- 询价
- Cell Signaling Technology
- 4396
- USA
- 2025年12月21日
- W, IP, IF-F
- M,R
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
STEP (23E5) Mouse mAb
- 抗原：
synthetic peptide corresponding to the amino terminus of rat STEP46 protein
- 应用范围：
W, IP, IF-F
- 适应物种：
M,R
- 库存：
大量
- 保质期：
详见说明书
- 供应商：
CST
- 级别：
详见MSDS文件
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IF-F=Immunofluorescence (Frozen)
Reactivity Key: M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IF-F | M R | Endogenous | 46, 61 | Mouse IgG1 |
| Protocols | |
|---|---|
| Specificity / Sensitivity | STEP (23E5) Mouse mAb detects endogenous levels of total STEP46 and STEP61 protein. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of rat STEP46 protein. Western Blotting
Western blot analysis of extracts from adult rat and fetal rat brain using STEP (23E5) Mouse mAb. IF-F
Confocal immunofluorescent analysis of rat hippocampus (left) and striatum (right) using STEP (23E5) Mouse mAb (green) and GFAP (GA5) Mouse mAb (Alexa Fluor® 647 Conjugate) #3657 (blue). Red = propidium iodide (fluorescent DNA dye). |
| Background | Striatal enriched phosphatase (STEP, also known as PTPN5), is a protein tyrosine phosphatase expressed in dopaminoceptive neurons of the central nervous system (1). Alternative splicing produces the cytosolic STEP46 and the membrane-associated STEP61 isoforms of STEP. Dopamine activates D1 receptors and PKA, which in turn phosphorylate both isoforms of STEP. Phosphorylation of STEP61 occurs at Ser160 and Ser221, while STEP46 is phosphorylated at Ser49 (equivalent to Ser221 of STEP61) (2). NMDA-mediated activation of STEP is an important mechanism for regulation of Erk activity in neurons (3). Furthermore, STEP is involved in the regulation of both NMDAR and AMPAR trafficking (4,5). Due to its importance in cognitive function, STEP may play a role in Alzheimer's disease (1). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
T-Cell Activation Using mAb to CD3
One of the most common ways to assess T cell activation is to measure T cell proliferation upon in vitro stimulation of T cells via antigen or agonistic antibodies to TCR. This protocol is written as a starting point for examining in vitro proliferation of mouse
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
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