89) (61A6) Rabbit mAb detects endogenous levels of c-Abl only when phosphorylated at Tyr89. This antibody may cross-react with other tyrosine-phosphorylated proteins. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr89 of human c-Abl. Western Blotting
Western blot analysis of extract from CML-T1 leukemic cells with Phospho-c-Abl (Tyr89) (61A6) Rabbit mAb (upper and middle) or c-Abl Antibody #2862 (lower). The phospho-specificity of this rabbit mAb was verified by treating the membrane with calf intestinal phosphatase (CIP) (middle and lower) before antibody probing. |
| Background | The c-Abl proto-oncogene encodes a nonreceptor protein tyrosine kinase that is ubiquitously expressed and highly conserved in metazoan evolution. c-Abl protein is distributed in both the nucleus and the cytoplasm of cells. It is implicated in regulating cell proliferation, differentiation, apoptosis, cell adhesion and stress responses (1-3). c-Abl kinase activity is increased in vivo by diverse physiological stimuli including integrin activation, PDGF stimulation and binding to c-Jun, Nck and RFX1 (2,4). The in vivo mechanism of regulation of c-Abl kinase activity is not completely understood. Tyr245 is located in the linker region between the SH2 and catalytic domains. This positioning is conserved among Abl family members. Phosphorylation of Tyr245 is involved in the activation of c-Abl kinase (5). In addition, phosphorylation of Tyr412, which is located in the kinase activation loop of c-Abl, is required for kinase activity (6). Phosphorylation of c-Abl on Tyr89 was identified at Cell Signaling Technology (CST) using PhosphoScan® , CST's LC-MS/MS platform for phosphorylation site discovery as well as another publication using MS technology (7). For additional information please visit PhosphoSitePlus® , CST's modification site knowledgebase, at www.phosphosite.org.
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| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063. For Research Use Only. Not For Use In Diagnostic Procedures. |
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